本文選題：腫節(jié)風 + 小型豬　； 參考：《廣西醫(yī)科大學》2013年碩士論文

【摘要】：目的：觀察分割照射條件下,腫節(jié)風對腮腺照射后活性氧簇(ROS)的清除作用及對腮腺細胞凋亡的影響,探討分割照射下腫節(jié)風對腮腺的防護作用。 方法：(1)實驗對象為實驗用巴馬小型豬,建立起腫節(jié)風干預下的腮腺放射性損傷實驗動物模型。60頭小型豬隨機分成空白對照組(對照組)、單純照射組(單照組)和腫節(jié)風加照射組(藥照組)3組,每組平行分成a、b、c、d四個亞組,分別于照射結束后1d、10d、40d和90d取腮腺組織,藥照組于照射前一周開始給予腫節(jié)風顆粒,直至腮腺組織取出,對照組與單照組給予等量生理鹽水。在全麻狀態(tài)下藥照組跟單照組給予總量為30Gy/(5f·5w)60C0γ射線雙側腮腺照射,對照組不予照射。(2)用豬活性氧(ROS)酶聯免疫分析試劑盒檢測其照射后1d、10d、40d、90dROS的含量及RT-PCR法檢測照射后1d、10d、40d、90d腮腺細胞中Bcl-2、Bax、P53和Caspase-3mRNA表達量的變化：結果：(1)照射后1d,對照組、單照組、藥照組ROS含量分別為62.58,136.75,62.83U/ml,對照組與藥照組差異無統(tǒng)計學意義(P0.01),單照組與藥照組、對照組之間差異有統(tǒng)計學意義(P0.01)；照射后10d,對照組、單照組、藥照組ROS含量分別為64.50,338.5,281.67U/m1,照射后40d,對照組、單照組、藥照組ROS含量分別為62.92,392.83,324.42U/m1,照射后90d,對照組、單照組、藥照組ROS含量分別為62.75,438.92,323.75U/ml,每個時間段三組之間差異均有統(tǒng)計學意義(P0.01),藥照組的ROS含量均低于單照組；(2)在同一時點(同一平行組),bc1-2mRNA表達量為空白組(0.84±0.05,0.89±0.09,0.85±0.06,0.92±0.04)藥照組(0.52±0.09,0.58±0.06,0.63±0.02,0.69±0.07)單照組(0.47±0.07,0.23±0.05,0.31±0.06,0.39±0.09):baxmRNA表達量為單照組(0.92±0.09,1.57±0.05,1.41±0.08,1.33±0.08)藥照組(0.78±0.08,0.69±0.08,0.62±0.07,0.59±0.04)空白組(0.56±0.04,0.48±0.08,0.55±0.09,0.49±0.06)；p53mRNA表達量為單照組(1.55±0.21,2.34±0.36,3.8±0.39,4.0±0.48)藥照組(1.05±0.19,1.86±0.31,2.67±0.36,3.14±0.27)空白組(0.67±0.15,O.73±0.12,0.62±0.37,0.55±0.29)；Caspase-3mRNA表達量為單照組(0.54±0.14,0.62±0.09,0.96±0.17,1.17±0.21)藥照組(0.45±0.06,0.57±0.09,0.84±0.12,0.99±0.15)空白組(0.40±0.06,0.37±0.09,0.29±0.04,0.30±0.05)；bcl-2mRNA表達量在照射結束后10d最低、90d最高；bax蛋白在10d最高、90d最低；藥照組中,bcl-2mRNA表達量逐漸遞增；baxmRNA表達量逐漸遞減(P0.05)。單照組中p53和Caspase-3mRNA的表達量均呈逐漸增高趨勢,藥照組中,p53及Caspase-3mRNA的表達量也呈現逐步升高趨勢,但各時間段表達量均低于單照組(P0.05) 結論：(1)分割照射下,成功建立起腫節(jié)風干預下的腮腺放射性損傷實驗動物模型。(2)腫節(jié)風通過清除小型豬腮腺放射后的ROS活性,能有效減緩腮腺放射損傷。(3)腫節(jié)風抑制腮腺細胞放療后細胞凋亡的作用,可能是通過抑制Bax、P53和Caspase3mRNA的表達,同時上調Bcl-2mRNA的表達來實現。
[Abstract]:Aim: to observe the scavenging effect of Jiefeng on rosy after parotid gland irradiation and its effect on apoptosis of parotid gland cells, and to explore the protective effect of Tetrandra on parotid gland under fractionated irradiation. Methods Bama miniature pig was used as experimental object. 60 miniature pigs were randomly divided into three groups: control group (control group), simple irradiation group (single irradiation group), and swelling section plus irradiation group (drug irradiation group), and 60 miniature pigs were randomly divided into three groups: control group (control group), single irradiation group (single irradiation group) and combined irradiation group (drug irradiation group). Each group was divided into four subgroups in parallel. Parotid tissues were taken at 10 days after irradiation for 40 days and 90 days after irradiation. The control group was given the same amount of normal saline one week before irradiation until the parotid gland tissue was taken out, and the control group and the single irradiation group were given the same amount of normal saline. Under general anesthesia, the total amount of 30Gy/(5f 5w)60C0 緯 -ray irradiation on both sides of parotid gland was given to the drug irradiation group and the single irradiation group. In the control group, the Ros content was detected by enzyme linked immunosorbent assay (Elisa) kit of porcine reactive oxygen species (Ros) and the changes of Bcl-2OBX BaxP53 and Caspase-3mRNA expression in parotid gland cells were detected by RT-PCR assay at 1 day, 10 days after irradiation, 40 days after irradiation and 90 days after irradiation: results one day after irradiation, control group, single irradiation group, and the control group, single irradiation group, the expression of Bcl-2P BaxP53 and BaxP53 in parotid gland cells were determined. The content of ROS was 62.58136.75U / ml in the control group and 62.83U / ml in the control group, respectively. There was no significant difference between the control group and the drug exposure group (P 0.01), but the difference between the single irradiation group and the medicine irradiation group was statistically significant (P 0.01), 10 days after irradiation, the control group, the single irradiation group, the control group and the control group. The ROS content of the drug irradiation group was 64.50338.5n 281.67 U / m 1. The ROS content of the control group, the single irradiation group and the drug irradiation group was 62.92392.83 ~ 324.42 U / m ~ (-1) at 40 days after irradiation, respectively. 90 days after irradiation, the control group, the single irradiation group, the control group, the single irradiation group, The ROS content of the drug exposure group was 62.75438.92 ~ 323.75 U / ml, the difference between the three groups was statistically significant in each time period, and the ROS content in the drug irradiation group was lower than that in the single irradiation group (the same parallel group was 0.84 鹵0.050.89 鹵0.069 鹵0.92 鹵0.92 鹵0.92) at the same time point (0.52 鹵0.09 鹵0.58 鹵0.06 鹵0.63 鹵0.020.69 鹵0.07) at the same time point (0.84 鹵0.050.89 鹵0.069 鹵0.92 鹵0.92 鹵0.92 鹵0.92 鹵0.06 鹵0.06 鹵0.06 鹵0.06 鹵0.69 鹵0.07 in the same parallel group, respectively) in the same time point (0. 84 鹵0. 05 鹵0. 05 鹵0. 05 鹵0. 92 鹵0. 92 鹵0. 92 鹵0. 92). (0.47鹵0.07,0.23鹵0.05,0.31鹵0.06,0.39鹵0.09):baxmRNA琛ㄨ揪閲忎負鍗曠収緇，

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