本文關(guān)鍵詞：胃癌組織中CD44v6、MPP-9蛋白的表達(dá)及其與腫瘤浸潤(rùn)及轉(zhuǎn)移的關(guān)系　出處：《鄭州大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 胃癌 基質(zhì)金屬蛋白酶-9(MMP-9) CD44v6白細(xì)胞分化抗原分化群44第6亞群 雙重免疫組化染色

【摘要】：[背景]：惡性腫瘤的侵襲和轉(zhuǎn)移是引起患者死亡的主要原因,而腫瘤侵襲和轉(zhuǎn)移是個(gè)多步驟多基因參與的復(fù)雜過程,其中細(xì)胞黏附、基質(zhì)溶解和細(xì)胞移動(dòng)是癌細(xì)胞侵襲轉(zhuǎn)移過程中的3個(gè)關(guān)鍵步驟。近年來(lái)研究表明,CD44v6和MMP-9參與了腫瘤的發(fā)生發(fā)展過程,特別是淋巴結(jié)轉(zhuǎn)移以及浸潤(rùn)性生長(zhǎng),同時(shí)還發(fā)現(xiàn)他們?cè)谀[瘤中的表達(dá)與腫瘤不良預(yù)后可能有著密切的關(guān)系。[目的]：本研究目的是檢測(cè)CD44v6和MMP-9在60例原發(fā)性胃癌組織及60例癌旁組織中的表達(dá)情況,并收集其臨床病例資料,分析其在胃癌組織中的表達(dá)與胃癌浸潤(rùn)轉(zhuǎn)移的關(guān)系以及二者在胃癌浸潤(rùn)轉(zhuǎn)移過程中的相關(guān)性。[方法]：收集鄭州大學(xué)第一附屬醫(yī)院2013年3月至2014年3月期間收治的病理資料及臨床資料完整的胃癌根治手術(shù)病人60例,所有病例手術(shù)前均未曾接受過放、化療、中醫(yī)及免疫治療。選取胃癌組織標(biāo)本60例,以及癌旁組織60例,(距癌組織大于5厘米),制作蠟塊。采用雙重免疫組化染色檢測(cè)CD44v6與MMP-9在胃癌中及相應(yīng)癌旁正常組織中的表達(dá),數(shù)據(jù)分析用卡方檢驗(yàn)比較二者在不同組織中的表達(dá),卡方檢驗(yàn)檢驗(yàn)胃癌組織中CD44v6和MMP-9蛋白質(zhì)的表達(dá)與腫瘤患者臨床病理特征之間的關(guān)系及二者在胃癌組織中表達(dá)的相關(guān)性。所有的統(tǒng)計(jì)分析采用SPSSv17.0。[結(jié)果]：1、在胃癌組織中CD44v6蛋白的陽(yáng)性表達(dá)率是81.67%,顯著高于在癌旁組織中的陽(yáng)性表達(dá)率20.00%,兩者之間差異具有統(tǒng)計(jì)學(xué)意義(χ2=53.393 p0.01)2、MMP-9蛋白在胃癌組織中的陽(yáng)性表達(dá)率為73.33%,明顯高于其在癌旁組織中的表達(dá)率5.00%,兩者之間差異有統(tǒng)計(jì)學(xué)意義(χ2=10.568 p0.01)。3、卡方檢驗(yàn)分析結(jié)果表明,CD44v6蛋白的表達(dá)與腫瘤的浸潤(rùn)深度、TNM分期相關(guān)(χ2=9.013和8.104 p0.05)。而與患者的年齡、性別、淋巴結(jié)轉(zhuǎn)移數(shù)目和腫瘤的大小及腫瘤發(fā)生位置無(wú)明顯相關(guān)性(p0.05)4、MMP-9蛋白的表達(dá)與與患者性別、年齡、淋巴結(jié)轉(zhuǎn)移數(shù)目以及腫瘤的大小以及腫瘤的發(fā)生位置均無(wú)明顯相關(guān)性(p0.05)；而與腫瘤的浸潤(rùn)深度、TNM分期相關(guān)(χ2=9.882和8.304 p0.05)。5、胃癌組織中MMP-9與CD44v6蛋白韻表達(dá)具有一定相關(guān)性(r=0.036,p0.01),且二者在胃癌中的表達(dá)呈正相關(guān)。[結(jié)論]：一、CD44v6和MMP-9蛋白在胃癌組織中的表達(dá)水平均高于其在癌旁正常組織,可能在胃癌的進(jìn)展中起到一定的作用。二、CD44v6和MMP-9蛋白在胃癌組織中的高表達(dá)水平與腫瘤浸潤(rùn)深度及臨床TNM分期相關(guān),可能在腫瘤浸潤(rùn)及轉(zhuǎn)移過程中發(fā)揮一定作用。三、胃癌組織中CD44v6和MMP-9的表達(dá)具有相關(guān)性,呈正相關(guān)。兩者可能在腫瘤浸潤(rùn)及轉(zhuǎn)移過程中發(fā)揮協(xié)同作用。
[Abstract]:[background]: the invasion and metastasis of malignant tumor is the leading cause of death, and the invasion and metastasis of tumor is a complicated process of many steps involved in many genes, including cell adhesion, cell migration and invasion is the dissolution of 3 key steps in the process of metastasis of cancer cells. Recent studies indicate that CD44v6 and MMP-9 in the process of tumor development, especially lymph node metastasis and invasive growth, also may be closely related to them. In the cancer and tumor prognosis: the purpose of this study was to detect the expression of CD44v6 and MMP-9 in 60 cases of primary gastric carcinoma and 60 cases of adjacent in the organization, and collect the clinical data, analysis of the relationship between its expression in gastric cancer tissue and gastric cancer invasion and metastasis as well as the two invasion. In the process of transfer correlation method] in gastric cancer: the collection of Zhengzhou Pathology of the First Affiliated Hospital of University from March 2013 to March 2014 and complete clinical data of 60 cases of gastric cancer patients, all patients were not given radiotherapy, chemotherapy, and immunotherapy of gastric cancer. Chinese medicine selected tissue samples from 60 cases and 60 cases of para carcinoma tissues, (from cancer tissue more than 5 cm), wax block. Expression by double immunohistochemical staining of CD44v6 and MMP-9 in gastric carcinoma and adjacent normal tissues, data analysis using chi square test to compare the two expression in different tissues, the relationship between the chi square test of CD44v6 in gastric cancer tissues and the expression of MMP-9 protein and tumor patients pathological features and two expression in gastric cancer tissues by SPSSv17.0.[]:1. Results all the statistical analysis in gastric carcinoma CD44v6 protein positive expression rate was 81.67%, significantly higher than that in The positive expression in cancer tissue was 20%, with a significant difference (2=53.393 P0.01 2), the positive expression of MMP-9 protein in gastric cancer tissues was 73.33%, significantly higher than the expression in adjacent tissues was 5%, there was significant difference between the two (2=10.568 P0.01).3, chi square test results show that the depth of invasion, the expression of CD44v6 protein and tumor, TNM staging (2=9.013 and 8.104 P0.05). The gender and age of patients, tumor metastasis, lymph node size and number and tumor location had no significant correlation (P0.05) 4, MMP-9 protein expression and age and gender. The number of metastatic lymph nodes, the location and the size of the tumor and the tumor was not significantly correlated (P0.05); but with the depth of tumor invasion, TNM staging (2=9.882 and 8.304 P0.05) of.5, MMP-9 in gastric cancer tissues and CD44v6 eggs White rhyme expression has certain correlation (r=0.036, P0.01), the expression was positively correlated. Conclusion] and two in gastric carcinoma: A, the expression level of CD44v6 and MMP-9 protein in gastric cancer tissues were higher than those in the normal and adjacent cancer tissues, may play a role in the progression of gastric cancer. CD44v6 and two. MMP-9 protein in gastric carcinoma tissues with high expression level and the depth of tumor invasion and clinical TNM stage, may be in the process of tumor invasion and metastasis play a certain role. Three, a correlation between the expression of CD44v6 and MMP-9 in gastric carcinoma was positively correlated. Both in tumor invasion and metastasis process play a synergistic effect.

【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.2

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本文編號(hào)：1368837