本文關(guān)鍵詞： 畸胎瘤源性細(xì)胞生長因子 肺癌 腫瘤標(biāo)志物　出處：《蘇州大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討肺癌患者血清畸胎瘤源性細(xì)胞生長因子(PCDGF)、血管內(nèi)皮生長因子(VEGF)及細(xì)胞質(zhì)胸苷激酶1(TK1)與肺癌臨床病理參數(shù)的相關(guān)性,并判斷其在肺癌聯(lián)合診斷中的價值。方法:采用酶聯(lián)免疫吸附試驗(ELISA)法檢測123例肺癌患者、60例肺良性疾病患者和61例健康體檢者血清PCDGF、VEGF的含量,并分析其與臨床病理參數(shù)的相關(guān)性;同時采用酶免疫點(diǎn)印跡化學(xué)發(fā)光法檢測標(biāo)本中TK1的含量。結(jié)果:肺癌組血清PCDGF、VEGF、TK1含量分別為18.27(6.24,58.86)ng/m L、1072.73(272.42,2686.46)ng/m L和1.59(0.21,8.21)pmol/L,均顯著高于肺良性疾病組及健康對照組(Z分別為81.349、85.769和18.818,P均0.05)。血清PCDGF含量與腫瘤分期、淋巴結(jié)轉(zhuǎn)移均有統(tǒng)計學(xué)意義(Z分別為8.063,2.488,P均0.05)。VEGF含量對區(qū)分不同組織學(xué)類型肺癌具有統(tǒng)計學(xué)意義(Z=6.634,P0.05)。經(jīng)直線相關(guān)性分析得出:血清PCDGF與VEGF含量間無相關(guān)性(r=0.017,P=0.786)。PCDGF在肺癌診斷中的敏感性(83.7%)和準(zhǔn)確性(70.9%)均高于TK1的27.3%和49.5%,TK1的特異性(90.7%)高于PCDGF的60.3%。三者聯(lián)合的敏感性最高為95.9%。結(jié)論:PCDGF、VEGF、TK1含量在肺癌患者血清中顯著增高。PCDGF與肺癌分期、淋巴結(jié)轉(zhuǎn)移相關(guān),PCDGF診斷肺癌的敏感性高于VEGF及TK1,提示對肺癌患者血清中PCDGF的檢測具有一定的臨床應(yīng)用價值,可作為肺癌診斷及惡性程度評價的潛在血清標(biāo)志物。
[Abstract]:Objective: to study serum teratoma derived cell growth factor (PCDGF) in patients with lung cancer. The correlation between vascular endothelial growth factor (VEGF) and cytoplasmic thymidine kinase (TK1) and clinicopathologic parameters of lung cancer. Methods: Enzyme-linked immunosorbent assay (Elisa) was used to detect 123 patients with lung cancer. The serum levels of PCDGFV VEGF in 60 patients with benign pulmonary diseases and 61 healthy controls were analyzed and their correlation with clinicopathological parameters was analyzed. At the same time, the content of TK1 in lung cancer samples was detected by enzyme-immune dot blot chemiluminescence assay. Results: the serum levels of TK1 in lung cancer group were 18.27 ~ 6.24, respectively. 58.86 ng / m L ~ (10 72.73) ~ 272.42 ~ 2686.46 ng / m ~ (-1) and 1.59 ~ (0.21) ~ 8.21 g 路mol ~ (-1) 路L ~ (-1). They were significantly higher than those in benign lung disease group and healthy control group (81.349) 85.769 and 18.818 (P < 0.05), respectively. The serum PCDGF content and tumor stage were significantly higher than those in benign lung disease group and healthy control group. There was significant difference in lymph node metastasis between the two groups (8.063n 2.488). P < 0.05. VEGF content was statistically significant in differentiating different histological types of lung cancer. The linear correlation analysis showed that there was no correlation between serum PCDGF and VEGF content. The sensitivity and accuracy of TK1 in the diagnosis of lung cancer were higher than those of TK1 (27.3% and 49.5%). The specificity of TK1 was 90.7 higher than that of PCDGF 60.3.The sensitivity of the three combinations was 95.90.Conclusion the sensitivity of TK1 was 95.9%. The level of TK1 in serum of lung cancer patients was significantly higher than that in patients with lung cancer. The sensitivity of lymph node metastasis related TK1 in diagnosis of lung cancer was higher than that of VEGF and TK1. The results suggest that the detection of PCDGF in serum of lung cancer patients has some clinical application value and can be used as a potential serum marker for the diagnosis of lung cancer and the evaluation of malignancy.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R734.2

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本文編號：1461573