本文關鍵詞： 中頻交變電流 電針 紫杉醇 人乳腺癌MCF-7細胞 存活率 細胞周期 凋亡　出處：《北京中醫(yī)藥大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:研究中頻交變電流聯(lián)合紫杉醇對人乳腺癌MCF-7細胞的作用效果。方法:將對數(shù)生長期的乳腺癌MCF-7細胞分別暴露于電刺激、紫杉醇、電刺激與紫杉醇聯(lián)合作用下,采用cck8法檢測各組細胞存活率,觀察不同中頻交變電流對MCF-7細胞增殖的作用、不同紫杉醇藥物濃度影響MCF-7細胞的生長情況、中頻交變電流聯(lián)合紫杉醇對MCF-7細胞增殖的影響、中頻交變電流聯(lián)合紫杉醇作用MCF-7細胞24h后的細胞恢復時間,并運用流式細胞技術分析各組實驗作用下對細胞凋亡/死亡、細胞周期的影響。結果:1、隨著電流(50—150 mA)的增加,乳腺癌MCF-7細胞的存活率越來越低,且各實驗組的存活率與對照組相比,均有顯著統(tǒng)計學差異(P0.01)。2、與對照組相比,隨著紫杉醇藥物濃度(0.01nmol/L—1000nmol/L)的增加,紫杉醇對MCF-7人乳腺癌細胞的抑制率越來越強,各紫杉醇實驗組均有顯著統(tǒng)計學差異(P0.01),紫杉醇對MCF-7細胞的IC50為31nmol/L。3、中頻交變電流(100 kHz,110 mA,30 min)作用MCF-7細胞后,細胞存活率為70±5.24%;聯(lián)合紫杉醇(IC50)治療后,細胞存活率為24±1.81%(P0.01)。4、各實驗組在24h內治療后,均可見對MCF-7細胞有不同程度的抑制作用。其中,與對照組相比電刺激組及紫杉醇組的人乳腺癌MCF-7細胞減少了近30%,聯(lián)合組的人乳腺癌MCF-7細胞減少了近50%,達到半數(shù)抑制量(P0.01)。在接下來的48h期間,電刺激組相對于藥物組細胞恢復較快;相對于電刺激組,聯(lián)合組和藥物組恢復較慢;最終,聯(lián)合組細胞存活率最低,僅33.73±4.68%(P0.01)。5、電刺激組及紫杉醇組能夠促進MCF-7細胞凋亡,在中頻交變電流聯(lián)合紫杉醇作用后,人乳腺癌MCF-7細胞凋亡率明顯增加,與其它三組相比有顯著性差異(P0.01)。6、與對照組相比,各實驗組G0/G1期細胞明顯減少,S期細胞明顯增多,其中,聯(lián)合治療組G2/M期細胞明顯增多(P0.01)。結論:中頻交變電流單獨使用可以抑制MCF-7細胞增殖,紫杉醇與其聯(lián)合應用時能表現(xiàn)出協(xié)同抗腫瘤細胞作用。
[Abstract]:Objective: to study the effect of intermediate frequency alternating current combined with paclitaxel on human breast cancer MCF-7 cells. Methods: MCF-7 cells in logarithmic phase were exposed to electrical stimulation and paclitaxel respectively. Under the combination of electric stimulation and paclitaxel, the survival rate of MCF-7 cells was detected by cck8 assay, and the effects of different intermediate frequency alternating currents on the proliferation of MCF-7 cells were observed. The growth of MCF-7 cells was affected by different concentrations of paclitaxel, and the proliferation of MCF-7 cells was influenced by intermediate frequency alternating current combined with paclitaxel. The recovery time of MCF-7 cells after 24 hours of treatment with intermediate frequency alternating current combined with paclitaxel was analyzed by flow cytometry. Results: the survival rate of breast cancer MCF-7 cells was lower and lower with the increase of electric current 50-150 Ma, and the survival rate of each experimental group was higher than that of the control group. Compared with the control group, the concentration of paclitaxel increased with 0.01nmol / L ~ (-1) nmol / L). The inhibitory rate of paclitaxel on MCF-7 human breast cancer cells was stronger and stronger, and there was a significant difference among each paclitaxel experimental group (P0.01). The IC50 of paclitaxel on MCF-7 cells was 31nmol / L ~ (3), and the medium frequency alternating current was 100kHz ~ 110mA ~ (-1) for 30 min). The cell survival rate was 70 鹵5.24; The cell survival rate was 24 鹵1.81% after treatment with paclitaxel IC50, and the survival rate of each experimental group was 24 鹵1.81% within 24 hours. Compared with the control group, the number of MCF-7 cells in the electrical stimulation group and paclitaxel group decreased by nearly 30%. In the combined group, the MCF-7 cells of breast cancer decreased by nearly 50%, reaching the half inhibitory amount (P 0.01). During the next 48 hours, the cells recovered faster in the electric stimulation group than in the drug group. Compared with the electrical stimulation group, the recovery was slower in the combined group and the drug group. In the end, the survival rate of the combined group was the lowest, only 33.73 鹵4.68% (P 0.01). The electric stimulation group and paclitaxel group could promote the apoptosis of MCF-7 cells. The apoptosis rate of human breast cancer MCF-7 cells increased significantly after the treatment of intermediate frequency alternating current combined with paclitaxel, which was significantly different from the other three groups (P 0.01) and compared with the control group (P < 0.05). The number of G _ 0 / G _ 1 phase cells in each experimental group was significantly decreased, and the number of S phase cells was significantly increased. In the combined treatment group, the number of G2 / M phase cells increased significantly (P 0.01). Conclusion: the intermediate frequency alternating current alone can inhibit the proliferation of MCF-7 cells. Paclitaxel combined with paclitaxel showed synergistic anti-tumor effect.
【學位授予單位】：北京中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.9

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