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  本文關鍵詞： 電子線照射 SD大鼠 大劑量放療 術(shù)中放療　出處：《石河子大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:使用單次大劑量電子線照射SD大鼠胃,模擬胃部術(shù)中放療(Intraoperative radiotherapy,IORT),觀察大鼠胃照射后的損傷變化及修復情況,為大劑量術(shù)中放療提供動物實驗理論性依據(jù)。方法:38只八周齡健康雄性SD大鼠隨機分為兩組:正常對照組(A組)8只,實驗組(B組)30只。使用專利發(fā)明“小動物放射線精確照射試驗臺[1]”,配合電子線托架,自制1cm厚度,3×3cm2照射野鉛塊,6MeV電子線單次20Gy準確照射B組大鼠胃。動態(tài)觀察大鼠照射后的一般情況、飲食量、飲水量、體重的變化和胃組織粘膜病理學改變。所得體重和飲食量數(shù)據(jù)以均數(shù)±標準差,分別用SPSS 17.0軟件處理,分析結(jié)果。結(jié)果:B組大鼠經(jīng)20Gy電子線單次照射后,受照區(qū)體毛脫落稀疏,前兩周活動減少,精神萎靡,死亡率10%;飲食量在照射后八天內(nèi)較A組減少(P0.05);體重在第一周內(nèi)明顯下降,第二周體重開始增加,體重增加最快在第四周至第六周。第八周時大鼠飲食量已和對照組沒有差異(P0.05),兩組體重比較在第六周時沒有差異(P0.05)。A組飲食量和體重一直相對平穩(wěn)增長。病理大體觀B組大鼠胃,未見穿孔、壞死,胃與周圍組織均有纖維性粘連,在照射后第14天時粘連最重且顏色鮮紅,第56天時最輕且色灰黃。HE染色檢查:B組照射后第14天見,胃粘膜糜爛、粘膜下高度水腫。照射后28天見,粘膜下水腫,纖維化、腺體萎縮。第56天見,胃腺體萎縮,炎性纖維素性滲出。A組胃組織結(jié)構(gòu)完整,粘膜顏色鮮紅、細胞排列整齊、無糜爛,腺體無萎縮,無纖維化及炎細胞浸潤,粘膜下層、肌層和漿膜層均正常。結(jié)論:SD大鼠胃可耐受6MeV電子線單次20Gy照射,無穿孔、壞死等嚴重并發(fā)癥發(fā)生。在照射后,兩周內(nèi)急性反應最重,對體重及飲食影響大,此時,可能是放射性粘膜損傷保護性治療的較佳時機。第八周時已完全形成纖維性修復,提示在照射能量和劑量還可進一步加大,探索大鼠胃受照射能承受的更高劑量。
[Abstract]:Objective: to observe the changes and repair of intragastric radiation induced by intragastric irradiation in SD rats. Methods Thirty eight healthy male Sprague-Dawley rats were randomly divided into two groups: normal control group (n = 8), control group A (n = 8) and control group A (n = 8). Experimental group B (n = 30). Using patent invention "small animal radiation precise irradiation test stand [1]", with electronic wire carrier, The stomach of rats in group B was accurately irradiated with 1 cm thick and 3 脳 3 cm ~ 2 electron rays of 6 MeV electron rays at a single dose of 20 Gy. The general situation, diet and drinking quantity of rats after irradiation were observed dynamically. Weight changes and pathological changes of gastric mucosa. The weight and diet data were treated with SPSS 17.0 software in the mean 鹵standard deviation. Results the body hair in the irradiated area of rats in group B was sparse after 20 Gy electron ray irradiation. During the first two weeks, activity decreased, mental retardation, mortality rate 10. 5% lower than group A in eight days after irradiation, weight decreased significantly in the first week, and weight began to increase in the second week. The maximum weight gain was from the fourth week to 6th weeks. At 8th weeks, there was no difference between the rats and the control group (P 0.05), but there was no difference between the two groups at 6th weeks. The diet and body weight of group A were relatively stable. There was no perforation, necrosis, fibrous adhesion between stomach and surrounding tissue. On the 14th day after irradiation, the adhesion was the heaviest and the color was bright red. On the 56th day, the grayish yellow color was the lightest and grayish yellow. He staining showed that the gastric mucosa was erosive on the 14th day after irradiation in group B. Submucosal high edema. 28 days after irradiation, submucosal edema, fibrosis, atrophy of glands. 56 days, gastric gland atrophy, inflammatory cellulose exudation. Group A gastric tissue structure integrity, mucosal color bright red, cells arranged neatly, no erosion, No atrophy, no fibrosis, no inflammatory cell infiltration, normal submucosal, muscular and serous layers were found in the stomach of the rats. Conclusion the stomach of the SD rats can tolerate a single dose of 20 Gy irradiation with 6 MeV electron lines, no perforation, necrosis and other serious complications. The acute reaction was the most serious in two weeks, which had a great influence on body weight and diet. At this time, it may be a better time for protective treatment of radiation mucosal injury. At 8th weeks, the fibrous repair has been completely formed, suggesting that the radiation energy and dose can be further increased. To explore the higher dose of rat stomach irradiation.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R730.55;R735.2

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