【摘要】：目的了解南昌地區(qū)兒童手足口病(HFMD)的病原學(xué)構(gòu)成,為該地區(qū)HFMD的流行病學(xué)防控提供基礎(chǔ)。方法收集2014年4至12月臨床送檢疑似HFMD患者咽拭子標(biāo)本,運(yùn)用熒光定量RT-PCR檢測(cè)腸道病毒通用型(EV)、腸道病毒71型(EV71)和柯薩奇病毒A16型(CVA16)核酸;每季度按照等距抽樣的方法選取一定量的非EV71、非CVA16型的其他EV標(biāo)本,利用VP4區(qū)分型引物進(jìn)行巢式PCR,產(chǎn)物通過測(cè)序和Gen Bank Blast比對(duì)進(jìn)行分型鑒定。結(jié)果 2014年4至12月共檢測(cè)到EV陽性病例19 059例,其中EV71、CVA16和其他EV檢出率分別為23.22%(4 425/19 059)、11.36%(2 166/19 059)、65.42%(12 468/19 059)。其他EV抽樣檢測(cè)結(jié)果顯示,第2季度CVA10占比最高,為24.39%(10/41),其次是CVA4和CVA6,各占19.51%(8/41);第3季度CVA6占比最高,為73.08%(95/130),其次是CVA10和CVA4,分別占11.54%(15/130)和6.15%(8/130);而第4季度CVA6占比94.74%(36/38),其次是CVA10占5.26%(2/38)。結(jié)論 2014年第2至第4季度非EV71、非CVA16型的其他EV檢出率最高,且CVA6為2014年第3和第4季度南昌地區(qū)引起HFMD的優(yōu)勢(shì)病毒株。
[Abstract]:Objective to investigate the etiological composition of (HFMD) in children with hand, foot and mouth disease (HFMD) in Nanchang area, and to provide a basis for epidemiological prevention and control of HFMD in Nanchang area. Methods from April to December 2014, throat swabs from suspected HFMD patients were collected and the nucleic acids of enterovirus universal (EV), enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) were detected by fluorescence quantitative RT-PCR (FQ-PCR). According to the method of isometric sampling, a certain number of other EV samples of non-EV71, non-CVA16 type were selected every quarter, and the nesting PCR, products were identified by sequencing and Gen Bank Blast comparison with VP4 discriminant primers. Results from April to December 2014, a total of 19 059 EV positive cases were detected. The positive rates of EV71,CVA16 and other EV were 23.22% (4.425 鈮，

本文編號(hào)：2508925