本文選題：MAOA + μ-VNTR��； 參考：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：研究目的建立MAOA μ-VNTR態(tài)簡便、快速、準(zhǔn)確的分型方法;探討單胺氧化酶A基因(Monoamine oxidase-A,MAO-A)啟動(dòng)子區(qū)域30bp-VNTR(MAOA μ-VNTR)多態(tài)位點(diǎn)與云南漢族、彝族人群軀體攻擊行為的相關(guān)性,揭示攻擊行為遺傳機(jī)制,為預(yù)防控制攻擊行為提供理論依據(jù)。研究方法應(yīng)用熒光標(biāo)記引物擴(kuò)增結(jié)合毛細(xì)管電泳建立M4OAμ-VNTR多態(tài)位點(diǎn)自動(dòng)分型技術(shù),并與傳統(tǒng)的非標(biāo)記引物擴(kuò)增結(jié)合瓊脂糖凝膠電泳檢測(cè)方法比較;應(yīng)用熒光標(biāo)記毛細(xì)管電泳技術(shù),對(duì)495例男性軀體攻擊行為者(云南漢族315例、彝族180例)和527例男性健康對(duì)照(云南漢族366例、彝族161例)進(jìn)行MAOA μ-VNTR基因分型。運(yùn)用SPSS19.0軟件進(jìn)行數(shù)據(jù)統(tǒng)計(jì)。研究結(jié)果1.AAOA μ-NTR自動(dòng)分型方法建立了基于熒光標(biāo)記引物毛細(xì)管電泳檢測(cè)的自動(dòng)分型方法,簡便快速,準(zhǔn)確高效,分型結(jié)果與傳統(tǒng)的瓊脂糖凝膠檢測(cè)法一致。2.流行病學(xué)分析結(jié)果具有軀體攻擊行為者的平均年齡為29.16±9.41歲,其中18～40歲的患者最多,在云南漢族和彝族群體中初中及以下低文化程度群體(P=O.001,OR1)軀體攻擊行為風(fēng)險(xiǎn)增加;未婚或離婚群體、吸煙群體、飲酒群體發(fā)生軀體攻擊行為的風(fēng)險(xiǎn)高于在婚群體、不吸煙群體、不飲酒群體(OR1),但均無統(tǒng)計(jì)學(xué)意義(P0.05)。3.M40A μ-VNTR多態(tài)性分布在云南漢族、彝族群體中只觀察到3R、4R等位基因。在云南漢族群體,3R頻率為0.548、4R頻率為0.452;在云南彝族群體中,3R頻率為0.478、4R頻率為0.522;云南漢族、彝族MAOA μ-VNTR分布具有顯著性差異。4.MAOA μ-VNTR多態(tài)性與軀體攻擊行為的相關(guān)性在云南漢族群體中MAOA μ-VNTR多態(tài)在攻擊組和非攻擊組中的分布無顯著性差異(P=0.703)。在云南彝族群體中M4OA μ-VNTR多態(tài)在攻擊組和非攻擊組中的分布無顯著性差異(P=0.835)。5.MAOA μ-VNTR多態(tài)性與潛在風(fēng)險(xiǎn)因素交互作用分析結(jié)果在云南漢族人群中將文化程度、婚姻狀況、飲酒和吸煙四個(gè)風(fēng)險(xiǎn)因素分別與MAOAμ-VNTR進(jìn)行交互分析,均未發(fā)現(xiàn)四個(gè)風(fēng)險(xiǎn)因素與MAOA μ-VNTR存在交互作用(P0.05),但是Logistic回歸分析發(fā)現(xiàn)低文化程度和吸煙史是軀體攻擊行為的風(fēng)險(xiǎn)因素。在云南彝族人群中將文化程度、婚姻狀況、飲酒和吸煙四個(gè)風(fēng)險(xiǎn)因素分別與MAOAμ-VNTR進(jìn)行交互分析,均未發(fā)現(xiàn)四個(gè)風(fēng)險(xiǎn)因素與MAOA μ-VNTR存在交互作用(P0.05),但是Logistic回歸分析發(fā)現(xiàn)低文化程度和吸煙史是軀體攻擊行為的風(fēng)險(xiǎn)因素。研究結(jié)論1、本課題建立了MAOA μ-VNTR多態(tài)性自動(dòng)分型方法,具有高效、簡便、快速、準(zhǔn)確的優(yōu)點(diǎn)。2、云南漢族、彝族的MAOA μ-VNTR多態(tài)位點(diǎn)與軀體攻擊行為無顯著關(guān)聯(lián)。3、在云南漢族、彝族群體中低文化程度和吸煙史是軀體攻擊行為的風(fēng)險(xiǎn)因素。
[Abstract]:Objective to establish a simple, rapid and accurate method for the typing of MAOA 渭 -VNTR, and to investigate the relationship between 30bp-VNTR(MAOA 渭 -VNTRR polymorphism in Monoamine oxidase-A (MAO-A) promoter region and somatic aggression behavior in Yunnan Han and Yi people. To reveal the genetic mechanism of attack behavior and to provide theoretical basis for preventing and controlling attack behavior. Methods M4OA 渭 -VNTR polymorphic locus typing technique was established by fluorescence labeled primer amplification combined with capillary electrophoresis, and compared with the traditional non-labeled primer amplification method combined with agarose gel electrophoresis, and the fluorescence labeled capillary electrophoresis technique was used to detect the polymorphic loci of M4OA 渭 -VNTR. The MAOA 渭 -VNTR genotyping was performed in 495 male somatic aggression behaviors (315 Han, 180 Yi) and 527 healthy controls (366 Han and 161 Yi). Use SPSS19.0 software to carry on data statistics. Results 1.AAOA 渭 -NTR automatic typing method based on fluorescence labeled primer capillary electrophoresis was established. The method was simple, rapid, accurate and efficient. The typing results were consistent with the traditional agarose gel detection method. The results of epidemiological analysis showed that the average age of the subjects with physical aggression was 29.16 鹵9.41 years old, the most of them were 1840 years old, and the risk of somatic aggression was increased in the lower education groups of Han and Yi nationality in Yunnan province. The risk of somatic aggression in unmarried or divorced population, smoking group and drinking group was higher than that in married group, non-smoking group and non-drinking group, but there was no statistically significant distribution of P0.05A .3.M40A 渭 -VNTR polymorphism in Yunnan Han nationality. Only 3R 4R allele was observed in Yi population. In Yunnan Han population, the frequency of 3R is 0.548R, the frequency of 4R is 0.452, and the frequency of 3R is 0.522 in Yunnan Yi population, the frequency of Han nationality in Yunnan is 0.522, the frequency of 3R is 0.522, the frequency of 4R is 0.522. There was significant difference in the distribution of MAOA 渭 -VNTR in Yi nationality. 4. There was no significant difference in the distribution of MAOA 渭 -VNTR polymorphism between attack group and non-attack group in Yunnan Han population. 4. There was no significant difference between MAOA 渭 -VNTR polymorphism and somatic aggression behavior in Yunnan Han population. There was no significant difference in the distribution of M4OA 渭 -VNTR polymorphism between the attack group and the non-aggressive group in the Yi ethnic group of Yunnan. The results of interaction analysis between the polymorphism of M4OA 渭 -VNTR and the potential risk factors in Yunnan Han population were as follows: the educational level and marital status of the Han nationality in Yunnan were analyzed. Four risk factors of alcohol consumption and smoking were interacted with MAOA 渭 -VNTR. There was no interaction between four risk factors and MAOA 渭 -VNTR. However, Logistic regression analysis showed that low education level and smoking history were risk factors of somatic aggression behavior. Four risk factors, including education, marital status, alcohol consumption and smoking, were interacted with MAOA 渭 -VNTR in Yunnan Yi population. There was no interaction between four risk factors and MAOA 渭 -VNTR, but Logistic regression analysis showed that low education level and smoking history were risk factors of somatic aggression behavior. Conclusion 1. The MAOA 渭 -VNTR polymorphism automatic typing method was established in this study. It has the advantages of high efficiency, simplicity, rapidity and accuracy. There is no significant correlation between MAOA 渭 -VNTR polymorphism locus and somatic aggression behavior in Yunnan Han and Yi nationality, but there is no significant correlation between the polymorphism of MAOA 渭 -VNTR polymorphism and somatic aggression behavior in Yunnan Han nationality. Low education level and smoking history are risk factors of physical aggression in Yi nationality.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：D919.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 何建鋒;洪武;邵陽;韓慧琴;謝斌;;MOAS在住院精神障礙患者暴力風(fēng)險(xiǎn)評(píng)估中的應(yīng)用[J];法醫(yī)學(xué)雜志;2017年01期

2 張璐;劉秀霞;李一丹;李巖;鐘新獻(xiàn);李應(yīng)生;李海英;常國斌;陳國宏;廖和榮;;吐魯番斗雞MAOA基因12個(gè)外顯子的SNP分析[J];畜牧與獸醫(yī);2016年06期

3 李輝;李磊;徐紅梅;趙子琴;劉文斌;周懷谷;;人類暴力攻擊行為的遺傳相關(guān)性研究進(jìn)展[J];法醫(yī)學(xué)雜志;2015年05期

4 許雪冬;;酒精導(dǎo)致精神障礙及精神分裂癥所致腦萎縮的頭顱CT檢查結(jié)果及其臨床相關(guān)因素對(duì)照研究[J];山西醫(yī)藥雜志;2012年10期

5 章雪利;胡峻梅;;修訂版外顯攻擊行為量表用于精神疾病患者危險(xiǎn)行為評(píng)估[J];法醫(yī)學(xué)雜志;2011年05期

6 陳道ng;姚玉華;俞愛青;周峰;劉慶元;李瑞昌;;家庭環(huán)境與社區(qū)青少年暴力攻擊行為的關(guān)系及影響因素分析[J];現(xiàn)代預(yù)防醫(yī)學(xué);2011年14期

7 曹玉萍;李龍飛;趙幸福;張亞林;;兒童攻擊行為與COMT Val158Met及5-HTTLPR基因多態(tài)性關(guān)聯(lián)分析[J];中國當(dāng)代兒科雜志;2011年05期

8 姚玉華;陳道ng;周峰;劉慶元;;父母教養(yǎng)方式與社區(qū)青少年暴力攻擊行為的關(guān)系[J];中國學(xué)校衛(wèi)生;2010年09期

9 余微微;李樹民;梅宏偉;張建平;潘恒足;柯曉燕;茆正洪;譚釗安;;反社會(huì)人格障礙單胺氧化酶A基因VNTR多態(tài)性與家庭環(huán)境的相關(guān)研究[J];中國神經(jīng)精神疾病雜志;2010年01期

10 劉華鋒;劉婭;劉麗麗;陳兆杰;李為群;王繼萍;李波;;單胺氧化酶A基因與暴力攻擊行為關(guān)系[J];中國公共衛(wèi)生;2009年12期

相關(guān)博士學(xué)位論文 前2條

1 張潔旭;MAOA內(nèi)部多位點(diǎn)作用模式及DNA甲基化狀態(tài)與精神障礙的相關(guān)性研究[D];北京協(xié)和醫(yī)學(xué)院;2010年

2 劉華鋒;5-HT和MAOA基因多態(tài)性與攻擊行為的關(guān)聯(lián)性及營養(yǎng)素干預(yù)的實(shí)驗(yàn)研究[D];吉林大學(xué);2010年

相關(guān)碩士學(xué)位論文 前3條

1 杜蓮;精神分裂癥與吸煙的關(guān)系和相關(guān)分子遺傳學(xué)研究[D];重慶醫(yī)科大學(xué);2007年

2 楊林;暴力危險(xiǎn)因素評(píng)估及個(gè)體認(rèn)知行為干預(yù)[D];重慶醫(yī)科大學(xué);2007年

3 姚莉華;罪犯與精神分裂癥患者暴力行為相關(guān)因素研究[D];重慶醫(yī)科大學(xué);2004年

，

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