CASP3和CASP7基因多態(tài)性與噪聲性聽力損失易感性關(guān)系
發(fā)布時間：2018-12-27 20:25
【摘要】：目的探討細胞凋亡通路相關(guān)基因CASP3和CASP7多態(tài)性與中國漢族人群噪聲性聽力損失(NIHL)易感性之間的關(guān)聯(lián)。方法研究對象來自2014年杭州市1 549名噪聲接觸工人聽力損失的橫斷面調(diào)查,采用1:1配對病例對照研究,病例組為電測聽雙耳高頻平均聽閾25 d B(A)的工人,對照組為性別、年齡、接噪工齡、工作崗位與病例匹配且雙耳所有頻段聽閾均≤25 d B(A)的工人,共272對。PCR-LDR法檢測2個SNP位點的基因型。采用多因素條件logistic回歸模型分析SNP位點與NIHL的關(guān)聯(lián),并以叉生分析計算基因-環(huán)境交互作用。結(jié)果χ~2檢驗分析發(fā)現(xiàn),CASP3基因rs1049216等位基因(C、T)頻率組間分布有統(tǒng)計學差異(OR=0.68,95%CI=0.50~0.93),而基因型頻率組間差異無統(tǒng)計學意義(P0.05);CASP7基因rs10787498等位基因及基因型頻率組間分布均無統(tǒng)計學意義(P0.05);多因素條件logistic回歸顯示,CASP3基因rs1049216中,與野生基因型CC相比,突變基因型(CT+TT)為NIHL的保護因素(調(diào)整OR=0.65,95%CI=0.43~0.97);叉生分析表明rs1049216位點與文化程度、睡眠時間存在交互作用(P≤0.001),NIHL的危險性降低(OR值變小)。結(jié)論 CASP3基因rs1049216位點可能與中國漢族人群NIHL易感性有關(guān),且可能與文化程度、睡眠時間存在交互作用。
[Abstract]:Objective to investigate the association between the polymorphisms of CASP3 and CASP7 genes associated with apoptosis pathway and the susceptibility to noise-induced hearing loss (NIHL) in Chinese Han population. Methods A cross-sectional investigation of hearing loss among 1 549 noise exposed workers in Hangzhou in 2014 was carried out. A 1:1 matched case-control study was conducted. In the control group, there were 272 pairs of workers who had sex, age, length of exposure to noise, job matching with case and hearing threshold of less than 25 d B (A) at all frequencies. The genotypes of two SNP loci were detected by PCR-LDR method. Multivariate conditional logistic regression model was used to analyze the relationship between SNP locus and NIHL, and the interaction between gene and environment was calculated by cross analysis. Results 蠂 ~ 2 test showed that the distribution of rs1049216 allele (CITT) frequency of CASP3 gene was significantly different (OR=0.68,95%CI=0.50~0.93), but there was no significant difference between genotype frequency groups (P0.05). The distribution of rs10787498 allele and genotype frequency of CASP7 gene were not statistically significant (P0.05). Multivariate conditional logistic regression showed that the mutant (CT TT) was the protective factor of NIHL (adjusted OR=0.65,95%CI=0.43~0.97) in the rs1049216 of CASP3 gene compared with the wild genotype CC. The permutation analysis showed that there was interaction between rs1049216 locus and education level and sleep time (P 鈮，
本文編號：2393559
[Abstract]:Objective to investigate the association between the polymorphisms of CASP3 and CASP7 genes associated with apoptosis pathway and the susceptibility to noise-induced hearing loss (NIHL) in Chinese Han population. Methods A cross-sectional investigation of hearing loss among 1 549 noise exposed workers in Hangzhou in 2014 was carried out. A 1:1 matched case-control study was conducted. In the control group, there were 272 pairs of workers who had sex, age, length of exposure to noise, job matching with case and hearing threshold of less than 25 d B (A) at all frequencies. The genotypes of two SNP loci were detected by PCR-LDR method. Multivariate conditional logistic regression model was used to analyze the relationship between SNP locus and NIHL, and the interaction between gene and environment was calculated by cross analysis. Results 蠂 ~ 2 test showed that the distribution of rs1049216 allele (CITT) frequency of CASP3 gene was significantly different (OR=0.68,95%CI=0.50~0.93), but there was no significant difference between genotype frequency groups (P0.05). The distribution of rs10787498 allele and genotype frequency of CASP7 gene were not statistically significant (P0.05). Multivariate conditional logistic regression showed that the mutant (CT TT) was the protective factor of NIHL (adjusted OR=0.65,95%CI=0.43~0.97) in the rs1049216 of CASP3 gene compared with the wild genotype CC. The permutation analysis showed that there was interaction between rs1049216 locus and education level and sleep time (P 鈮，
本文編號：2393559
本文鏈接：http://sikaile.net/yixuelunwen/yufangyixuelunwen/2393559.html
最近更新
教材專著
