本文選題：腦損傷　切入點(diǎn)：爆震傷　出處：《解放軍醫(yī)學(xué)雜志》2017年09期 　論文類型：期刊論文

【摘要】：目的建立一種模擬艙室內(nèi)爆炸沖擊波致傷的大鼠顱腦爆震傷模型。方法成年雄性SD大鼠50只,隨機(jī)分為胸腹有保護(hù)3、5、8g TNT當(dāng)量組,無保護(hù)5g TNT當(dāng)量組和正常對(duì)照組(n=10)。將不同當(dāng)量的裸藥懸吊于艙室中心。除正常對(duì)照組以外,其他組大鼠麻醉后,放或不放保護(hù)胸腹裝置,俯臥放置于裸藥下方約31cm處,面向爆源。爆炸后,觀察大鼠存活情況,于爆炸后3h、1d、3d進(jìn)行血清學(xué)和病理學(xué)檢查。結(jié)果從爆后組織損傷和大鼠死亡情況來看,與正常對(duì)照組相比,胸腹有保護(hù)3g TNT當(dāng)量組未見明顯組織損傷,全部存活;5g TNT當(dāng)量組肺組織損傷較輕,腦組織水腫明顯,腦表面血管增粗,有少量斑片狀出血,死亡率為30%;8g TNT當(dāng)量組臟器損傷嚴(yán)重,死亡率80%;胸腹無保護(hù)5g TNT當(dāng)量組肺部損傷嚴(yán)重,幾乎全部當(dāng)場(chǎng)死亡。胸腹有保護(hù)5g TNT當(dāng)量組致傷情況較為符合實(shí)驗(yàn)需求。進(jìn)一步行血清學(xué)和病理檢測(cè)顯示:胸腹有保護(hù)5g TNT當(dāng)量組大鼠腦含水量增加,血清神經(jīng)元特異烯醇化酶(NSE)和S-100β蛋白含量顯著升高,腦皮質(zhì)和海馬區(qū)神經(jīng)細(xì)胞呈壞死或凋亡改變。結(jié)論大鼠在胸腹有保護(hù)的情況下,5g TNT艙室中心爆炸可建立較穩(wěn)定的顱腦爆震傷動(dòng)物模型。
[Abstract]:Objective to establish a model of craniocerebral explosive injury induced by explosion shock wave in cabin. Methods 50 adult male Sprague-Dawley rats were randomly divided into 3 5 g TNT equivalent group with chest and abdomen protection. Unprotected 5 g TNT equivalent group and normal control group were suspended from the center of the compartment. After anesthesia, the rats in other groups were placed on or off the protective chest and abdomen device, lying prone to the naked medicine, about 31cm under the naked medicine, except for the normal control group, the rats in the other groups were anesthetized. After explosion, the survival of rats was observed and serological and pathological examinations were performed 3 days after explosion. Results compared with the normal control group, the tissue injury and death of the rats after explosion were observed. No significant tissue damage was found in the group with 3 g TNT equivalent in chest and abdomen, but the lung tissue was slightly damaged, brain tissue edema was obvious, the blood vessels on the surface of the brain were thickened, and there was a small amount of plaque hemorrhage in the group of survival 5 g TNT equivalent. The mortality rate was 30 g / 8 g TNT equivalent, and the injury of viscera was serious in the group of 30 g / g TNT equivalent. Death rate was 80%. Lung injury was severe in unprotected 5g TNT equivalent group. Almost all of them died on the spot. The injury in the group with 5 g TNT equivalent in chest and abdomen met the need of the experiment. Further serological and pathological examination showed that the water content of brain increased in the group of 5 g TNT equivalent with the protection of chest and abdomen. The contents of serum neuron specific enolase (NSE) and S-100 尾 protein increased significantly. Conclusion under the condition of chest and abdomen protection, the central explosion of 5g TNT compartment can establish a stable animal model of craniocerebral explosive injury.
【作者單位】： 海軍總醫(yī)院神經(jīng)外科;
【基金】：國(guó)家自然科學(xué)基金(81372128) 全軍后勤科研重大項(xiàng)目(AHJ14J001)~~
【分類號(hào)】：R-332;R826

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