【摘要】：目的研究鋁對大鼠認知能力及全基因組甲基化的影響。方法健康清潔級SD雄性大鼠72只,按體質量隨機分成兩部分9組,實驗部分:對照組(生理鹽水),低、中、高鋁劑量組(0.27、0.54和1.08 mg/kg);干預部分:對照組(生理鹽水),鋁高劑量組(1.08 mg/kg),鋁高劑量+葉酸低、中、高劑量組(0.7、1.5和3.4 mg/kg),每組8只。各組大鼠腹腔注射相應劑量麥芽酚鋁(0.2 ml/d),同時葉酸各劑量干預組每日灌胃葉酸1 ml/100 g,連續(xù)60 d。采用Morris水迷宮測定大鼠學習記憶能力,采用ELISA法測定大鼠全基因組甲基化。結果 Morris水迷宮結果顯示,鋁暴露可導致大鼠目標象限停留時間明顯縮短,穿越原平臺位置的次數明顯減少,鋁低、中、高劑量組與對照組比較差異有統(tǒng)計學意義(P0.05);葉酸干預鋁高劑量組可導致大鼠目標象限停留時間明顯延長和穿越原平臺位置的次數明顯增多,鋁高劑量組與鋁高+葉酸中、高劑量組比較差異有統(tǒng)計學意義(P0.05)。大鼠全基因組甲基化測定結果顯示:隨鋁染毒劑量的增加,0.54、1.08 mg/kg染毒組大鼠全基因組甲基化率顯著降低,與對照組比較差異具有統(tǒng)計學意義(P0.05);而添加高劑量葉酸組全基因組甲基化率顯著增加,與鋁高劑量組比較差異具有統(tǒng)計學意義(P0.05),與對照組比較差異無統(tǒng)計學意義。結論鋁可導致大鼠學習記憶能力下降,大鼠全基因組甲基化率降低,葉酸可能對其有改善作用。
[Abstract]:Objective to study the effects of aluminum on cognitive ability and whole genome methylation in rats. Methods 72 healthy and clean grade male SD rats were randomly divided into 9 groups according to body mass. The experimental part was as follows: control group (normal saline), low, middle, high aluminum dose group (0.27,0.54 and 1.08 mg/kg); Intervention part: control group (saline), high-dose aluminum group (1.08 mg/kg), high-dose aluminum folic acid low, middle, high-dose group (0.7, 1.5 and 3.4 mg/kg), 8 rats in each group. Rats in each group were intraperitoneally injected with the corresponding dose of maltol aluminum (0. 2 ml/d), and folic acid in the intervention group was given 1 ml/100 / g daily for 60 days. The learning and memory ability of rats was measured by Morris water maze, and the whole genome methylation of rats was determined by ELISA method. Results the results of Morris water maze showed that aluminum exposure could significantly shorten the retention time of the target quadrant, decrease the number of traversing the original platform position, and lower aluminum. There was significant difference between the middle and high dose groups compared with the control group (P0.05). The intervention of folic acid in the high-dose aluminum group could significantly prolong the target quadrant residence time and increase the times of crossing the original platform position. There was a significant difference between the high-dose aluminum group and the high-dose aluminum-folic acid group (P0.05). The results of whole genome methylation assay showed that the methylation rate of the whole genome in 0.54 and 1.08 mg/kg groups was significantly lower than that in the control group (P0.05). The whole genome methylation rate of the high-dose folic acid group was significantly higher than that of the high-dose aluminum group (P 0.05), but no significant difference was found between the high-dose folic acid group and the control group. Conclusion Aluminum can decrease the ability of learning and memory in rats and the methylation rate of whole genome in rats. Folic acid may improve the ability of learning and memory in rats.
【作者單位】： 山西醫(yī)科大學公共衛(wèi)生學院;山西醫(yī)科大學第一臨床醫(yī)學院老年病科;
【基金】：國家自然科學基金(No.81372968)
【分類號】：R114

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