【摘要】：從黃乳粘蓋牛肝菌(Suillus flavus)中分離得到的異牛肝菌素(iso-suillin)具有顯著的抗腫瘤活性,但其在心血管系統(tǒng)的功效尚不明確。本研究用不同濃度的異牛肝菌素預(yù)處理原代血管平滑肌細(xì)胞(vascular smooth muscle cell,VSMC)24 h或48 h后,再采用血小板源性生長(zhǎng)因子(platelet-derived growth factor,PDGF)-BB處理細(xì)胞,細(xì)胞計(jì)數(shù)法檢測(cè)VSMC增殖活性;流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期分布;Western blot分析檢測(cè)蛋白表達(dá)情況;免疫沉淀檢測(cè)平滑肌22 alpha(smooth muscle 22 alpha,SM22α)磷酸化和泛素化水平。結(jié)果顯示,異牛肝菌素顯著抑制PDGF-BB引發(fā)的VSMC增殖;上調(diào)分化標(biāo)志蛋白SM22α表達(dá),下調(diào)增殖相關(guān)蛋白PCNA表達(dá);抑制SM22α磷酸化及其泛素化降解,且該過(guò)程可能與其阻斷蛋白激酶C(protein kinase C,PKC)δ的活性有關(guān)。通過(guò)以上結(jié)果推測(cè),異牛肝菌素通過(guò)抑制PKCδ的活性,降低SM22α磷酸化水平,阻止SM22α泛素化降解,進(jìn)而抑制VSMC表型轉(zhuǎn)化,發(fā)揮血管保護(hù)的功能。
[Abstract]:Isoborubicin (iso-suillin) isolated from (Suillus flavus) has significant antitumor activity, but its effect on cardiovascular system is not clear. In this study, primary vascular smooth muscle cells (vascular smooth muscle cell,VSMC) were pretreated with different concentrations of isoborubicin for 24 h or 48 h, and then treated with platelet-derived growth factor (platelet-derived growth factor,PDGF)-BB. The proliferation activity of VSMC was detected by cell counting. Cell cycle distribution was detected by flow cytometry, protein expression was detected by; Western blot analysis, and phosphorylation and ubiquitin levels of smooth muscle 22 alpha (smooth muscle 22 alpha,SM22 偽 were detected by immunoprecipitation. The results showed that genistein significantly inhibited the proliferation of VSMC induced by PDGF-BB, upregulated the expression of differentiation marker protein SM22 偽 and down-regulated the expression of proliferation-related protein PCNA. Inhibition of SM22 偽 phosphorylation and ubiquitin degradation may be related to its blocking the activity of protein kinase C (protein kinase C, PKC) 未. Through the above results, it is speculated that isoborubicin can inhibit the activity of PKC 未, decrease the phosphorylation level of SM22 偽, prevent the degradation of SM22 偽 ubiquitin, and then inhibit the phenotypic transformation of VSMC and play the role of vascular protection.
【作者單位】： 河北醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院;河北省食品檢驗(yàn)研究院河北省食品安全重點(diǎn)實(shí)驗(yàn)室;北京食品科學(xué)研究院中國(guó)肉類(lèi)食品綜合研究中心;
【基金】：“十三五”國(guó)家重點(diǎn)研發(fā)計(jì)劃重點(diǎn)專(zhuān)項(xiàng)(2016YFF0202300) 科技部質(zhì)檢行業(yè)公益專(zhuān)項(xiàng)(201510208-08) 國(guó)家自然科學(xué)基金青年科學(xué)基金項(xiàng)目(81300225) 河北省自然科學(xué)基金資助項(xiàng)目(H2015206467) 河北省高等學(xué)校優(yōu)秀青年基金項(xiàng)目(YQ2013018)
【分類(lèi)號(hào)】：R151

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