花生粕發(fā)酵物對動物的延緩衰老及改善腹瀉效果研究
發(fā)布時間：2019-03-16 23:00
【摘要】：本試驗以花生粕為主要原料,利用納豆芽孢桿菌固態(tài)發(fā)酵花生粕,探究花生粕納豆菌發(fā)酵提取液,延緩雌性大鼠生殖系統(tǒng)衰老以及改善抗生素誘導型小鼠腹瀉的作用。實驗一:花生粕發(fā)酵物延緩雌鼠生殖系統(tǒng)衰老的作用研究建立雌性大鼠衰老模型,將72只雌性SD大鼠,按體重隨機區(qū)組分為:空白對照組N(生理鹽水)、模型組M(生理鹽水)、低劑量組FL(發(fā)酵物25倍稀釋液)、中劑量組FM(發(fā)酵物5倍稀釋液)、高劑量組FH(發(fā)酵物原液)和陽性對照組Y(VE100 mg/kg),每組12只。除空白對照組腹腔注射生理鹽水外,其余各組均腹腔注射D-半乳糖(400 mg/kg.d),灌胃劑量按照0.2 ml/10 g.d。實驗進行8周后,處死動物,計算臟器指數(shù),并對樣本進行組織學觀察和性激素等指標測定。結(jié)果顯示,FM組和Y組的子宮指數(shù)與卵巢指數(shù)均高于M組(P0.05)。FM組、FH組血清雌二醇(E2)含量顯著高于M組(P0.05)。FM組、FH組、Y組血清促卵泡生成素(FSH)含量顯著低于M組(P0.05)。各處理組血清促黃體生成素(LH)含量均顯著低于M組,且有統(tǒng)計學意義(P0.05)。結(jié)論:花生粕納豆菌發(fā)酵物具有抗氧化作用,中、高劑量的花生粕發(fā)酵物能延緩雌性大鼠生殖系統(tǒng)衰老。實驗二:花生粕發(fā)酵物改善小鼠腹瀉的作用研究將40只昆明小鼠按照體重隨機區(qū)組分為空白對照組A(生理鹽水)、模型組B(氨芐西林)、自然恢復組C(氨芐西林+生理鹽水)和HFY組D(氨芐西林+花生粕發(fā)酵液)。除空白對照組(A)外,各處理組均每天灌胃氨芐西林(劑量按2 mg/g bw·d,相當于成人劑量的5倍),直至各組出現(xiàn)腹瀉,造模結(jié)束,處死模型組(B)小鼠并收集腸道內(nèi)容物�？瞻讓φ战M(A)和自然恢復組(C)灌胃生理鹽水,HFY組(D)灌胃發(fā)酵原液,劑量0.2 ml/10 g.d,連續(xù)灌胃5天處死小鼠,收集腸道內(nèi)容物。依托Illumina Hi Seq2500測序平臺,對腸道內(nèi)容物進行高通量測序,探究腸道內(nèi)容物中菌群的分類及豐度。結(jié)果顯示,在門的水平上,各實驗組排在前三位的物種為擬桿菌門、厚壁菌門和變形菌門,其中擬桿菌門相對含量依次為B組(79.87%)、C組(65.90%)、A組(58.22%)和D組(45.10%)。厚壁菌門各組由高到低的順序為D、A、C、B。變形菌門的相對含量由高到低依次為B組(17.73%)、C組(17.60%)、A組(11.63%)和D組(6.92%)。在屬的水平上,B組除志賀菌屬外,其他均呈現(xiàn)較低的水平。D組毛螺菌科NK4A136屬和羅斯氏菌均高于其他各組;梭菌屬D組高于B組;糞球菌屬A、D兩組均高于B組;乳桿菌屬D組高于A、B兩組;腸球菌屬D組高于A組;多樣性指數(shù)的組間差異顯示,B組與各組都存在菌種豐度的差異(P0.05),D組的物種豐度高于其他三組(P0.05)。該結(jié)果表明花生粕發(fā)酵物對抗生素誘導腹瀉小鼠的腸道菌群有一定的調(diào)節(jié)作用,可增加腹瀉小鼠腸道有益菌的數(shù)量,抑制有害菌的生長,增加腸道菌群的豐度和物種均勻度。
[Abstract]:In this study, peanut meal was used as the main raw material for solid fermentation of peanut meal by Bacillus natto. The effects of fermentation extract of natto peanut meal on delaying senescence of reproductive system in female rats and improving antibiotic-induced diarrhea in mice were investigated. Experiment one: the effect of peanut meal ferment on delaying the senescence of reproductive system in female rats; 72 female SD rats were randomly divided into blank control group N (normal saline) according to body weight and 72 female rats were randomly divided into two groups: control group N (NS), control group N (NS), Model group M (saline), low dose group FL (fermentation material 25 times diluent), medium dose group FM (fermentation material 5 times dilution liquid), high dose group FH (fermentation material original liquor) and positive control group Y (VE100 mg/kg), each group 12 rats. Except for the blank control group, the other groups were intraperitoneally injected with D-galactose (400 mg/kg.d), and the gastric dose was 0.2 ml/10 g 路d. Eight weeks after the experiment, the animals were killed, the organ index was calculated, and the histological observation and sex hormone were performed on the samples. The results showed that the uterine index and ovarian index in FM and Y groups were significantly higher than those in M group (P0.05). FM group, FH group and M group, P 0.05). FM group and FH group, respectively). The level of serum follicle stimulating hormone (FSH) in group Y was significantly lower than that in group M (P0.05). The levels of serum luteinizing hormone (LH) in each treatment group were significantly lower than those in M group (P0.05). Conclusion: the ferment of peanut meal natto has antioxidation effect, and the high dose of peanut meal ferment can delay the senescence of reproductive system in female rats. Experiment 2: effects of peanut meal ferment on diarrhea in mice; 40 Kunming mice were divided into blank control group A (saline), model group B (ampicillin) according to the weight of mice randomly divided into two groups: control group A (normal saline), model group B (ampicillin), Natural recovery group C (ampicillin saline) and HFY group D (ampicillin peanut meal fermentation broth). With the exception of the blank control group (A), all the treatment groups were administered with ampicillin every day (2 mg/g bw 路d, equivalent to 5 times the adult dose) until diarrhea occurred in each group and the model was completed. The (B) mice in the model group were killed and the intestinal contents were collected. (A) in blank control group, (C) in natural recovery group, (D) in HFY group were fed intragastrically with 0. 2 ml/10 路d, the mice were killed for 5 days, and the contents of intestinal tract were collected. Based on Illumina Hi Seq2500 sequencing platform, high-throughput sequencing of intestinal contents was carried out to explore the taxonomy and abundance of flora in intestinal contents. The results showed that at the gate level, the first three species in each experimental group were Bacteroides, thick-walled bacteria and Proteus. The relative content of Bacteroides in Group B (79.87%), C group, 65.90%) was in order, and the relative content of Bacteroides in Group B (79.87%) (65.90%) was the highest. Group A (58.22%) and Group D (45.10%). The order from high to low in each group of thick-walled fungi was D, A, C, B. The relative content of Proteus was in the order of group B (17.73%), C) (17.60%), A, 11.63%) and group D (6.92%) from high to low. NK4A136 and Rossiella in Group D were higher than those in other groups, Clostridium group D was higher than Group B, Group A and Group D were higher than Group B, and Group D was higher than Group B. Lactobacillus D group was higher than A group, B group, Enterococcus group D group was higher than A group; The differences of diversity index showed that there were differences in species abundance between group B and group B (P0.05). The species abundance of), D group was higher than that of the other three groups (P0.05). The results showed that peanut meal ferment could regulate the intestinal flora of diarrhea mice induced by antibiotics, increase the number of intestinal beneficial bacteria, inhibit the growth of harmful bacteria, and increase the abundance of intestinal flora and species evenness.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R151
本文編號：2442039
[Abstract]:In this study, peanut meal was used as the main raw material for solid fermentation of peanut meal by Bacillus natto. The effects of fermentation extract of natto peanut meal on delaying senescence of reproductive system in female rats and improving antibiotic-induced diarrhea in mice were investigated. Experiment one: the effect of peanut meal ferment on delaying the senescence of reproductive system in female rats; 72 female SD rats were randomly divided into blank control group N (normal saline) according to body weight and 72 female rats were randomly divided into two groups: control group N (NS), control group N (NS), Model group M (saline), low dose group FL (fermentation material 25 times diluent), medium dose group FM (fermentation material 5 times dilution liquid), high dose group FH (fermentation material original liquor) and positive control group Y (VE100 mg/kg), each group 12 rats. Except for the blank control group, the other groups were intraperitoneally injected with D-galactose (400 mg/kg.d), and the gastric dose was 0.2 ml/10 g 路d. Eight weeks after the experiment, the animals were killed, the organ index was calculated, and the histological observation and sex hormone were performed on the samples. The results showed that the uterine index and ovarian index in FM and Y groups were significantly higher than those in M group (P0.05). FM group, FH group and M group, P 0.05). FM group and FH group, respectively). The level of serum follicle stimulating hormone (FSH) in group Y was significantly lower than that in group M (P0.05). The levels of serum luteinizing hormone (LH) in each treatment group were significantly lower than those in M group (P0.05). Conclusion: the ferment of peanut meal natto has antioxidation effect, and the high dose of peanut meal ferment can delay the senescence of reproductive system in female rats. Experiment 2: effects of peanut meal ferment on diarrhea in mice; 40 Kunming mice were divided into blank control group A (saline), model group B (ampicillin) according to the weight of mice randomly divided into two groups: control group A (normal saline), model group B (ampicillin), Natural recovery group C (ampicillin saline) and HFY group D (ampicillin peanut meal fermentation broth). With the exception of the blank control group (A), all the treatment groups were administered with ampicillin every day (2 mg/g bw 路d, equivalent to 5 times the adult dose) until diarrhea occurred in each group and the model was completed. The (B) mice in the model group were killed and the intestinal contents were collected. (A) in blank control group, (C) in natural recovery group, (D) in HFY group were fed intragastrically with 0. 2 ml/10 路d, the mice were killed for 5 days, and the contents of intestinal tract were collected. Based on Illumina Hi Seq2500 sequencing platform, high-throughput sequencing of intestinal contents was carried out to explore the taxonomy and abundance of flora in intestinal contents. The results showed that at the gate level, the first three species in each experimental group were Bacteroides, thick-walled bacteria and Proteus. The relative content of Bacteroides in Group B (79.87%), C group, 65.90%) was in order, and the relative content of Bacteroides in Group B (79.87%) (65.90%) was the highest. Group A (58.22%) and Group D (45.10%). The order from high to low in each group of thick-walled fungi was D, A, C, B. The relative content of Proteus was in the order of group B (17.73%), C) (17.60%), A, 11.63%) and group D (6.92%) from high to low. NK4A136 and Rossiella in Group D were higher than those in other groups, Clostridium group D was higher than Group B, Group A and Group D were higher than Group B, and Group D was higher than Group B. Lactobacillus D group was higher than A group, B group, Enterococcus group D group was higher than A group; The differences of diversity index showed that there were differences in species abundance between group B and group B (P0.05). The species abundance of), D group was higher than that of the other three groups (P0.05). The results showed that peanut meal ferment could regulate the intestinal flora of diarrhea mice induced by antibiotics, increase the number of intestinal beneficial bacteria, inhibit the growth of harmful bacteria, and increase the abundance of intestinal flora and species evenness.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R151
【參考文獻】
相關期刊論文 前9條
1 劉瑞芳;趙華;李建華;程青虹;;益生菌酸奶對機體血脂水平影響的Meta分析[J];中國全科醫(yī)學;2016年32期
2 周曉丹;劉愛萍;張明;郭慧媛;任發(fā)政;;副干酪乳桿菌LC-01對便秘小鼠的通便作用[J];乳業(yè)科學與技術;2012年05期
3 張常娥;魏偉;劉英華;謝靜;;D-半乳糖亞急性衰老大鼠模型的建立及評價[J];中國老年學雜志;2012年04期
4 蔣萬志;張洪泉;;枸杞多肽對D-半乳糖誘導小鼠的抗衰老作用及其可能機制[J];國際藥學研究雜志;2010年01期
5 郭世奎;包維民;龔昆梅;胡大春;邵劍春;陳弟;王昆華;;實時熒光定量PCR法研究結(jié)直腸癌患者腸道擬桿菌屬、梭桿菌屬和梭菌屬量的變化[J];中國微生態(tài)學雜志;2010年01期
6 杜瑞亭;托婭;張和平;;干酪乳桿菌Zhang對小鼠腸道免疫功能的影響[J];營養(yǎng)學報;2009年04期
7 徐營,李霞,楊利國;雙歧桿菌的生物學特性及對人體的生理功能[J];微生物學通報;2001年06期
8 李文彬，，韋豐，范明，張京立，張炳烈，馬向晨，楊衛(wèi)平，魏文;D－半乳糖在小鼠上誘導的擬腦老化效應[J];中國藥理學與毒理學雜志;1995年02期
9 龔國清,徐黻本;小鼠衰老模型研究[J];中國藥科大學學報;1991年02期
相關博士學位論文 前1條
1 余欣慧;益腎續(xù)經(jīng)湯延緩絕經(jīng)過渡期大鼠卵巢功能衰老的實驗研究[D];湖北中醫(yī)藥大學;2010年
相關碩士學位論文 前1條
1 張文娣;抗生素相關性腹瀉模型的構(gòu)建[D];南方醫(yī)科大學;2015年
本文編號：2442039
本文鏈接：http://sikaile.net/yixuelunwen/yufangyixuelunwen/2442039.html
最近更新
教材專著
