發(fā)布時(shí)間：2019-04-26 12:42

【摘要】：目的:探討錳染毒對SH-SY5Y細(xì)胞線粒體超微結(jié)構(gòu)的影響及線粒體功能的改變。方法:透射電鏡觀察對照組和MnCl_2處理組(250μmol/L,6 h)SH-SY5Y細(xì)胞線粒體形態(tài)的變化,流式細(xì)胞術(shù)檢測細(xì)胞膜電位變化,以及熒光酶標(biāo)儀檢測細(xì)胞內(nèi)活性氧的含量。結(jié)果:與對照組相比,MnCl_2處理組線粒體形態(tài)發(fā)生明顯的腫脹、空泡化、基質(zhì)濃縮明顯,線粒體嵴數(shù)減少或消失;細(xì)胞線粒體膜電位在250μmol/L作用6 h后明顯降低(P0.05);同時(shí)在相同處理?xiàng)l件下,ROS產(chǎn)生水平較對照組有明顯升高(P0.05)。結(jié)論:錳可以損傷線粒體的超微結(jié)構(gòu)并影響其功能。
[Abstract]:Aim: to investigate the effect of manganese exposure on mitochondrial ultrastructure and mitochondrial function in SH-SY5Y cells. Methods: the mitochondrial morphology of SH-SY5Y cells was observed by transmission electron microscopy (TEM), the changes of cell membrane potential by flow cytometry and the content of reactive oxygen species (Ros) in SH-SY5Y cells treated with MnCl_2 (250 渭 mol / L, 6 h). Results: compared with the control group, the mitochondria of MnCl_2 treated group had obvious swelling, vacuolization and matrix concentration, and the number of mitochondria cristae decreased or disappeared. The mitochondrial membrane potential was significantly decreased at 250 渭 mol / L for 6 h (P0.05), and the level of ROS production was significantly higher than that of the control group under the same treatment (P0.05). Conclusion: manganese can damage the ultrastructure of mitochondria and affect its function.
【作者單位】： 首都醫(yī)科大學(xué)公共衛(wèi)生學(xué)院環(huán)境毒理學(xué)北京市重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(編號:81673137) 北京市自然科學(xué)基金項(xiàng)目(編號:7172024) 北京市教育委員會(huì)高等學(xué)校高層次人才引進(jìn)與培養(yǎng)及創(chuàng)新團(tuán)隊(duì)建設(shè)計(jì)劃項(xiàng)目(編號:CIT&TCD201504094)
【分類號】：R114

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