馬尾松花粉提取物對前列腺肥大小鼠的影響
發(fā)布時間：2019-06-19 03:38
【摘要】：前列腺肥大(BPH)是老年男性的常見疾病，為患者的身體和心靈造成了很大的損害。目前治療BPH的主流藥物為5α-還原酶抑制劑和α1-腎上腺素受體阻滯劑，但它們均存在一定的副作用。馬尾松花粉提取物(MPPE)是一種具有良好的抗炎和抗氧化活性的天然植物提取物，其營養(yǎng)豐富，無毒副作用。很多研究表明，MPPE可用于改善BPH。本實驗擬采用腹腔注射丙酸睪酮（5mg/kg·d）的方法建立小鼠BPH模型，通過探究MPPE以及SQ、PGY兩種復(fù)方對小鼠BPH的影響，為松花粉進一步開發(fā)為抗BPH藥物或保健品提供理論依據(jù)。實驗包括以下幾部分： 第一，，MPPE對小鼠BPH的預(yù)防作用探究：實驗周期為6周，前兩周三個松花粉組小鼠預(yù)先灌胃相應(yīng)劑量的MPPE。后四周，在腹腔注射丙酸睪酮建立BPH模型的同時給予三種不同劑量（20、40、80mg/kg）的MPPE。實驗結(jié)束時收集小鼠尿液，進行代謝組學(xué)分析，然后處死小鼠收集血清測定相關(guān)生理指標(biāo)，剖取前列腺制作石蠟切片，觀察組織形態(tài)變化。 結(jié)果顯示，與空白組相比，模型組小鼠的前列腺重量、前列腺指數(shù)顯著增加，前列腺上皮細胞增生，向腺腔內(nèi)折疊呈絨毛狀，形態(tài)結(jié)構(gòu)具有明顯的BPH特征。三種劑量的MPPE作用效果有一定差異。與模型組相比，20mg/kg MPPE保護作用較小，而40、80mg/kgMPPE組的前列腺指數(shù)、炎性細胞因子含量、DHT濃度及PACP、NOS的活性均有明顯的降低，NO的生成也相應(yīng)減少。其中，80mg/kg的MPPE效果最好。PLS-DA結(jié)果與生化指標(biāo)基本一致，對照組與模型組小鼠完全分開，說明BPH小鼠的尿液代謝物發(fā)生了明顯的變化。非那雄胺組最接近空白組，MPPE的保護作用比較明顯，但并未使小鼠完全恢復(fù)健康。 第二，MPPE對小鼠BPH的治療作用探究：與預(yù)防作用部分實驗不同的是，治療作用實驗先腹腔注射丙酸睪酮4周建立BPH模型。然后再分別灌胃不同濃度的MPPE治療4周。結(jié)果顯示，模型組小鼠的前列腺重量、前列腺指數(shù)顯著增加，前列腺組織形態(tài)具有明顯的BPH特征。20mg/kg MPPE作用較小，80mg/kg的MPPE效果最好。BPH小鼠的尿液代謝物發(fā)生了明顯的變化，MPPE作用效果比較明顯，但并未使小鼠完全恢復(fù)健康。非那雄胺對BPH的改善效果最佳。 另外，對PI百分抑制率進行對比，預(yù)防試驗中三個劑量MPPE的抑制率分別為26.09%、52.17%、69.57%，而治療實驗的則分別為33.33%、57.14%、76.19%，可以看出MPPE對BPH的預(yù)防作用優(yōu)于其治療作用。 第三，MPPE及SQ、PGY復(fù)方對小鼠BPH的影響：實驗具體步驟與MPPE治療作用實驗一致，1-4周：建模；5-8周：治療。實驗結(jié)束后測定相關(guān)指標(biāo)。實驗結(jié)果顯示，SQ、PGY兩個復(fù)方的抗BPH活性明顯優(yōu)于MPPE。且SQ的效果比PGY稍好，但兩者差異沒有統(tǒng)計學(xué)意義。 以上結(jié)果表明，（1）MPPE對BPH的預(yù)防作用優(yōu)于其治療作用，推測可能的原因是因為一邊建模一邊給藥，MPPE及時的發(fā)揮了保護作用，減少了小鼠患BPH的可能；（2）SQ、PGY復(fù)方的效果優(yōu)于MPPE，且SQ的效果比PGY稍好。說明SQ、PGY兩種中草藥與MPPE復(fù)合后可以起到一定的強化作用，能更好的改善BPH病癥，對機體產(chǎn)生顯著的保護作用；（3）MPPE可以降低BPH病理癥狀及相關(guān)生化指標(biāo)，調(diào)節(jié)標(biāo)志性代謝物的水平，且具有一定的劑量依賴性，MPPE的抗BPH作用機理可能與其抗炎抗氧化活性有關(guān)。
[Abstract]:Prostatic hypertrophy (BPH) is a common disease in the elderly, causing great damage to the body and mind of the patient. Currently, the main drug for treating BPH is a 5-dose reductase inhibitor and a 1-epinephrine receptor blocker, but all of them have certain side effects. Pinus massoniana pollen extract (MPPE) is a natural plant extract with good anti-inflammatory and antioxidant activity, and has rich nutrition and no toxic and side effects. Many studies have shown that MPPE can be used to improve BPH. In this experiment, a mouse BPH model was established by means of intraperitoneal injection of testosterone propionate (5 mg/ kg 路 d), and the effect of two compounds of MPPE and SQ and PGY on the BPH of mice was investigated. The experiment includes the following parts: First, the effect of MPPE on the prevention of BPH in mice: the experimental period is 6 weeks, and the corresponding dose of MPP in the three pine pollen group in the first two weeks in advance E. Three different doses of MPP (20,40,80 mg/ kg) were administered at the same time of the intraperitoneal injection of testosterone propionate to establish a BPH model and E. collecting the urine of the mouse at the end of the experiment, performing metabonomics analysis, then executing the mouse to collect the relevant physiological indexes of the serum, cutting the prostate to make the paraffin section, and observing the morphological change of the tissue; The results showed that, compared with the blank group, the prostate weight and the prostate index of the model group mice were significantly increased, the prostatic epithelial cells were hyperplastic, folded in the glandular cavity, and the morphological structure had obvious BP. H. The effect of three doses of MPPE is one. Compared with the model group, the protective effect of 20 mg/ kg of MPPE is small, and the prostate index, the inflammatory cytokine content, the DHT concentration and the PACP and the NOS activity of the 40 and 80 mg/ kg MPPE group are obviously reduced, and the generation of NO also The MPPE effect of 80 mg/ kg should be reduced. The results showed that the results of PLS-DA were basically consistent with the biochemical indexes, and the control group was completely separated from the model group mice, indicating that the urine metabolites in BPH mice were obviously The protective effect of MPPE was more obvious than that in the blank group, but it did not completely change the mice. Complex health. Second, the effect of MPPE on the treatment of BPH in mice: It is different from the part of the preventive action: the treatment effect is first established by intraperitoneal injection of testosterone propionate for 4 weeks. BPH model. Then, the different concentrations of MPP were given. E-treatment for 4 weeks. The results showed that the prostate weight and the prostate index of the model group were significantly increased, and the prostate tissue morphology had obvious BPH characteristics. The MPPE of 20 mg/ kg was less and the MPP of 80 mg/ kg was less. E effect is the best. The urine metabolites in BPH mice have changed significantly, and the effect of MPPE is more obvious, but the mice are not finished. All-in-health. The change of the non-androgenic amine to the BPH In addition, the inhibition rate of three doses of MPPE in the test was 26.09%, 52.17% and 69.57%, respectively, and 33.33%, 57.14% and 76.19%, respectively, and the preventive effect of MPPE on BPH can be seen. The effect of the combination of the third, the MPPE and the SQ and the PGY on the BPH of the mice: the experimental specific steps are consistent with the experimental results of the treatment of the MPPE, and 1-4 weeks: construction Module;5-8 weeks: Treatment. Experiment The correlation index was determined after the end of the experiment. The results showed that the two compounds of SQ and PGY were anti-BPH. The performance of SQ is better than MPPE, and the effect of SQ is slightly better than that of PGY, but both The results show that (1) MPPE is superior to the therapeutic effect of MPPE in the prevention of BPH, and the possible cause is that MPPE plays a protective role in a timely manner, which reduces the possibility of the development of BPH in the mice. (2) The effect of SQ and PGY is superior to that of MPPE. the effect of the SQ is slightly better than that of the PGY, and the combination of the two Chinese herbal medicines and the MPPE of the SQ and PGY can play a certain strengthening effect, can better improve the BPH condition, and has a remarkable protective effect on the body; and (3) the MPPE can reduce the pathological symptoms of the BPH and the related biochemical indexes, regulating the level of the symbolic metabolite and has a certain dose-dependence, and the mechanism of the anti-BPH action of the MPPE may
【學(xué)位授予單位】：山東師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R151
本文編號：2502048
[Abstract]:Prostatic hypertrophy (BPH) is a common disease in the elderly, causing great damage to the body and mind of the patient. Currently, the main drug for treating BPH is a 5-dose reductase inhibitor and a 1-epinephrine receptor blocker, but all of them have certain side effects. Pinus massoniana pollen extract (MPPE) is a natural plant extract with good anti-inflammatory and antioxidant activity, and has rich nutrition and no toxic and side effects. Many studies have shown that MPPE can be used to improve BPH. In this experiment, a mouse BPH model was established by means of intraperitoneal injection of testosterone propionate (5 mg/ kg 路 d), and the effect of two compounds of MPPE and SQ and PGY on the BPH of mice was investigated. The experiment includes the following parts: First, the effect of MPPE on the prevention of BPH in mice: the experimental period is 6 weeks, and the corresponding dose of MPP in the three pine pollen group in the first two weeks in advance E. Three different doses of MPP (20,40,80 mg/ kg) were administered at the same time of the intraperitoneal injection of testosterone propionate to establish a BPH model and E. collecting the urine of the mouse at the end of the experiment, performing metabonomics analysis, then executing the mouse to collect the relevant physiological indexes of the serum, cutting the prostate to make the paraffin section, and observing the morphological change of the tissue; The results showed that, compared with the blank group, the prostate weight and the prostate index of the model group mice were significantly increased, the prostatic epithelial cells were hyperplastic, folded in the glandular cavity, and the morphological structure had obvious BP. H. The effect of three doses of MPPE is one. Compared with the model group, the protective effect of 20 mg/ kg of MPPE is small, and the prostate index, the inflammatory cytokine content, the DHT concentration and the PACP and the NOS activity of the 40 and 80 mg/ kg MPPE group are obviously reduced, and the generation of NO also The MPPE effect of 80 mg/ kg should be reduced. The results showed that the results of PLS-DA were basically consistent with the biochemical indexes, and the control group was completely separated from the model group mice, indicating that the urine metabolites in BPH mice were obviously The protective effect of MPPE was more obvious than that in the blank group, but it did not completely change the mice. Complex health. Second, the effect of MPPE on the treatment of BPH in mice: It is different from the part of the preventive action: the treatment effect is first established by intraperitoneal injection of testosterone propionate for 4 weeks. BPH model. Then, the different concentrations of MPP were given. E-treatment for 4 weeks. The results showed that the prostate weight and the prostate index of the model group were significantly increased, and the prostate tissue morphology had obvious BPH characteristics. The MPPE of 20 mg/ kg was less and the MPP of 80 mg/ kg was less. E effect is the best. The urine metabolites in BPH mice have changed significantly, and the effect of MPPE is more obvious, but the mice are not finished. All-in-health. The change of the non-androgenic amine to the BPH In addition, the inhibition rate of three doses of MPPE in the test was 26.09%, 52.17% and 69.57%, respectively, and 33.33%, 57.14% and 76.19%, respectively, and the preventive effect of MPPE on BPH can be seen. The effect of the combination of the third, the MPPE and the SQ and the PGY on the BPH of the mice: the experimental specific steps are consistent with the experimental results of the treatment of the MPPE, and 1-4 weeks: construction Module;5-8 weeks: Treatment. Experiment The correlation index was determined after the end of the experiment. The results showed that the two compounds of SQ and PGY were anti-BPH. The performance of SQ is better than MPPE, and the effect of SQ is slightly better than that of PGY, but both The results show that (1) MPPE is superior to the therapeutic effect of MPPE in the prevention of BPH, and the possible cause is that MPPE plays a protective role in a timely manner, which reduces the possibility of the development of BPH in the mice. (2) The effect of SQ and PGY is superior to that of MPPE. the effect of the SQ is slightly better than that of the PGY, and the combination of the two Chinese herbal medicines and the MPPE of the SQ and PGY can play a certain strengthening effect, can better improve the BPH condition, and has a remarkable protective effect on the body; and (3) the MPPE can reduce the pathological symptoms of the BPH and the related biochemical indexes, regulating the level of the symbolic metabolite and has a certain dose-dependence, and the mechanism of the anti-BPH action of the MPPE may
【學(xué)位授予單位】：山東師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R151
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