【摘要】：目的 了解六價鉻[Cr(Ⅵ)]污染地區(qū)地下水對小鼠肝臟、血糖的影響和維生素C（VitC）的干預作用。 方法 將80只健康6～8周齡清潔級雄性昆明小鼠隨機分為8組，分別為對照（蒸餾水）組、鉻污染的井水（采自某被污染的地下水，Cr(Ⅵ)含量為12.35mg/L）組和34.91（Cr(Ⅵ)含量為12.35mg/L）、349.1、698.2、1396.4mg/L重鉻酸鉀單獨染毒組以及Vit C（100mg/ml）+698.2、1396.4mg/L重鉻酸鉀聯合作用組，每組10只。采用經口灌胃方式進行染毒，重鉻酸鉀和井水組的染毒容量為25ml/kg；Vit C的染毒容量為5ml/kg，0.5h后再進行重鉻酸鉀染毒。連續(xù)染毒30d。在試驗過程中觀察小鼠的中毒表現，染毒結束后收集血液和肝組織。血清天門冬氨酸氨基轉移酶（AST）、血糖（Glu）含量、谷丙氨酸氨基轉移酶（ALT）活力用全自動生化分析儀測定；肝臟組織超氧化物歧化酶（SOD）活力和谷胱甘肽（GSH）、丙二醛（MDA）含量用可見分光光度法檢測；HE染色法觀察肝細胞病理形態(tài)學改變。 結果 井水組和各劑量重鉻酸鉀染毒組小鼠在染毒過程中出現了較明顯的中毒癥狀。與對照組比較，井水組和不同濃度重鉻酸鉀單獨染毒組小鼠血清AST、ALT活力和肝臟MDA含量均較高，肝臟SOD活力、GSH含量和血清Glu含量較低，差異有統計學意義（P＜0.05）；而經Vit C預處理各組小鼠血清AST、ALT活力和Glu含量及肝臟SOD活力和GSH、MDA含量無顯著變化；且隨著重鉻酸鉀染毒濃度的升高，小鼠血清AST、ALT活力及肝臟MDA含量呈上升趨勢，，肝臟SOD活力和GSH含量呈下降趨勢。與相同濃度重鉻酸鉀單獨染毒組比較，經VitC預處理各組小鼠血清AST、ALT活力和肝臟MDA含量均較低，血清Glu含量及肝臟SOD活力和GSH含量均較高，差異有統計學意義（P＜0.05）。與34.91mg/L重鉻酸鉀單獨染毒組比較，井水組小鼠血清AST、ALT活力和肝臟MDA含量均較高，血清Glu含量及肝臟SOD活力和GSH含量均較低，差異有統計學意義（P＜0.05）。 結論 Cr(Ⅵ)污染地區(qū)地下水可引起小鼠肝臟的損傷，且可能存在其他重金屬污染；而Vit C對于重鉻酸鉀所致肝損傷有一定的拮抗作用。
[Abstract]:Objective to investigate the effect of groundwater on liver and blood glucose in mice polluted by hexavalent chromium [Cr (VI)] and the intervention effect of vitamin C (VitC). Methods 80 healthy male Kunming mice of 6 weeks old and 8 weeks old were randomly divided into 8 groups: control group (distilled water) group, chromium contaminated well water (12.35mg/L) group (12.35mg/L) and 34.91 (Cr (VI (12.35mg/L) group, respectively, the control group (distilled water), the chromium contaminated well water (12.35mg/L) group and 34.91 (Cr (VI group), the control group (distilled water) and the chromium contaminated well water group (34.91 (Cr (VI) were randomly divided into 8 groups. 349.1698.2, 1396.4 mg 路L potassium dichromate alone and Vit C (100mg/ml) 698.2, 1396.4 mg / L potassium dichromate combined treatment group, 10 rats in each group. The exposure capacity of potassium dichromate and well water group was 25ml kg Vit C was 5ml kg, and then potassium dichromate was exposed to potassium dichromate 0.5 h later. Continuous exposure for 30 days The poisoning performance of mice was observed during the experiment, and the blood and liver tissue were collected after exposure. The contents of blood glucose (Glu) and glutamic alanine aminotransferase (ALT) in serum aspartate aminotransferase (AST),) and glutamic alanine aminotransferase (ALT) were measured by automatic biochemical analyzer, (SOD) activity and malondialdehyde (MDA) content in liver tissue were detected by visible spectrophotometry, and pathomorphological changes of hepatocytes were observed by HE staining. Results the mice in well water group and potassium dichromate group showed obvious poisoning symptoms in the process of exposure. Compared with the control group, the serum AST,ALT activity and liver MDA content of mice exposed to well water group and different concentrations of potassium dichromate alone were higher, while the liver SOD activity, GSH content and serum Glu content were lower, the difference was statistically significant (P < 0.05), but there were no significant changes in serum AST,ALT activity and Glu content, SOD activity and GSH,MDA content in liver of mice pretreated with Vit C. With the increase of potassium dichromate concentration, the activity of serum AST,ALT and the content of MDA in liver of mice increased, while the activity of SOD and the content of GSH in liver decreased. Compared with the group exposed to the same concentration of potassium dichromate alone, the activity of serum AST,ALT and the content of MDA in liver of mice pretreated with VitC were lower, while the contents of serum Glu, SOD activity and GSH in liver were significantly higher than those in group treated with potassium dichromate alone (P < 0.05). Compared with the 34.91mg/L potassium dichromate alone group, the serum AST,ALT activity and liver MDA content in the well water group were higher, while the serum Glu content, liver SOD activity and GSH content were lower, the difference was statistically significant (P < 0.05). Conclusion groundwater in Cr (VI) contaminated area can cause liver injury in mice, and there may be other heavy metal pollution, while Vit C has antagonistic effect on liver injury induced by potassium dichromate.
【學位授予單位】：遼寧醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R114

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