被動吸煙對雄性大鼠及子代學習記憶影響的研究
發(fā)布時間：2019-02-14 20:29
【摘要】：目的:模擬人群在吸煙環(huán)境中的暴露狀態(tài),構建不同劑量及不同時間吸煙的雄性大鼠被動吸煙模型,評估吸煙對雄性大鼠及子代學習記憶的影響。方法:選用SD大鼠150只(雄性100只,雌性50只),隨機分為染毒組和空白對照組。雄鼠于處死前1周與雌鼠按1∶1合籠交配,雌鼠分娩后21天內觀察仔鼠發(fā)育情況。測定雄性大鼠及子代腦組織中SOD活力、MDA及NO含量;HE染色觀察腦、海馬組織形態(tài)學改變;IHC法檢測腦組織c-Fos及海馬組織BDNF表達情況;RT-PCR及Western Blot法檢測雄性大鼠及子代腦組織tau、海馬組織NR2B基因mRNA及蛋白表達。結果:1.染毒4周組的中劑量和高劑量雄性大鼠體重隨染毒劑量的增加而降低(P0.05)。2.各染毒組的雄性大鼠腦組織系數(shù)較對照組降低,差異有統(tǒng)計學意義(P0.05);各染毒組的仔鼠腦組織系數(shù)除4周組外,較對照組降低,差異有統(tǒng)計學意義(P0.05)。3.各染毒組的雄性大鼠腦SOD活力較對照組降低,差異有統(tǒng)計學意義(P0.05);各染毒組的仔鼠腦SOD活力較對照組降低,差異有統(tǒng)計學意義(P0.05)。4.各染毒組的雄性大鼠腦MDA含量較對照組上升,差異有統(tǒng)計學意義(P0.05);各染毒組的仔鼠腦MDA含量較對照組上升,差異有統(tǒng)計學意義(P0.05)。5.各染毒組的雄性大鼠腦NO含量除4周組外,較對照組上升,差異有統(tǒng)計學意義(P0.05);6周組的仔鼠腦NO含量,較對照組上升,差異有統(tǒng)計學意義(P0.05)。6.定位航行實驗中,雄性大鼠潛伏期結果與對照組相比,差異有統(tǒng)計學意義(P0.05),游泳距離結果與對照組相比,差異有統(tǒng)計學意義(P0.05);仔鼠潛伏期結果與對照組相比,差異有統(tǒng)計學意義(P0.05),游泳距離結果與對照組相比,差異有統(tǒng)計學意義(P0.05)。7.空間探索實驗中,雄性大鼠低、中、高劑量組中,不同染毒時間組比較,差異均有統(tǒng)計學意義(P0.05);仔鼠染毒4周組較對照組降低,差異有統(tǒng)計學意義(P0.05)。8.腦組織及海馬組織HE染色觀察發(fā)現(xiàn),染毒組雄性大鼠腦組織及海馬組織出現(xiàn)不同程度的損傷,并且隨著染毒時間延長損傷更加明顯;9.4周染毒組的雄性大鼠腦組織fos蛋白含量較對照組有所上升,差異有統(tǒng)計學意義(P0.05);各染毒組的仔鼠腦組織fos蛋白含量較對照組有所上升,差異有統(tǒng)計學意義(P0.05)。6、8周染毒組的雄性大鼠海馬組織BDNF蛋白含量較對照組有所上升,差異有統(tǒng)計學意義(P0.05);4、6周染毒組的仔鼠海馬組織BDNF蛋白含量較對照組有所上升,差異有統(tǒng)計學意義(P0.05)。10.與對照組相比,雄性大鼠6周中、高劑量組腦tau m RNA和蛋白表達水平增加明顯(P0.05);8周中、高劑量染毒組腦tau mRNA和蛋白表達較對照組相比出現(xiàn)明顯增加差異有統(tǒng)計學意義(P0.05);仔鼠染毒4周組高劑量,6周中、高劑量組,8周低劑量組腦組織tau蛋白表達水平較對照組升高,差異有統(tǒng)計學意義(P0.05)。與對照組相比,雄性大鼠6周染毒組組海馬NR2B mRNA和蛋白表達水平降低明顯(P0.05);8周染毒組海馬NR2B mRNA和蛋白表達較對照組相比出現(xiàn)明顯降低,差異有統(tǒng)計學意義(P0.05);仔鼠6、8周染毒組海馬NR2B mRNA和蛋白表達較對照組相比出現(xiàn)明顯降低,差異有統(tǒng)計學意義(P0.05)。結論:吸煙可導致雄性大鼠及子代行為學產(chǎn)生損傷改變,香煙暴露也可造成雄性大鼠及子代腦、海馬組織的損傷,導致腦及海馬組織蛋白及mRNA表達異常,提示吸煙可導致雄性大鼠及子代學習記憶的損傷。
[Abstract]:Objective: To study the effect of smoking on the learning and memory of male rats and children by simulating the exposure status of the population in the smoking environment, constructing the passive smoking model of the male rats with different doses and different time. Methods: 150 SD rats (100 males and 50 females) were randomly divided into exposure group and blank control group. Male rats were mated with female rats at 1: 1 in the first week before the sacrifice, and the development of the mice was observed in 21 days after the female. The activity of SOD, MDA and NO in the brain of the male and the offspring of the rats were measured. The morphological changes of the brain and the hippocampus were observed with HE staining. The expression of BDNF in the c-Fos and the hippocampus of the brain was detected by the IHC method, and the tau in the brain of the male and the offspring was detected by RT-PCR and Western Blot. mRNA and protein expression of NR2B gene in hippocampus. Results: 1. The weight of middle and high-dose male rats in the 4-week group decreased with the increase of the dose (P0.05). The brain tissue factor of the male rats in each group was lower than that in the control group (P <0.05). The brain tissue factor of the rats in each group was lower than that of the control group (P0.05). The activity of SOD in the brain of male rats in each group was lower than that in the control group (P <0.05). The activity of SOD in the brain of each group was lower than that in the control group (P <0.05). The content of MDA in the male rats of each group was higher than that in the control group (P <0.05). The content of MDA in the rats of each group was higher than that in the control group (P0.05). The NO content in the male rats of each group was higher than that in the control group except for the 4-week group (P0.05). The content of NO in the brain of the rats in the 6-week group was higher than that in the control group (P0.05). In the experiment of positioning, the latency of the male rats was significantly different from that of the control group (P0.05). The results of the swimming distance were statistically significant (P0.05). The results of swimming distance were statistically significant (P0.05). In the experiment of space exploration, in the low, middle and high dose group of male rats, the difference was significant (P0.05). The results showed that the level of fos protein in the brain and the hippocampus of the exposed group was higher than that in the control group. There was a significant difference in the content of fos protein in the hippocampus of each group (P0.05). The content of the expression of BDNF in the hippocampus of the rats in the 6 and 8-week group was higher than that in the control group (P <0.05). The content of BDNF protein in the hippocampus of the rats in 4 and 6 weeks was higher than that in the control group (P0.05). Compared with the control group, the level of tau m RNA and protein expression in the high-dose group was significantly higher than that in the control group (P0.05); in the 8-week period, the expression of tau mRNA and protein in the high-dose group was significantly higher than that of the control group (P0.05). The level of tau protein expression in the high-dose group and the high-dose group and the low-dose group in the 8-week low-dose group was significantly higher than that in the control group (P0.05). Compared with the control group, the expression of NR2B mRNA and protein in the hippocampus of the rats in the 6-week group was significantly lower than that in the control group (P <0.05). The expression of NR2B mRNA and protein in the hippocampus of the group was significantly lower than that of the control group (P0.05). The expression of NR2B mRNA and protein in the hippocampus of the rats in 6 and 8 weeks was significantly lower than that in the control group (P0.05). Conclusion: Smoking can cause damage to the behavior of male rats and offspring, and the exposure of cigarette can also cause the injury of the brain and hippocampus of the male and the offspring, which leads to the abnormal expression of the protein and the mRNA in the brain and the hippocampus. It is suggested that the smoking can lead to the learning and memory of the male and the offspring.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R114
[Abstract]:Objective: To study the effect of smoking on the learning and memory of male rats and children by simulating the exposure status of the population in the smoking environment, constructing the passive smoking model of the male rats with different doses and different time. Methods: 150 SD rats (100 males and 50 females) were randomly divided into exposure group and blank control group. Male rats were mated with female rats at 1: 1 in the first week before the sacrifice, and the development of the mice was observed in 21 days after the female. The activity of SOD, MDA and NO in the brain of the male and the offspring of the rats were measured. The morphological changes of the brain and the hippocampus were observed with HE staining. The expression of BDNF in the c-Fos and the hippocampus of the brain was detected by the IHC method, and the tau in the brain of the male and the offspring was detected by RT-PCR and Western Blot. mRNA and protein expression of NR2B gene in hippocampus. Results: 1. The weight of middle and high-dose male rats in the 4-week group decreased with the increase of the dose (P0.05). The brain tissue factor of the male rats in each group was lower than that in the control group (P <0.05). The brain tissue factor of the rats in each group was lower than that of the control group (P0.05). The activity of SOD in the brain of male rats in each group was lower than that in the control group (P <0.05). The activity of SOD in the brain of each group was lower than that in the control group (P <0.05). The content of MDA in the male rats of each group was higher than that in the control group (P <0.05). The content of MDA in the rats of each group was higher than that in the control group (P0.05). The NO content in the male rats of each group was higher than that in the control group except for the 4-week group (P0.05). The content of NO in the brain of the rats in the 6-week group was higher than that in the control group (P0.05). In the experiment of positioning, the latency of the male rats was significantly different from that of the control group (P0.05). The results of the swimming distance were statistically significant (P0.05). The results of swimming distance were statistically significant (P0.05). In the experiment of space exploration, in the low, middle and high dose group of male rats, the difference was significant (P0.05). The results showed that the level of fos protein in the brain and the hippocampus of the exposed group was higher than that in the control group. There was a significant difference in the content of fos protein in the hippocampus of each group (P0.05). The content of the expression of BDNF in the hippocampus of the rats in the 6 and 8-week group was higher than that in the control group (P <0.05). The content of BDNF protein in the hippocampus of the rats in 4 and 6 weeks was higher than that in the control group (P0.05). Compared with the control group, the level of tau m RNA and protein expression in the high-dose group was significantly higher than that in the control group (P0.05); in the 8-week period, the expression of tau mRNA and protein in the high-dose group was significantly higher than that of the control group (P0.05). The level of tau protein expression in the high-dose group and the high-dose group and the low-dose group in the 8-week low-dose group was significantly higher than that in the control group (P0.05). Compared with the control group, the expression of NR2B mRNA and protein in the hippocampus of the rats in the 6-week group was significantly lower than that in the control group (P <0.05). The expression of NR2B mRNA and protein in the hippocampus of the group was significantly lower than that of the control group (P0.05). The expression of NR2B mRNA and protein in the hippocampus of the rats in 6 and 8 weeks was significantly lower than that in the control group (P0.05). Conclusion: Smoking can cause damage to the behavior of male rats and offspring, and the exposure of cigarette can also cause the injury of the brain and hippocampus of the male and the offspring, which leads to the abnormal expression of the protein and the mRNA in the brain and the hippocampus. It is suggested that the smoking can lead to the learning and memory of the male and the offspring.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R114
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