【摘要】：[目的]探索某魚(yú)油產(chǎn)品對(duì)細(xì)顆粒物(PM2.5)氣管滴注染毒引起的肺部急性毒性的干預(yù)作用。[方法]將36只雄性SD大鼠隨機(jī)分為6組,即溶劑(玉米油)對(duì)照組、魚(yú)油(0.60 g/kg)對(duì)照組、PM2.5染毒(8.0 mg/kg)對(duì)照組、PM2.5+低、中、高劑量(0.15、0.30、0.60 g/kg)魚(yú)油給藥組。PM2.5染毒對(duì)照組、魚(yú)油給藥組分別經(jīng)正常飼養(yǎng)、魚(yú)油灌胃28 d后,行氣管滴注染毒PM2.5,滴注量為8.0 mg/kg,隔天1次,共3次。魚(yú)油對(duì)照組行魚(yú)油灌胃35 d,溶劑對(duì)照組先后行玉米油灌胃(28 d)和生理鹽水氣管滴注(7 d)。末次染毒后24 h,行肺灌洗,收集支氣管肺泡灌洗液,之后切除肺部,制作肺病理切片,分析測(cè)定肺灌洗液中白細(xì)胞介素1β(IL-1β)、白細(xì)胞介素6(IL-6)、腫瘤壞死因子α(TNF-α)、Clara細(xì)胞蛋白(CC16蛋白)、超敏C反應(yīng)蛋白(hs-CRP)、丙二醛(MDA)、總超氧化物歧化酶(T-SOD)的含量。[結(jié)果]與溶劑對(duì)照組相比:PM2.5染毒對(duì)照組大鼠炎癥指標(biāo)IL-1β、IL-6、hs-CRP和TNF-α升高,CC16蛋白降低;氧化應(yīng)激指標(biāo)T-SOD降低(均P0.05)。與PM2.5染毒對(duì)照組相比:各劑量的魚(yú)油給藥組大鼠IL-1β、IL-6、hs-CRP和TNF-α的釋放量均降低,CC16蛋白含量升高,T-SOD含量升高(均P0.05)。各實(shí)驗(yàn)組間MDA含量差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。[結(jié)論]PM2.5氣管滴注染毒可以引起大鼠肺部急性損傷,導(dǎo)致炎性因子和氧化應(yīng)激的變化,該種魚(yú)油產(chǎn)品喂飼對(duì)PM2.5急性染毒引起的大鼠肺部損傷具有一定的保護(hù)作用。
[Abstract]:Objective] to explore the effect of fish oil on pulmonary acute toxicity induced by tracheal drip of fine particulate matter (PM2.5). [methods] Thirty-six male SD rats were randomly divided into 6 groups: solvent (corn oil) control group, fish oil (0.60 g/kg) control group, PM2.5 (8.0 mg/kg) control group, low and moderate PM2.5. High dose (0. 15? 0. 30? 0. 60 g/kg) of fish oil group. The control group and the fish oil administration group were fed with normal diet. After 28 days of intragastric administration of fish oil, the volume of PM2.5, was 8.0 mg/kg, by trachea instillation of PM2.5,. Once every other day, 3 times. Fish oil control group was given fish oil for 35 days, solvent control group was given corn oil (28 days) and normal saline trachea drip (7 d). Lung lavage was performed 24 hours after the last exposure, and bronchoalveolar lavage fluid was collected. After lung resection, lung pathological sections were made, and interleukin 1 尾 (IL-1 尾) and interleukin 6 (IL-6) in lung lavage fluid were analyzed and measured. The contents of TNF- 偽), Clara cell protein (CC16 protein), hypersensitive C-reactive protein (hs-CRP) and malondialdehyde (MDA), total superoxide dismutase (T-SOD). [results] compared with the solvent control group, the inflammatory indexes IL-1 尾, IL-6,hs-CRP and TNF- 偽 were increased and CC16 protein was decreased, and the oxidative stress index T-SOD was decreased in PM2.5 exposed control group (P0.05). Compared with the control group exposed to PM2.5, the release of IL-1 尾, IL-6,hs-CRP and TNF- 偽 decreased, the content of CC16 protein increased and the content of T-SOD increased in each dose of fish oil administration group (P0.05). There was no significant difference in MDA content among the experimental groups (P0.05). [conclusion] tracheal instillation of PM2.5 can cause acute lung injury, inflammatory factors and oxidative stress in rats. This fish oil product can protect lung injury induced by acute PM2.5 exposure in rats.
【作者單位】： 復(fù)旦大學(xué)公共衛(wèi)生學(xué)院公共衛(wèi)生安全教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(編號(hào):81172617)
【分類號(hào)】：R151

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10 朱可s，

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