本文關(guān)鍵詞：ULK1和ANGPTL3在食管鱗狀細(xì)胞癌組織中的表達(dá)及意義　出處：《揚(yáng)州大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： ULK1 ANGPTL3 免疫組化 食管鱗狀細(xì)胞癌 預(yù)后判斷

【摘要】：目的：檢測(cè)ULKl和ANGPTL3在食管鱗狀細(xì)胞癌組織中的表達(dá)及其相關(guān)性,探討兩者在食管鱗狀細(xì)胞癌(ESCC)發(fā)生、發(fā)展中的作用及其臨床預(yù)后判斷價(jià)值。方法：應(yīng)用組織微陣列技術(shù)結(jié)合免疫組織化學(xué)染色方法(EnVision兩步法)檢測(cè)86例食管鱗狀細(xì)胞癌組織以及78例癌旁組織中ULK1和ANGPTL3蛋白的表達(dá)情況,并分析兩者與臨床病理參數(shù)和生存時(shí)間之間的相關(guān)性。結(jié)果：1、在食管鱗狀細(xì)胞癌組織和癌旁組織中,ULK1蛋白表達(dá)陽性率分別為61.6%和23.1%,差異具有統(tǒng)計(jì)學(xué)意義(x2=24.761,p=0.000)；ANGPTL3蛋白表達(dá)陽性率分別為59.3%和14.1%,差異具有統(tǒng)計(jì)學(xué)意義(x2=35.540,p=0.000)。ULKl蛋白表達(dá)水平與TNM分期(x2=6.320,p=0.012)具有顯著的相關(guān)性。ANGPTL3蛋白表達(dá)水平與性別、年齡、腫瘤大小、組織學(xué)分級(jí)、TNM分期、淋巴結(jié)轉(zhuǎn)移(LNM)之間均沒有明顯的相關(guān)性(P0.05)。2、Spearman秩相關(guān)分析顯示,ULK1蛋白表達(dá)與ANGPTL3蛋白表達(dá)呈正相關(guān)(r=0.222,p=0.040)。3、應(yīng)用Kaplan-Meier生存分析和COX單因素分析表明,TNM分期Ⅲ+Ⅳ(危險(xiǎn)比=1.779,95%置信區(qū)間：1.035-3.058,P=0.037)、淋巴結(jié)轉(zhuǎn)移(危險(xiǎn)比=1.765,95%置信區(qū)間：1.026-3.038,P=0.040)、ULK1胞漿陰性表達(dá)(危險(xiǎn)比=0.508,95%置信區(qū)間：0.296-0.873,P=0.014)、ANGPTL3胞漿陽性表達(dá)(危險(xiǎn)比=1.880,95%置信區(qū)間：1.043-3.388,P=0.036)與食管鱗狀細(xì)胞癌患者較差的預(yù)后顯著相關(guān)。COX多因素分析表明,ULK1蛋白表達(dá)水平(危險(xiǎn)比=0.471,95%置信區(qū)間：0.265-0.836,P=0.010)、ANGPTL3蛋白表達(dá)水平(危險(xiǎn)比=2.156,95%置信區(qū)間：1.177-3.950,P=0.013)是食管鱗狀細(xì)胞癌患者的獨(dú)立預(yù)后因素。結(jié)論：1、ULK1和ANGPTL3在食管鱗狀細(xì)胞癌組織中的陽性表達(dá)率明顯高于癌旁組織,且兩者的表達(dá)呈正相關(guān)。提示ULK1和ANGPTL3可能參與了食管鱗狀細(xì)胞癌的發(fā)生、發(fā)展過程。2、ULK1蛋白表達(dá)水平與TNM分期(x2=6.320,p=0.012)具有顯著的相關(guān)性。提示ULK1的表達(dá)可能與食管鱗狀細(xì)胞癌患者惡性生物學(xué)行為相關(guān)。3、ULK1和ANGPTL3與臨床預(yù)后有一定的相關(guān)性,兩者有可能成為食管鱗狀細(xì)胞癌患者預(yù)后判斷的分子標(biāo)志物。
[Abstract]:Objective: To study the expression and correlation between ULKl and ANGPTL3 in esophageal squamous cell carcinoma, to explore both in esophageal squamous cell carcinoma (ESCC), its clinical prognostic value. Methods: by using tissue microarray and immunohistochemistry (EnVision two steps) the expression of ULK1 and ANGPTL3 protein detection 86 cases of esophageal squamous cell carcinoma and 78 cases of adjacent tissues, and to analyze the correlation between clinical and pathological parameters and survival time. Results: 1, the organization in esophageal squamous cell carcinoma and the expression of ULK1 protein, the positive rates were 61.6% and 23.1%, the difference was statistically significant (x2=24.761. P=0.000); the positive rate of ANGPTL3 expression were 59.3% and 14.1%, the difference was statistically significant (x2=35.540, p=0.000).ULKl expression and TNM staging (x2=6.320, p=0. 012) there was significant correlation between.ANGPTL3 expression and gender, age, tumor size, histological grade, TNM staging, lymph node metastasis (LNM) have no obvious correlation between.2 (P0.05), Spearman rank correlation analysis showed that ANGPTL3 was positively correlated with ULK1 expression (r=0.222,.3, p=0.040) application of Kaplan-Meier univariate survival analysis and COX analysis showed that TNM stage III + IV (hazard ratio =1.779,95% confidence interval: 1.035-3.058, P=0.037), lymph node metastasis (hazard ratio =1.765,95% confidence interval: 1.026-3.038, P=0.040), the expression of cytoplasmic ULK1 negative (hazard ratio =0.508,95% confidence interval: 0.296-0.873, P=0.014, ANGPTL3) positive cytoplasm the expression (hazard ratio =1.880,95% confidence interval: 1.043-3.388, P=0.036) and poor prognosis in patients with esophageal squamous cell carcinoma was significantly related to.COX multivariate analysis showed that the expression level of ULK1 protein (hazard ratio =0 .471,95% confidence interval: 0.265-0.836, P=0.010), the expression level of ANGPTL3 protein (hazard ratio =2.156,95% confidence interval: 1.177-3.950, P=0.013) is an independent prognostic factor in patients with esophageal squamous cell carcinoma. Conclusion: 1. ULK1 and ANGPTL3 positive in esophageal squamous cell carcinoma tissues was significantly higher than that in paracancerous tissues, and their expression related. Suggesting that ULK1 and ANGPTL3 may be involved in the carcinogenesis of esophageal squamous cell carcinoma, the development process of.2, the expression level of ULK1 protein and TNM stage (x2=6.320, p=0.012). There was significant correlation between the expression of ULK1 that may be associated with esophageal squamous cell carcinoma patients with malignant biological behavior of.3, ULK1 and ANGPTL3 have a certain correlation with the clinical prognosis, both may become a molecular marker for predicting prognosis of patients with esophageal squamous cell carcinoma.

【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R735.1

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