發(fā)布時(shí)間：2019-05-25 01:35

【摘要】：綠色、原位、快速、無(wú)損檢測(cè)技術(shù)的開(kāi)發(fā)是分析行業(yè)新的發(fā)展方向。近年來(lái)常壓快速質(zhì)譜技術(shù)以突破傳統(tǒng)質(zhì)譜分析需冗長(zhǎng)預(yù)處理和分離的限制,及實(shí)現(xiàn)樣品的原位、實(shí)時(shí)、在線、非破壞和低耗損的質(zhì)譜分析,得以迅速發(fā)展。表面解吸常壓化學(xué)電離質(zhì)譜(DAPCI-MS)技術(shù)是新興的常壓快速質(zhì)譜技術(shù)之一,可在常溫、常壓、無(wú)解吸溶劑的條件下對(duì)未經(jīng)樣品預(yù)處理的各種復(fù)雜樣品表面痕量物質(zhì)進(jìn)行解吸電離,對(duì)樣品無(wú)污染、無(wú)破壞,是對(duì)實(shí)際樣品原位質(zhì)譜檢測(cè)較為理想的電離方法之一。本文主要研究DAPCI-MS在原料肉品質(zhì)及齲齒表面微區(qū)分析中的應(yīng)用。本文的主要研究?jī)?nèi)容包括:1.DAPCI-MS技術(shù)在豬肉新鮮度評(píng)價(jià)中的應(yīng)用。在無(wú)需樣品預(yù)處理的條件下,采用DAPCI-MS方法直接獲取豬肉新鮮度隨時(shí)間變化的質(zhì)譜指紋譜圖,采用串聯(lián)質(zhì)譜技術(shù)對(duì)關(guān)鍵目標(biāo)化合物進(jìn)行了初步的分子式鑒定,并結(jié)合PCA和CA方法,分別對(duì)不同新鮮度的原料肉進(jìn)行區(qū)分及方法的可重現(xiàn)性分析。結(jié)果表明:冷藏保存1~8天豬肉的質(zhì)譜指紋譜圖明顯分為三類,第一類為1~3天的質(zhì)譜圖,是新鮮度變化的初期,第二類為4~6天的質(zhì)譜圖,是新鮮度向腐敗變化的過(guò)渡時(shí)期,第三類為7~8天的質(zhì)譜圖,是品質(zhì)完全發(fā)生變化的時(shí)期。該方法簡(jiǎn)單、快速,是分析復(fù)雜基體樣品的一種有效實(shí)用的分析手段。2.DAPCI-MS技術(shù)在原料肉摻假分析中的應(yīng)用。選擇4種原料肉和3種非同源摻假肉進(jìn)行DAPCI-MS分析,結(jié)合PCA方法,對(duì)真假肉進(jìn)行區(qū)分;采用未知真?zhèn)蔚娜庠礃悠愤M(jìn)行驗(yàn)證,結(jié)果匹配。該方法對(duì)基體復(fù)雜的肉類樣品具有較好的分析能力,單個(gè)樣品的分析時(shí)間不超過(guò)30 s,原料肉和摻假肉的質(zhì)譜指紋譜圖之間存在差異,結(jié)合主成分分析(PCA)法能有效地將原料肉和摻假肉區(qū)分開(kāi)。3.齲齒表面微區(qū)的DAPCI-MS分析。采用納升取樣DAPCI-MS直接對(duì)齲齒不同部位取樣并進(jìn)行快速質(zhì)譜分析,結(jié)合PCA方法,對(duì)齲齒病灶位置與鄰近正常組織進(jìn)行區(qū)分,以及牙病與健康牙齒的區(qū)分;結(jié)合串聯(lián)質(zhì)譜可快速檢定齲齒中的乳酸、丙酮酸、苯乙酸和丙酸等關(guān)鍵成分。該方法及裝置可方便地對(duì)牙齒進(jìn)行直接微區(qū)分析,為鑒別牙齒疾病、觀測(cè)治療效果提供了一種快速、簡(jiǎn)單的方法,為生物體中微細(xì)部位的快速取樣及直接質(zhì)譜分析提供了一種可能的解決方案。
[Abstract]:The development of green, in situ, fast and nondestructive testing technology is a new development direction of analysis industry. In recent years, atmospheric pressure rapid mass spectrometry has been developed rapidly to break through the limitation of long pretreatment and separation of traditional mass spectrometry, and to realize in situ, real-time, on-line, non-destructive and low loss mass spectrometry of samples. Surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS) is one of the new atmospheric pressure rapid mass spectrometry techniques, which can be used at room temperature and atmospheric pressure. Desorption and ionization of trace substances on the surface of various complex samples without desorption solvent is one of the ideal ionization methods for in situ mass spectrometry of actual samples. In this paper, the application of DAPCI-MS in raw meat quality and dental caries surface microanalysis was studied. The main research contents of this paper include: the application of 1.DAPCI-MS technology in pork freshness evaluation. Under the condition of no sample pretreatment, the mass spectrometry fingerprint of pork freshness changed with time was obtained by DAPCI-MS method, and the key target compounds were identified by tandem mass spectrometry. Combined with PCA and CA methods, the raw meat with different freshness was distinguished and the reproducibility of the method was analyzed. The results showed that the mass spectrometry fingerprints of pork stored in cold storage for 1 脳 8 days were obviously divided into three categories. The first type was 1 脳 3 days mass spectrometry, which was the initial stage of freshness change, and the second type was 4 脳 6 days mass spectrometry, which was the transitional period from freshness to corruption. The third type is 7 脳 8 days mass spectrometry, which is a period of complete quality change. This method is simple and rapid, and it is an effective and practical method for the analysis of complex matrix samples. Application of 2.DAPCI-MS technique in adulteration Analysis of Raw Meat. Four kinds of raw meat and three kinds of non-homologous adulterated meat were selected for DAPCI-MS analysis, combined with PCA method, the true and false meat was distinguished, and the unknown true and false meat source samples were used to verify the results, and the results were matched. This method has good analytical ability for meat samples with complex matrix. The analysis time of a single sample is not more than 30 s. There is a difference in mass spectrometry fingerprint between raw meat and adulterated meat. Combined with principal component analysis (PCA), (PCA) can effectively distinguish raw meat from adulterated meat. DAPCI-MS analysis of microarea on the surface of caries. DAPCI-MS was used to directly sample different parts of dental caries and rapid mass spectrometry analysis was carried out. Combined with PCA method, the location of caries lesions was distinguished from adjacent normal tissues, as well as the distinction between dental diseases and healthy teeth. Lactic acid, pyruvate, phenylacetic acid and propionic acid in dental caries can be quickly identified by tandem mass spectrometry. The method and device can easily analyze the teeth directly, which provides a rapid and simple method for distinguishing dental diseases and observing the therapeutic effect. It provides a possible solution for rapid sampling and direct mass spectrometry analysis of fine parts in organisms.
【學(xué)位授予單位】：東華理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R155.5;TS207.3;O657.63

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 李秀杰;滕國(guó)玲;余紅;;基于聚類分析的消費(fèi)者綠色羊肉購(gòu)買行為研究[J];黑龍江畜牧獸醫(yī);2014年11期

2 陸昌華;胡肄農(nóng);甘泉;孫友珍;譚業(yè)平;郁達(dá)威;劉亮;田景福;儲(chǔ)新民;;動(dòng)物食品質(zhì)量安全風(fēng)險(xiǎn)因素分析及其防控對(duì)策[J];江蘇農(nóng)業(yè)學(xué)報(bào);2015年03期

相關(guān)博士學(xué)位論文 前3條

1 孫洪波;豬肉供應(yīng)鏈上的機(jī)會(huì)主義根源及治理對(duì)策研究[D];中國(guó)農(nóng)業(yè)科學(xué)院;2012年

2 費(fèi)亞利;政府強(qiáng)制性豬肉質(zhì)量安全可追溯體系研究[D];四川農(nóng)業(yè)大學(xué);2012年

3 劉萬(wàn)兆;基于封閉供應(yīng)鏈的豬肉質(zhì)量安全控制研究[D];沈陽(yáng)農(nóng)業(yè)大學(xué);2013年

相關(guān)碩士學(xué)位論文 前1條

1 沈斌;大部制背景下我國(guó)豬肉類產(chǎn)品安全監(jiān)管體系之完善研究[D];南昌大學(xué);2015年

，

本文編號(hào)：2485375