發(fā)布時(shí)間：2018-02-21 00:58

  本文關(guān)鍵詞： 躁狂癥 丙戊酸鈉 針刺 實(shí)驗(yàn)研究　出處：《廣州中醫(yī)藥大學(xué)》2013年博士論文　論文類(lèi)型：學(xué)位論文

【摘要】：研究背景 我們以針刺對(duì)躁狂大鼠模型對(duì)行為學(xué)和部分中樞神經(jīng)遞質(zhì)水平的影響為研究重點(diǎn),從宏觀和分子生物學(xué)探索針灸治病起效機(jī)制,為明確針灸治療躁狂癥提供實(shí)驗(yàn)依據(jù)。 研究目的 通過(guò)觀察針刺經(jīng)穴、非穴以及丙戊酸鈉對(duì)跺狂大鼠模型體重、進(jìn)食量和睡眠潛伏期的影響,并通過(guò)檢測(cè)大鼠海馬腦區(qū)GABA. Glu含量及EAAT3蛋白表達(dá)、外周血神經(jīng)遞質(zhì)(多巴胺、5-HT)含量變化,初步探討針刺治療躁狂癥的療效及作用機(jī)制。 研究方法 SD大鼠,SPF級(jí),雌雄各半,體重160-200g,共50只,飼養(yǎng)1周后隨機(jī)分為空白對(duì)照組、模型組、丙戊酸鈉組、穴位針刺組和非穴位針刺組,每組10只。采用改良多平臺(tái)睡眠剝奪法(MMPM)建立躁狂模型�？瞻捉M和模型組不做任何干預(yù),穴位針刺穴位組及非穴組大鼠給予針刺干預(yù),每次留針30分鐘,每日1次,丙戊酸鈉組,予丙戊酸鈉300mg/kg體重,溶于2m1純凈水中灌胃,1次/天。水環(huán)境處理7天結(jié)束后觀察各組大鼠的精神、行為、記錄脫離水環(huán)境到第一次進(jìn)入睡眠的時(shí)間(即睡眠潛伏期)和12小時(shí)進(jìn)食量,并進(jìn)行曠場(chǎng)試驗(yàn)。第8天處死所有大鼠,檢測(cè)大鼠海馬腦區(qū)GABA及G1u及基因表達(dá)、外周血多巴胺類(lèi)神經(jīng)遞質(zhì)含量。所有數(shù)據(jù)使用SPSS13.0進(jìn)行統(tǒng)計(jì)分析。 研究結(jié)果 1.針刺穴位組、丙戊酸鈉組與模型組比較,體重、12h進(jìn)食量及情緒興奮差異均有統(tǒng)計(jì)學(xué)意義(P0.01),提示丙戊酸鈉和穴位針刺能緩解躁狂癥對(duì)體重、12h進(jìn)食量及情緒興奮的影響,非穴位組跟模型組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),提示非穴位對(duì)體重、12h進(jìn)食量及情緒興奮無(wú)改善作用。丙戊酸鈉組和穴位組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),對(duì)于針刺及丙戊酸鈉兩者效果差別不大。丙戊酸鈉組、穴位組與非穴位組跟模型組比較,對(duì)睡眠潛伏期的改善差異有極顯著統(tǒng)計(jì)學(xué)意義,提示三種方法均能明顯改善躁狂癥導(dǎo)致的睡眠潛伏期延長(zhǎng)。丙戊酸鈉組和穴位組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.01),對(duì)于緩解躁狂癥導(dǎo)致睡眠潛伏期延長(zhǎng),穴位針刺效果更佳。 2.在調(diào)節(jié)海馬腦區(qū)GLU/GABA含量作用中,丙戊酸鈉組、穴位組跟模型組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。 3.躁狂大鼠模型外周血DA及5-TH水平比較,丙戊酸鈉組、穴位組跟模型組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。 4.針刺穴位治療后EAAT3蛋白表達(dá)增高,可以看到明顯的蛋白電泳條帶,丙戊酸鈉對(duì)照也比較明顯,而非穴組則不明顯。 研究結(jié)論 1.丙戊酸鈉組、會(huì)刺穴位組能有效增加躁狂大鼠體重及12h進(jìn)食量、縮短睡眠潛伏期,緩解情緒興奮,針刺穴位組的治療效應(yīng)略?xún)?yōu)于丙戊酸鈉組,針刺非穴位無(wú)明顯治療效應(yīng)。 2.丙戊酸鈉組及針刺穴位組能有效降低海馬腦區(qū)Glu、提高GABA含量,針刺非穴位無(wú)明顯治療效應(yīng)。 3.丙戊酸鈉組及針刺穴位組能有效降低躁狂大鼠模型外周血DA水平,增高5-TH水平。 4.針刺穴位制狂效應(yīng)可能與增加海馬腦區(qū)EAAT3蛋白表達(dá)有關(guān)。
[Abstract]:Research background. We focus on the effect of acupuncture on behavior and some central neurotransmitters in manic rats. We explore the mechanism of acupuncture and moxibustion in treating mania from macroscopical and molecular biology, and provide experimental basis for the treatment of mania by acupuncture and moxibustion. Research purpose. The effects of acupuncture at acupoints, non-acupoints and sodium valproate on body weight, food intake and sleep latency in rats were observed. The contents of GABA 路Glu and the expression of EAAT3 protein in hippocampus of rats were measured. The changes of peripheral blood neurotransmitters (dopamine 5-HT) were studied in order to explore the therapeutic effect and mechanism of acupuncture on mania. Research method. SD rats with SPF grade, male and female, weight 160-200g, were randomly divided into blank control group, model group, sodium valproate group, acupoint acupuncture group and non-acupoint acupuncture group. There were 10 rats in each group. The manic model was established by modified multi-platform sleep deprivation (MMPM). The blank group and the model group did not do any intervention. The rats of acupoint acupuncture group and non-point group were treated with acupuncture intervention for 30 minutes each time, once a day. Sodium valproate group was given sodium valproate 300 mg / kg body weight, dissolved in 2m-1 purified water once a day. After 7 days of water treatment, the mental and behavior of rats in each group were observed. The time (i.e. sleep latency) and the amount of food consumed for 12 hours from the water environment to the first sleep were recorded, and the open field test was carried out. On the 8th day, all the rats were killed and the GABA, G1u and gene expression in the hippocampal brain of the rats were detected. The contents of dopamine neurotransmitters in peripheral blood were analyzed by SPSS13.0. Research results. 1.Compared with the model group, there were significant differences in food intake and emotional excitement between acupuncture acupoint group and valproate sodium group, suggesting that sodium valproate and acupuncture at acupoint could alleviate the effects of mania on body weight, food intake and emotional excitement in 12 hours. There was no significant difference between the non point group and the model group (P 0. 05), which suggested that the non acupoint had no effect on the body weight intake and emotional stimulation for 12 hours, while the sodium valproate group and the acupoint group had no effect on the improvement. There was no significant difference in the effect of acupuncture and sodium valproate between the two groups. There was significant difference in the improvement of sleep latency between the point group, the point group and the non-point group as compared with the model group, and there was significant difference in the improvement of sleep latency between the two groups. The results indicated that all the three methods could obviously improve the prolonged sleep latency caused by mania, and the difference between valproate sodium group and acupoint group was statistically significant (P 0.01), which was better for relieving mania and prolonging sleep latency. The effect of acupuncture at acupoint was better than that of sodium valproate group. 2. In regulating the content of GLU/GABA in hippocampus, there was a significant difference between the sodium valproate group and the acupoint group as compared with the model group (P 0.01). 3.The levels of DA and 5-TH in peripheral blood of manic rats were compared with those of model group, and the difference was statistically significant in sodium valproate group, acupoint group and model group. 4.After acupuncture, the expression of EAAT3 protein increased, and the protein electrophoresis bands were observed. The control group of valproate sodium valproate was also obvious, but the non-point group was not. Research conclusion. 1.Sodium valproate group, acupoint acupuncture group can effectively increase the body weight and 12h food intake of manic rats, shorten sleep latency and relieve emotional excitement. The therapeutic effect of acupuncture acupoint group is slightly better than that of sodium valproate group, but acupuncture has no obvious therapeutic effect at non-acupoints. 2. Sodium valproate and acupoint acupuncture group can effectively reduce Glu-induced hippocampal area and increase the content of GABA. Acupuncture at non-acupoint has no obvious therapeutic effect. 3. Sodium valproate and acupoint acupuncture group could effectively decrease DA level and increase 5-TH level in peripheral blood of manic rats. 4. The effect of acupuncture on derangement may be related to the increase of EAAT3 protein expression in hippocampus.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R246.6;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王穎,楊駿;針刺風(fēng)池、風(fēng)府穴對(duì)中風(fēng)后遺癥患者腦血流速度的影響[J];中醫(yī)藥臨床雜志;2005年03期

2 陳靜,陸崢;雙相情感障礙藥物治療進(jìn)展[J];國(guó)外醫(yī)學(xué)(精神病學(xué)分冊(cè));2004年03期

3 宗寶玉;陳鐵光;;利培酮口服液合并丙戊酸鈉緩釋片治療老年躁狂癥療效觀察[J];蚌埠醫(yī)學(xué)院學(xué)報(bào);2012年07期

4 劉燕紅;溫膽湯加減治療癲狂癥20例臨床觀察[J];贛南醫(yī)學(xué)院學(xué)報(bào);1997年04期

5 韓耀輝,石守業(yè);加味溫膽湯治療癲狂證的體會(huì)[J];黑龍江中醫(yī)藥;2002年06期

6 侯合云;;中西醫(yī)結(jié)合治療癲狂的效果分析[J];中外醫(yī)療;2012年07期

7 胡國(guó)勛;;喹硫平聯(lián)合碳酸鋰治療老年躁狂癥31例臨床效果觀察[J];吉林醫(yī)學(xué);2011年12期

8 高哲石;;抗躁狂藥物進(jìn)展[J];上海精神醫(yī)學(xué);1991年S1期

9 張志s，

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