本文關(guān)鍵詞：姜黃素對(duì)Lewis肺癌荷瘤小鼠放療致機(jī)體損傷的保護(hù)作用及機(jī)制研究　出處：《吉林大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 姜黃素 放療 外周血細(xì)胞 骨髓細(xì)胞 NK細(xì)胞 Bax、Bcl-2表達(dá)

【摘要】：目的：本研究觀察姜黃素對(duì)Lewis肺癌荷瘤小鼠的腫瘤放療增敏及放療致機(jī)體損傷的保護(hù)雙向作用及其機(jī)制研究。 方法：C57BL/6純系小鼠，雌雄各半，，制備小鼠Lewis肺癌體內(nèi)移植腫瘤模型，按體重將荷瘤小鼠隨機(jī)分成四組，即空白對(duì)照組、單純放療組、單純姜黃素組和姜黃素+放療聯(lián)合組。末次給藥后稱取小鼠體重，測(cè)量腫瘤重量及體積，測(cè)定各組Lewis肺癌荷瘤小鼠外周血白細(xì)胞數(shù)量、骨髓有核細(xì)胞計(jì)數(shù)、胸腺及脾指數(shù)，同時(shí)測(cè)定荷瘤小鼠T淋巴細(xì)胞轉(zhuǎn)化率、NK細(xì)胞殺傷活性及Bax、Bcl-2蛋白表達(dá)水平。 結(jié)果：①與空白對(duì)照組比較，單純放療組荷瘤小鼠體重、瘤重及腫瘤體積明顯降低（P0.05），聯(lián)合應(yīng)用姜黃素和放射治療可使荷瘤小鼠瘤重、腫瘤體積明顯降低（P0.01）；與單獨(dú)放射治療比較，聯(lián)合應(yīng)用可對(duì)抗放療致荷瘤小鼠體重降低（P0.05），且荷瘤小鼠瘤重、腫瘤體積明顯下降（P0.05），腫瘤抑制率顯著升高（P0.01）；②與空白對(duì)照組比較，在Lewis肺癌腫瘤模型中，單純放療組在發(fā)揮抗腫瘤作用的同時(shí)可見(jiàn)臟器指數(shù)、外周血白細(xì)胞總數(shù)及骨髓有核細(xì)胞數(shù)均明顯下降（P0.05或P0.01）；而姜黃素與放射治療合用可對(duì)抗放療所致的胸腺及脾等免疫器官萎縮、白細(xì)胞及骨髓有核細(xì)胞數(shù)下降，減輕放射治療所產(chǎn)生的毒性（P0.05或P0.01）；③與空白對(duì)照組比較，單純放療組ConA誘導(dǎo)的小鼠淋巴細(xì)胞增殖反應(yīng)及小鼠NK細(xì)胞活性明顯降低（P0.05），姜黃素與放射治療合用，可使ConA誘導(dǎo)的小鼠淋巴細(xì)胞增殖反應(yīng)顯著升高（P0.05），亦可明顯提高小鼠NK細(xì)胞活性（P0.05）；④與空白對(duì)照組比較，單純放療組荷瘤小鼠腫瘤組織中Bax蛋白表達(dá)量明顯升高（P0.01），Bcl-2蛋白表達(dá)量明顯降低（P0.01），Bax/Bcl-2比率升高（P0.05）；聯(lián)合應(yīng)用姜黃素和放射治療可使荷瘤小鼠Bax蛋白表達(dá)量明顯升高（P0.01），Bcl-2蛋白表達(dá)量明顯降低（P0.01），Bax/Bcl-2比率明顯升高（P0.05），且作用優(yōu)于放射治療組（P0.01）。 結(jié)論：姜黃素與放射治療聯(lián)合應(yīng)用具有腫瘤放療增敏作用，還能對(duì)正常組織的輻射損傷有保護(hù)作用，其機(jī)制可能與抗貧血、升高白細(xì)胞、免疫調(diào)節(jié)作用及調(diào)節(jié)Bax、Bcl-2蛋白表達(dá)水平誘導(dǎo)凋亡有關(guān)。
[Abstract]:Objective: To observe the effect and mechanism of curcumin on the protective effect of curcumin on radiation sensitization and injury induced by radiotherapy in Lewis lung cancer mice.
Methods: C57BL/6 mice, male and female, the preparation of Lewis lung cancer in mice transplanted tumor model, according to the weight of the tumor bearing mice were randomly divided into four groups: control group, radiotherapy group, pure curcumin group and the curcumin + radiotherapy group. After the last administration said the mice weight, tumor weight and volume measurement, quantitative determination of tumor bearing mice of Lewis lung cancer were peripheral white blood cells, bone marrow nucleated cell count, thymus and spleen index were measured at the same time, the conversion rate of T lymphocytes of tumor bearing mice, NK cell activity and Bax level, the expression of Bcl-2 protein.
Results: compared with control group, radiotherapy group, tumor bearing mice weight, tumor weight and tumor volume decreased significantly (P0.05), the combined application of curcumin and radiation therapy to tumor bearing mice tumor weight, tumor volume decreased significantly (P0.01); compared with radiotherapy alone, radiotherapy combined against induced tumor the body weight decreased (P0.05), and the tumor bearing mice tumor weight, tumor volume decreased significantly (P0.05), the tumor inhibition rate was significantly increased (P0.01); second, compared with the control group, in Lewis lung cancer model, radiotherapy group in play anti-tumor effect and visible organ index, peripheral blood the total cell number and the number of bone marrow nucleated cells were significantly decreased (P0.05 or P0.01); and curcumin and radiotherapy combined with immune organs against radiation-induced thymus and spleen atrophy, white blood cell and bone marrow nucleated cell number decreased, reduce the radiation therapy produced The toxicity (P0.05 or P0.01); and compared with the control group, the activity of lymphocyte proliferation in mice and mouse NK cells induced by ConA irradiation group decreased significantly (P0.05), combination of curcumin and radiation therapy, lymphocyte proliferation reaction induced by ConA can be significantly increased (P0.05), can significantly increase the activity of NK cells mouse (P0.05); and compared with the control group, the expression of Bax protein was significantly higher in the radiotherapy group in tumor tissues (P0.01), Bcl-2 protein expression was significantly decreased (P0.01), the ratio of Bax/Bcl-2 increased (P0.05); combined application of curcumin and radiation therapy to tumor bearing mice Bax protein expression significantly increased (P0.01), Bcl-2 protein expression was significantly reduced (P0.01), Bax/Bcl-2 ratio was significantly increased (P0.05), and the effect is better than that of radiotherapy group (P0.01).
Conclusion: Curcumin combined with radiotherapy can enhance the sensitivity of radiotherapy and protect the radiation injury of normal tissues. The mechanism may be related to anti anemia, increased white blood cells, immunomodulatory effect, and regulate the expression level of Bax and Bcl-2 protein, and induce apoptosis.

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R730.5

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