【摘要】：三氯乙烯（Trichloroethylene，TCE)，是一種氯代溶劑，由于其具有良好的親脂性和低熔點的物理特性，在工業(yè)生產(chǎn)中是一種理想的去油劑和干燥劑，在家庭生活中，廣泛應用于粘合劑，潤滑劑和改正液等產(chǎn)品中。但由于在生產(chǎn)生活中使用TCE后未妥善處理，使得越來越多的TCE進入環(huán)境當中，成為一種環(huán)境污染物，危害人類的健康。TCE具有多種作用，包括神經(jīng)毒性，腎損傷，肝損傷，肺損傷，心率變化，免疫器官病理變化等，然而，迄今為止，TCE對機體的毒性作用機制還不清楚。因此，TCE已被列入美國國家環(huán)保局在1976年公布的129種優(yōu)先污染物和我國環(huán)境特征污染物的“黑名單”。 核因子-κB受體活化因子配體(Receptor Activator for Nuclear Factor-κBLigand，RANKL)屬于腫瘤壞死因子家族的成員，其受體是核因子κB受體活化因子(receptor activator of NF-κB，，RANK)。RANKL具有多種作用，包括參與骨重建，淋巴結(jié)的形成，胸腺微環(huán)境的建立，懷孕過程中的乳腺發(fā)育，激素引起的乳腺癌，和癌癥過程中的骨轉(zhuǎn)移等過程。 本文通過灌胃給予TCE致大鼠心，肝，肺，腎，脾和胸腺的損傷，采用蘇木精-伊紅染色、免疫組織化學和Western-Blot方法檢測了TCE對大鼠毒性損害及RANKL的表達情況，系統(tǒng)性的研究了TCE引起的毒性損傷及RANKL之間的相關性。結(jié)果發(fā)現(xiàn)TCE能明顯的引起大鼠體重的下降，加劇各器官的組織病理學形態(tài)損傷，且能提高不同組織中RANKL的表達，表明RANKL參與了的TCE對機體損傷。此研究為環(huán)境毒素TCE的毒性及其作用機制提供理論基礎。
[Abstract]:Trichloroethylene (Trichloroethylene,TCE) is a kind of chlorine solvent. It is an ideal deoiling agent and desiccant in industrial production because of its good lipophilicity and low melting point physical properties. It is widely used in family life. Lubricant and correction liquid and other products. However, due to the lack of proper treatment after the use of TCE in production and life, more and more TCE enter the environment and become an environmental pollutant, which endangers human health. TCE has many functions, including neurotoxicity, kidney injury and liver injury. Lung injury, heart rate changes, pathological changes of immune organs, etc. However, so far, the toxic mechanism of TCE on the body is not clear. Therefore, TCE has been listed in the blacklist of 129 priority pollutants and environmental characteristic pollutants published by the National Environmental Protection Agency in 1976. Nuclear factor-kappa B receptor activating factor ligand (Receptor Activator for Nuclear Factor- 魏 BLigand, RANKL) belongs to tumor necrosis factor family. Its receptor is nuclear factor kappa B receptor activating factor (receptor activator of NF- kappa B, RANK). Rankl has many effects. It includes bone reconstruction, lymph node formation, thymic microenvironment establishment, breast development during pregnancy, hormone-induced breast cancer, and bone metastasis during cancer. The injury of heart, liver, lung, kidney, spleen and thymic gland induced by TCE was detected by hematoxylin-eosin staining, immunohistochemistry and Western-Blot. The toxic damage and the expression of RANKL in rats were detected by TCE. The correlation between toxic damage induced by TCE and RANKL was studied systematically. The results showed that TCE could significantly decrease the body weight, aggravate the pathological morphological damage of various organs, and increase the expression of RANKL in different tissues, indicating that RANKL was involved in the injury of TCE to the body. This study provides a theoretical basis for the toxicity and mechanism of environmental toxin TCE.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R114

【參考文獻】

相關期刊論文 前10條

1 王家驥,練海泉,胡明霞,晁斌,朱啟星,林麗白;三氯乙烯對職業(yè)人群的細胞遺傳學效應[J];癌變.畸變.突變;2003年01期

2 張達政,陳鴻漢,李海明,鄒勝章,劉立才;淺層地下水鹵代烴污染初步研究[J];中國地質(zhì);2002年03期

3 高存榮;王俊桃;;我國69個城市地下水有機污染特征研究[J];地球?qū)W報;2011年05期

4 黃媛媛;單愛琴;李海花;蔡靜;李玲;;三氯乙烯污染對植物、土壤生物學指標的影響[J];環(huán)境科學與技術;2010年10期

5 胡明霞;三氯乙烯的毒理學研究新進展[J];國外醫(yī)學(衛(wèi)生學分冊);2001年03期

6 解玉梅;三氯乙烯國內(nèi)外市場分析[J];化工技術經(jīng)濟;2005年04期

7 黃海燕;莊志雄;劉建軍;;三氯乙烯中毒表現(xiàn)及其作用機制研究進展[J];環(huán)境與職業(yè)醫(yī)學;2006年01期

8 劉移民;艾寶民;王致;;我國三氯乙烯職業(yè)危害研究十年回顧[J];中國工業(yè)醫(yī)學雜志;2007年02期

9 劉移民,艾玲保,肖勇梅,林炳杰,楊柏兵,招小林,庚永沖,周光宇;三氯乙烯對接觸工人神經(jīng)行為功能影響的初步研究[J];中國職業(yè)醫(yī)學;2001年04期

10 李來玉,冷曙光,鄭玉新,何家禧,黃建勛,何鳳生;三氯乙烯藥疹樣皮炎代謝酶基因多態(tài)性的病例對照研究[J];中國職業(yè)醫(yī)學;2002年03期

本文編號：2495689