本文關(guān)鍵詞： 癌 非小細(xì)胞肺 細(xì)胞角蛋白 免疫組織化學(xué) Logistic回歸分析　出處：《山東大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年03期 　論文類型：期刊論文

【摘要】：目的探討細(xì)胞角蛋白14(CK14)基因的表達(dá)在非小細(xì)胞肺癌(NSCLC)發(fā)生、發(fā)展及轉(zhuǎn)移中的意義。方法采用免疫組織化學(xué)染色法檢測(cè)110例NSCLC患者癌組織、癌旁組織、正常肺組織中CK14的表達(dá)。采用Western blotting法檢測(cè)12例患者新鮮組織中CK14的表達(dá)。應(yīng)用非條件Logistic回歸分析CK14表達(dá)水平與NSCLC患者各臨床病理參數(shù)的關(guān)系。結(jié)果免疫組織化學(xué)染色結(jié)果顯示,CK14在NSCLC癌組織中高表達(dá),陽(yáng)性表達(dá)率為68.18%,與癌旁組織和正常肺組織相比,差異有統(tǒng)計(jì)學(xué)意義(P0.001)。CK14表達(dá)水平與NSCLC患者性別、組織類型、分化程度、分期以及淋巴結(jié)轉(zhuǎn)移情況顯著相關(guān)(P0.05),與年齡無(wú)關(guān)(P0.05)。Western blotting結(jié)果顯示,CK14在NSCLC癌組織中的表達(dá)率較癌旁組織和正常肺組織高。非條件Logistic分析顯示,低分化癌、病理分期Ⅱ期和Ⅲ期、CK14表達(dá)陽(yáng)性是NSCLC患者發(fā)生淋巴結(jié)轉(zhuǎn)移的重要危險(xiǎn)因素(P0.05)。結(jié)論 CK14表達(dá)在NSCLC的發(fā)生、發(fā)展和轉(zhuǎn)移中發(fā)揮重要作用。CK14有望成為評(píng)估NSCLC生物學(xué)行為的重要指標(biāo)。
[Abstract]:Objective to investigate the expression of cytokeratin 14 (CK14) gene in non-small cell lung cancer (NSCLC). Methods Immunohistochemical staining was used to detect the cancerous and paracancerous tissues in 110 patients with NSCLC. Expression of CK14 in normal lung tissue with Western. Blotting method was used to detect the expression of CK14 in fresh tissues of 12 patients. The expression level of CK14 and the clinicopathological parameters of NSCLC were analyzed by non-conditional Logistic regression analysis. Results Immunohistochemical staining showed that. The expression rate of CK14 was 68.18 in NSCLC tissues, which was higher than that in adjacent tissues and normal lung tissues. There was a significant correlation between the expression of P0.001P. CK14 and sex, tissue type, differentiation, stage and lymph node metastasis of NSCLC patients (P 0.05). The results of Western blotting showed that there was no correlation between age and age. The expression rate of CK14 in NSCLC was higher than that in paracancerous tissues and normal lung tissues. Non-conditional Logistic analysis showed that the expression of CK14 in poorly differentiated carcinoma, pathological stage 鈪，

本文編號(hào)：1465166