本文選題：高血壓 + 運(yùn)動(dòng)訓(xùn)練　； 參考：《中華高血壓雜志》2015年01期

【摘要】：目的觀察運(yùn)動(dòng)訓(xùn)練對(duì)高血壓大鼠動(dòng)脈壓力反射和中樞氧化應(yīng)激的影響,探討運(yùn)動(dòng)訓(xùn)練改善壓力反射功能的中樞機(jī)制。方法雄性自發(fā)性高血壓大鼠(SHR)20只和正常血壓Wistar大鼠24只隨機(jī)分成對(duì)照組和運(yùn)動(dòng)訓(xùn)練組,運(yùn)動(dòng)組大鼠進(jìn)行8周跑臺(tái)運(yùn)動(dòng)。尾套法測(cè)定大鼠收縮壓和心率,靜脈注射苯腎上腺素(PE)和硝普鈉檢測(cè)動(dòng)脈壓力反射敏感性(BRS)。側(cè)腦室給予超氧化物歧化酶(SOD)擬似劑Tempol和SOD抑制劑DETC,測(cè)定給藥前后BRS變化。下丘腦丙二醛含量、總抗氧化能力(T-AOC)以及抗氧化酶SOD與過(guò)氧化氫酶(CAT)活性用于評(píng)估氧化應(yīng)激。結(jié)果運(yùn)動(dòng)前,SHR收縮壓明顯高于Wistar大鼠(P0.01)。運(yùn)動(dòng)訓(xùn)練顯著降低SHR和Wistar大鼠心率,但僅降低SHR收縮壓。SHR的BRS明顯小于Wistar大鼠[PE:(0.89±0.22)比(1.96±0.23)次/(min·mm Hg);硝普鈉:(0.62±0.10)比(1.32±0.23)次/(min·mm Hg),均P0.01]。運(yùn)動(dòng)訓(xùn)練明顯改善SHR的BRS,與SHR對(duì)照組相比,SHR運(yùn)動(dòng)組BRS明顯提高[PE:(1.31±0.26)比(0.89±0.22)次/(min·mm Hg);硝普鈉:(1.22±0.25)比(0.62±0.10)次/(min·mm Hg);均P0.05]。側(cè)腦室給予DETC抵消運(yùn)動(dòng)訓(xùn)練改善SHR之BRS的作用(P0.05),并降低Wistar大鼠BRS(P0.05),而Tempol則改善SHR的BRS(P0.05),使SHR運(yùn)動(dòng)組大鼠BRS正�；�。運(yùn)動(dòng)訓(xùn)練使SHR下丘腦丙二醛含量降低27%(P0.05),同時(shí)使下丘腦T-AOC、SOD和CAT活性分別增加74%、41%和62%(P0.05);運(yùn)動(dòng)訓(xùn)練使Wistar大鼠下丘腦丙二醛含量降低40%(P0.05),并使下丘腦T-AOC、SOD和CAT活性分別增加72%、30%和51%(P0.05)。結(jié)論中樞氧化應(yīng)激抑制動(dòng)脈壓力反射,運(yùn)動(dòng)訓(xùn)練通過(guò)增強(qiáng)腦內(nèi)抗氧化酶活性和抗氧化能力降低中樞氧化應(yīng)激,從而改善高血壓大鼠動(dòng)脈壓力反射功能。
[Abstract]:Objective to observe the effects of exercise training on arterial baroreflex and central oxidative stress in hypertensive rats and to explore the central mechanism of improving baroreflex function by exercise training. Methods Twenty male spontaneously hypertensive rats (SHR) and 24 normal blood pressure Wistar rats were randomly divided into control group and exercise training group. Systolic blood pressure and heart rate, arterial baroreflex sensitivity (BRS) and phenylephrine (PE) were measured by tail cuff method. The changes of BRS before and after administration of superoxide dismutase (SOD) were measured after administration of Tempol and DETC. The contents of malondialdehyde (MDA) in hypothalamus, total antioxidant capacity (T-AOC) and the activities of antioxidant enzyme SOD and catalase (cat) were used to evaluate oxidative stress. Results the systolic blood pressure of SHR before exercise was significantly higher than that of Wistar rats (P 0.01). Exercise training significantly decreased the heart rate of SHR and Wistar rats, but the BRS of SHR was significantly lower than that of SHR [PE: (0.89 鹵0.22) vs (1.96 鹵0.23) times / (min mm Hg], sodium nitroprusside: (0.62 鹵0.10) vs (1.32 鹵0.23) times / (min mm Hg, respectively (P0.01). Exercise training significantly improved BRS of SHR compared with control group [PE: (1.31 鹵0.26) vs (0.89 鹵0.22) / (min mm Hg]; sodium nitroprusside: (1.22 鹵0.25) vs (0.62 鹵0.10) times / (min mm Hg; P0.05]. DETC counteracting exercise training in lateral ventricle improved BRS of SHR (P0.05) and decreased BRS in Wistar rats (P0.05), while Tempol improved BRS of SHR (P0.05), which normalized BRS in SHR exercise group. Exercise training decreased the content of malondialdehyde (MDA) in hypothalamus by 27% (P0.05), and increased the activities of SOD and cat in hypothalamus by 74 1% and 62% (P0.05), respectively, and decreased the content of MDA by 40% (P0.05) in hypothalamus of Wistar rats, and increased the activities of SOD and cat in hypothalamus by 72 30% and 51% (P 0.05), respectively. Conclusion Central oxidative stress can inhibit arterial baroreflex and exercise training can improve arterial baroreflex function by increasing the activity of antioxidant enzymes and reducing central oxidative stress in hypertensive rats.
【作者單位】： 福鼎市醫(yī)院神經(jīng)內(nèi)科;福建醫(yī)科大學(xué)醫(yī)學(xué)技術(shù)與工程學(xué)院康復(fù)治療學(xué)系;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81372111) 福建醫(yī)科大學(xué)教授基金(JS09001)
【分類號(hào)】：R87

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