本文關(guān)鍵詞： 外泌體 血清 肺癌 診斷標(biāo)志物 miR-　出處：《實(shí)用醫(yī)學(xué)雜志》2017年16期 　論文類型：期刊論文

【摘要】：目的探索外泌體miR-21在肺癌患者血清中的表達(dá)水平及診斷效能。方法收集南方醫(yī)院肺癌患者94例,肺結(jié)核患者29例,健康對照26例。純化鑒定血清外泌體,提取miRNA,進(jìn)行RT-PCR,比較各組間miR-21表達(dá)水平。利用ROC曲線評價外泌體miR-21診斷效能。結(jié)果純化樣品具備已有報道的外泌體的典型特征。外泌體miR-21表達(dá)水平在肺癌、肺結(jié)核、健康對照3組間存在顯著性差異;外泌體miR-21在肺癌組中顯著升高,肺結(jié)核組顯著降低。肺癌組中,早、晚期肺癌患者外泌體miR-21與健康對照間均存在顯著性差異。外泌體miR-21的ROC曲線的曲線下面積為0.702,且具有較好的特異性。結(jié)論血清外泌體miR-21具有較高的診斷效能,有望作為診斷標(biāo)志物,輔助肺癌臨床診斷。
[Abstract]:Objective to investigate the expression level and diagnostic efficacy of exocrine miR-21 in serum of lung cancer patients. Methods 94 patients with lung cancer, 29 patients with pulmonary tuberculosis and 26 healthy controls were collected. MiRNAs were extracted, and the expression level of miR-21 was compared among the groups. The diagnostic efficacy of miR-21 was evaluated by ROC curve. Results the purified samples had the typical characteristics of exocrine bodies. The miR-21 expression levels of exosomes were found in lung cancer, pulmonary tuberculosis, pulmonary tuberculosis, lung cancer, pulmonary tuberculosis, pulmonary tuberculosis, lung cancer, pulmonary tuberculosis, pulmonary tuberculosis, pulmonary tuberculosis and lung cancer. The exocrine miR-21 in lung cancer group was significantly higher than that in pulmonary tuberculosis group, while in lung cancer group, it was early in lung cancer group. There was significant difference between exocrine miR-21 and healthy control in patients with advanced lung cancer. The area of ROC curve of exocrine miR-21 was 0.702, and it had good specificity. Conclusion Serum exocrine miR-21 has higher diagnostic efficacy. It is expected to be used as a diagnostic marker to assist the clinical diagnosis of lung cancer.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院臨床醫(yī)學(xué)實(shí)驗(yàn)研究中心;南方醫(yī)科大學(xué)南方醫(yī)院檢驗(yàn)科;
【基金】：廣東省自然科學(xué)基金(編號:2015A030313293) 廣東省科技計劃項(xiàng)目(編號:2013B021800314) 廣州市科技計劃項(xiàng)目(編號:1563000220)
【分類號】：R730.43;R734.2

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