發(fā)布時(shí)間：2019-03-25 11:46

【摘要】：化學(xué)發(fā)光(Chemiluminescence, CL)檢測法以其簡單、成本低、分析速度快、靈敏度高等優(yōu)點(diǎn)成為一種實(shí)用的微量及痕量分析方法。近年來，，發(fā)光試劑和體系不斷得到改進(jìn)與完善。隨著新體系的開發(fā)以及與其它技術(shù)的聯(lián)用，基于化學(xué)發(fā)光的流動(dòng)注射技術(shù)和毛細(xì)管電泳的新方法以其快速、簡便、靈敏等優(yōu)點(diǎn)在衛(wèi)生檢驗(yàn)領(lǐng)域得到廣泛應(yīng)用。 本實(shí)驗(yàn)將化學(xué)發(fā)光檢測技術(shù)與流動(dòng)注射相結(jié)合，建立了測定注射液中鹽酸罌粟堿的流動(dòng)注射化學(xué)發(fā)光檢測新方法；將魯米諾-三價(jià)銀(Ag(III))絡(luò)合物化學(xué)發(fā)光新體系與毛細(xì)管電泳分離技術(shù)聯(lián)用，建立了尿樣中萘酚的分離及血清中精氨酸的分離新方法。 一、流動(dòng)注射化學(xué)發(fā)光法測定鹽酸罌粟堿注射液中的鹽酸罌粟堿 目的：建立測定鹽酸罌粟堿的流動(dòng)注射化學(xué)發(fā)光新方法。 方法：堿性介質(zhì)中,鹽酸罌粟堿對(duì)魯米諾-鐵氰化鉀化學(xué)發(fā)光體系有顯著增強(qiáng)的作用，且在一定范圍內(nèi)，鹽酸罌粟堿的濃度與化學(xué)發(fā)光強(qiáng)度成正比�；诖�，本實(shí)驗(yàn)建立了流動(dòng)注射-化學(xué)發(fā)光測定鹽酸罌粟堿注射液中鹽酸罌粟堿的方法，并研究了影響化學(xué)發(fā)光強(qiáng)度的因素。 結(jié)果：在最優(yōu)條件下，測得最低檢出限為1.70μg/mL，線性范圍為5.00-200.00μg/mL (r=0.999)，平均回收率為101.30%。 結(jié)論：該方法已用于鹽酸罌粟堿注射液的測定，結(jié)果準(zhǔn)確可靠。 二、毛細(xì)管電泳-化學(xué)發(fā)光法測定尿樣中的1-萘酚和2-萘酚 目的：建立測定1-萘酚和2-萘酚的毛細(xì)管電泳化學(xué)發(fā)光新方法。 方法：研究發(fā)現(xiàn)，在堿性介質(zhì)中，1-萘酚和2-萘酚對(duì)魯米諾-Ag(III)絡(luò)合物體系的化學(xué)發(fā)光有抑制作用。根據(jù)這一特點(diǎn)，建立了靈敏、快速的檢測1-萘酚和2-萘酚的方法。通過對(duì)實(shí)驗(yàn)條件的優(yōu)化，繪制標(biāo)準(zhǔn)曲線，將尿樣處理后進(jìn)行測定。 結(jié)果：在最優(yōu)條件下，1-萘酚和2-萘酚的最低檢出限分別為2.00×10~(-7)和6.00×10-6mol/L，相對(duì)標(biāo)準(zhǔn)偏差在2.03%-4.16%之間，平均加標(biāo)回收率分別為101.50%和99.30%。 結(jié)論：該方法成功分離并測定實(shí)際尿樣中的1-萘酚和2-萘酚，結(jié)果滿意。 三、毛細(xì)管電泳-化學(xué)發(fā)光法測定血樣中的精氨酸 目的：建立測定精氨酸的毛細(xì)管電泳化學(xué)發(fā)光新方法。 方法：在堿性介質(zhì)中，精氨酸對(duì)魯米諾-Ag(III)絡(luò)合物體系的化學(xué)發(fā)光有增強(qiáng)作用。根據(jù)這一特點(diǎn)，建立了快速檢測精氨酸的方法。通過對(duì)實(shí)驗(yàn)條件進(jìn)行優(yōu)化，繪制標(biāo)準(zhǔn)曲線，將血樣處理后進(jìn)行測定。 結(jié)果：在最優(yōu)條件下，精氨酸的最低檢出限為2.50×10~(-5)mol/L，相對(duì)標(biāo)準(zhǔn)偏差為1.75%，加標(biāo)回收率為96.83%。 結(jié)論：該方法簡單、快速，成功地用于血樣中的精氨酸含量的檢測。
[Abstract]:Chemiluminescent (Chemiluminescence, CL) detection has become a practical method for trace and trace analysis because of its advantages of simplicity, low cost, fast analysis and high sensitivity. In recent years, luminescent reagents and systems have been continuously improved and perfected. With the development of the new system and the combination with other technologies, the chemiluminescence-based flow injection technique and the new method of capillary electrophoresis have been widely used in the field of health examination for their advantages of fast, simple and sensitive. A new flow injection chemiluminescence detection method for the determination of papaverine hydrochloride in injection was established by combining chemiluminescence detection with flow injection. A new method for separation of naphthol from urine and separation of arginine from serum by using a new chemiluminescence system of luminol-trivalent silver (Ag (III) complex and capillary electrophoresis was established. 1. Flow injection chemiluminescence determination of papaverine hydrochloride in papaverine hydrochloride injection objective: to establish a new flow injection chemiluminescence method for the determination of papaverine hydrochloride. Methods: papaverine hydrochloride significantly enhanced the chemiluminescence system of luminol-potassium ferricyanide in alkaline medium, and the concentration of papaverine hydrochloride was proportional to the intensity of chemiluminescence in a certain range. Based on this, a flow injection-chemiluminescence method for the determination of papaverine hydrochloride in papaverine hydrochloride injection was established, and the factors affecting the intensity of chemiluminescence were studied. Results: under the optimum conditions, the detection limit was 1.70 渭 g / mL, the linear range was 5.00-2000.00 渭 g / mL (r = 0.999), and the average recovery was 101.30%. Conclusion: the method has been applied to the determination of papaverine hydrochloride injection with accurate and reliable results. Determination of 1-naphthol and 2-naphthol in urine by capillary electrophoresis-chemiluminescence method objective: to establish a new capillary electrophoresis chemiluminescence method for the determination of 1-naphthol and 2-naphthol. Methods: it was found that in alkaline medium, 1-naphthol and 2-naphthol inhibited chemiluminescence of Lumino-Ag (III) complex system. A sensitive and rapid method for the determination of 1-naphthol and 2-naphthol was established. By optimizing the experimental conditions, the standard curve was drawn, and the urine samples were measured after treatment. Results: under the optimum conditions, the detection limits of 1-naphthol and 2-naphthol were 2.00 脳 10 ~ (- 7) and 6.00 脳 10 ~ (- 6) mol / L, respectively. The relative standard deviation (RSD) was 2.03% ~ 4.16%. The average recoveries were 101.50% and 99.30%, respectively. Conclusion: the method is satisfactory for the separation and determination of 1-naphthol and 2-naphthol in actual urine samples. 3. Determination of Arginine in Blood samples by Capillary Electrophoresis-Chemiluminescence method objective: to establish a new chemiluminescence method for determination of arginine by capillary electrophoresis. Methods: arginine enhanced chemiluminescence of Lumino-Ag (III) complex system in alkaline medium. According to this characteristic, a rapid method for the determination of arginine was established. The experimental conditions were optimized, the standard curve was drawn, and the blood samples were measured. Results: under the optimum conditions, the detection limit of arginine was 2.50 脳 10 ~ (- 5) mol/L, relative standard deviation was 1.75%, and the recovery rate was 96.83%. Conclusion: the method is simple, rapid and successful for the determination of arginine in blood samples.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R114

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本文編號(hào)：2446942