食品中大腸菌群快速檢測效果評價
[Abstract]:Objective to analyze the effect of compound color culture medium on rapid determination of coliform bacteria in food inspection. Methods 85 food samples were studied by using standard strains of coliform group and other standard strains of foodborne pathogenic bacteria. At the same time, the detection method of coliform group and compound color culture method in national standard GB4789.3 were used to detect coliform group, and the detection time of coliform group was compared. Accuracy, etc. The effect of the compound color culture medium was evaluated comprehensively. Results the standard strains of Escherichia coli, citric acid bacillus, Enterobacter cloacae, Enterobacter sakazakii, Enterobacter aerogenes and Klebsiella pneumoniae were inoculated on the compound color plate and VRBA plate, and all of them were blue-green. However, Shigella flexneri, Vibrio parahaemolyticus and Staphylococcus aureus non-coliform bacteria were cultured on the compound color plate for 18 hours, and the colony did not grow. There was no significant difference between the positive rate of food samples and the national standard coliform group VRBA plate method (P 0.05). Conclusion the compound color culture medium can quickly detect coliform bacteria in food and has high accuracy, so it is worth using in food detection.
【作者單位】: 河北經貿大學生物科學與工程學院;
【分類號】:R155.5
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