【摘要】：綠色、原位、快速、無損檢測技術(shù)的開發(fā)是分析行業(yè)新的發(fā)展方向。近年來常壓快速質(zhì)譜技術(shù)以突破傳統(tǒng)質(zhì)譜分析需冗長預(yù)處理和分離的限制,及實現(xiàn)樣品的原位、實時、在線、非破壞和低耗損的質(zhì)譜分析,得以迅速發(fā)展。表面解吸常壓化學(xué)電離質(zhì)譜(DAPCI-MS)技術(shù)是新興的常壓快速質(zhì)譜技術(shù)之一,可在常溫、常壓、無解吸溶劑的條件下對未經(jīng)樣品預(yù)處理的各種復(fù)雜樣品表面痕量物質(zhì)進行解吸電離,對樣品無污染、無破壞,是對實際樣品原位質(zhì)譜檢測較為理想的電離方法之一。本文主要研究DAPCI-MS在原料肉品質(zhì)及齲齒表面微區(qū)分析中的應(yīng)用。本文的主要研究內(nèi)容包括:1.DAPCI-MS技術(shù)在豬肉新鮮度評價中的應(yīng)用。在無需樣品預(yù)處理的條件下,采用DAPCI-MS方法直接獲取豬肉新鮮度隨時間變化的質(zhì)譜指紋譜圖,采用串聯(lián)質(zhì)譜技術(shù)對關(guān)鍵目標化合物進行了初步的分子式鑒定,并結(jié)合PCA和CA方法,分別對不同新鮮度的原料肉進行區(qū)分及方法的可重現(xiàn)性分析。結(jié)果表明:冷藏保存1~8天豬肉的質(zhì)譜指紋譜圖明顯分為三類,第一類為1~3天的質(zhì)譜圖,是新鮮度變化的初期,第二類為4~6天的質(zhì)譜圖,是新鮮度向腐敗變化的過渡時期,第三類為7~8天的質(zhì)譜圖,是品質(zhì)完全發(fā)生變化的時期。該方法簡單、快速,是分析復(fù)雜基體樣品的一種有效實用的分析手段。2.DAPCI-MS技術(shù)在原料肉摻假分析中的應(yīng)用。選擇4種原料肉和3種非同源摻假肉進行DAPCI-MS分析,結(jié)合PCA方法,對真假肉進行區(qū)分;采用未知真?zhèn)蔚娜庠礃悠愤M行驗證,結(jié)果匹配。該方法對基體復(fù)雜的肉類樣品具有較好的分析能力,單個樣品的分析時間不超過30 s,原料肉和摻假肉的質(zhì)譜指紋譜圖之間存在差異,結(jié)合主成分分析(PCA)法能有效地將原料肉和摻假肉區(qū)分開。3.齲齒表面微區(qū)的DAPCI-MS分析。采用納升取樣DAPCI-MS直接對齲齒不同部位取樣并進行快速質(zhì)譜分析,結(jié)合PCA方法,對齲齒病灶位置與鄰近正常組織進行區(qū)分,以及牙病與健康牙齒的區(qū)分;結(jié)合串聯(lián)質(zhì)譜可快速檢定齲齒中的乳酸、丙酮酸、苯乙酸和丙酸等關(guān)鍵成分。該方法及裝置可方便地對牙齒進行直接微區(qū)分析,為鑒別牙齒疾病、觀測治療效果提供了一種快速、簡單的方法,為生物體中微細部位的快速取樣及直接質(zhì)譜分析提供了一種可能的解決方案。
[Abstract]:The development of green, in situ, fast and nondestructive testing technology is a new development direction of analysis industry. In recent years, atmospheric pressure rapid mass spectrometry has been developed rapidly to break through the limitation of long pretreatment and separation of traditional mass spectrometry, and to realize in situ, real-time, on-line, non-destructive and low loss mass spectrometry of samples. Surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS) is one of the new atmospheric pressure rapid mass spectrometry techniques, which can be used at room temperature and atmospheric pressure. Desorption and ionization of trace substances on the surface of various complex samples without desorption solvent is one of the ideal ionization methods for in situ mass spectrometry of actual samples. In this paper, the application of DAPCI-MS in raw meat quality and dental caries surface microanalysis was studied. The main research contents of this paper include: the application of 1.DAPCI-MS technology in pork freshness evaluation. Under the condition of no sample pretreatment, the mass spectrometry fingerprint of pork freshness changed with time was obtained by DAPCI-MS method, and the key target compounds were identified by tandem mass spectrometry. Combined with PCA and CA methods, the raw meat with different freshness was distinguished and the reproducibility of the method was analyzed. The results showed that the mass spectrometry fingerprints of pork stored in cold storage for 1 脳 8 days were obviously divided into three categories. The first type was 1 脳 3 days mass spectrometry, which was the initial stage of freshness change, and the second type was 4 脳 6 days mass spectrometry, which was the transitional period from freshness to corruption. The third type is 7 脳 8 days mass spectrometry, which is a period of complete quality change. This method is simple and rapid, and it is an effective and practical method for the analysis of complex matrix samples. Application of 2.DAPCI-MS technique in adulteration Analysis of Raw Meat. Four kinds of raw meat and three kinds of non-homologous adulterated meat were selected for DAPCI-MS analysis, combined with PCA method, the true and false meat was distinguished, and the unknown true and false meat source samples were used to verify the results, and the results were matched. This method has good analytical ability for meat samples with complex matrix. The analysis time of a single sample is not more than 30 s. There is a difference in mass spectrometry fingerprint between raw meat and adulterated meat. Combined with principal component analysis (PCA), (PCA) can effectively distinguish raw meat from adulterated meat. DAPCI-MS analysis of microarea on the surface of caries. DAPCI-MS was used to directly sample different parts of dental caries and rapid mass spectrometry analysis was carried out. Combined with PCA method, the location of caries lesions was distinguished from adjacent normal tissues, as well as the distinction between dental diseases and healthy teeth. Lactic acid, pyruvate, phenylacetic acid and propionic acid in dental caries can be quickly identified by tandem mass spectrometry. The method and device can easily analyze the teeth directly, which provides a rapid and simple method for distinguishing dental diseases and observing the therapeutic effect. It provides a possible solution for rapid sampling and direct mass spectrometry analysis of fine parts in organisms.
【學(xué)位授予單位】：東華理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R155.5;TS207.3;O657.63

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本文編號：2485375