【摘要】：目的探討鄰苯二甲酸二乙基己酯(DEHP)對青春期雄性大鼠睪丸及性激素的影響及潛在機(jī)制。方法 18日齡SD大鼠24只,按體重隨機(jī)分為4組(對照組、低、中、高劑量DEHP組),每組6只。DEHP染毒劑量分別為250、500和750 mg/kg,經(jīng)口連續(xù)染毒30 d;頸部脫臼處死,收集血清,分離睪丸組織;測定血清睪酮、黃體生成素(LH)和卵泡刺激素(FSH)等性激素水平及睪丸組織中谷胱甘肽過氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛和5α-還原酶(5α-R)活性。結(jié)果 DEHP處理組,大鼠睪丸和附睪臟器系數(shù)降低,睪丸曲精小管管腔縮小,結(jié)構(gòu)坍塌,部分細(xì)胞膜破裂,細(xì)胞排列紊亂;與對照組比較,高劑量DEHP組大鼠血清睪酮和LH水平[分別為(115.04±14.21)pg/m L、(23.42±3.12)m IU/m L]降低,中、高劑量DEHP組大鼠血清FSH水平[分別為(5.49±0.42)、(4.63±0.51)IU/L]下降,差異有統(tǒng)計(jì)學(xué)意義(P0.05);與對照組比較,高劑量DEHP組大鼠睪丸組織中GSH-Px和SOD活力[分別為(728.3±95.3)、(835.8±151.2)U/mg pro]降低,而中、高劑量DEHP組大鼠睪丸組織中丙二醛含量[分別為(79.6±13.9)、(90.6±4.0)nmol/mg pro]和5α-R2活性[分別為(415.9±112.7)、(415.9±147.8)IU/mg pro]升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 DEHP暴露后,大鼠睪丸發(fā)生氧化應(yīng)激,并通過誘導(dǎo)5α-R2活性降低血清睪酮水平。
[Abstract]:Objective to investigate the effects of diethylhexyl phthalate (DEHP) on testis and sex hormones in adolescent male rats and its potential mechanism. Methods Twenty-four 18-day-old SD rats were randomly divided into 4 groups according to body weight (control group, low, middle and high dose DEHP group), 6 rats in each group. The doses of DEHP were 250500 and 750mg/kg, respectively for 30 days. The dislocated neck was executed, serum was collected and testicular tissue was isolated. The levels of testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD),) in testis were measured. Malondialdehyde (MDA) and 5 偽-reductase (5 偽-R) activity. Results in DEHP treated group, the organ coefficients of testis and epididymis were decreased, the tubule of testicular seminiferous tubule was reduced, the structure collapsed, some cell membranes were broken and the arrangement of cells was disordered. Compared with the control group, the serum testosterone and LH levels in the high-dose DEHP group [(115.04 鹵14.21) pg/m / L, (23.42 鹵3.12) m IU/m / L] were significantly lower than those in the control group (P < 0.05). The level of serum FSH in high dose DEHP group [(5.49 鹵0.42), (4.63 鹵0.51) IU/L] decreased significantly (P0.05). Compared with the control group, the activities of GSH-Px and SOD [(728.3 鹵95.3), (835.8 鹵151.2) U/mg pro] in the testis of the high dose DEHP group were decreased, while the activity of the SOD in the testis of the high dose group was lower than that in the control group. In high dose DEHP group, MDA content [(79.6 鹵13.9), (, 90.6 鹵4.0) nmol/mg pro] and 5 偽-R2 activity [(415.9 鹵112.7), (415.9 鹵147.8) IU/mg pro] increased, respectively. The difference was statistically significant (P0.05). Conclusion oxidative stress occurs in the testis of rats exposed to DEHP, and the serum testosterone level is decreased by inducing the activity of 5 偽-R2.
【作者單位】： 重慶市人口和計(jì)劃生育科學(xué)技術(shù)研究院出生缺陷與生殖健康重點(diǎn)實(shí)驗(yàn)室;
【基金】：重慶市科委基金(CStc2015jcyj A10004)
【分類號】：R114

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