【摘要】：目的研究楊梅酮(myricetin,MTN)對高脂膳食小鼠學(xué)習(xí)記憶能力的影響。方法 64只C57BL/6J雄性小鼠隨機(jī)分為對照組(CN,基礎(chǔ)飼料),楊梅酮對照組(MTN,基礎(chǔ)飼料+0.08%楊梅酮),高脂組(HF,高脂飼料),高脂+0.08%楊梅酮組(HF+MTN)4組。6w后采用Morris水迷宮測定小鼠空間學(xué)習(xí)記憶能力。以基因芯片分別探究高脂膳食與楊梅酮對海馬信號通路的影響,q PCR法驗(yàn)證基因芯片結(jié)果。檢測小鼠海馬的氧化應(yīng)激指標(biāo)、炎性反應(yīng)指標(biāo)和BDNF的水平和相對表達(dá)量。結(jié)果與CN組相比,HF組小鼠學(xué)習(xí)記憶能力和BDNF水平顯著下降(P0.05);海馬SOD和GSH-Px活性顯著下降,MDA水平顯著升高(P0.05),但TNF-α、IL-6和MCP-1水平無顯著變化(P0.05)。長時(shí)程增強(qiáng)信號通路和過氧化物酶體信號通路是高脂膳食和楊梅酮共同改變的信號通路(P0.05),高脂誘導(dǎo)海馬Sod2、Prdx1、Prdx5、Creb、Itpr1和Plcb3表達(dá)顯著下降。HF+MTN組認(rèn)知障礙顯著改善,海馬SOD活性顯著升高,MDA水平顯著降低,顯著上調(diào)Prdx1、Prdx5、Creb和Itpr1的表達(dá)(P0.05)。結(jié)論楊梅酮通過維持海馬氧化還原狀態(tài),調(diào)節(jié)長時(shí)程增強(qiáng)信號通路,可改善小鼠的學(xué)習(xí)記憶能力。
[Abstract]:Objective to study the effect of myricetin (myricetin,MTN) on learning and memory ability in high fat diet mice. Methods 64 male C57BL/6J mice were randomly divided into three groups: control group (CN, basic diet), myricetin control group (MTN, basic diet 0.08% myriceone) and high fat group (HF, high fat diet). After 6 weeks, the spatial learning and memory ability of mice was measured by Morris water maze. The effects of high fat diet and myricetin on hippocampal signal pathway were investigated by gene chip, q PCR method. The levels and relative expression of BDNF and oxidative stress index, inflammatory response index and BDNF in the hippocampus of mice were measured. Results compared with CN group, the ability of learning and memory and the level of BDNF in HF group decreased significantly (P0.05). The activities of SOD and GSH-Px in hippocampus decreased significantly, and the level of MDA increased significantly (P0.05), but the levels of TNF- 偽, IL-6 and MCP-1 did not change significantly (P0.05). Long-term potentiation signal pathway and peroxisome signal pathway were the signal pathways of hyperlipidemia diet and myricetin (P0.05), and hyperlipidemia induced hippocampal Sod2,Prdx1,Prdx5,Creb,. The expression of Itpr1 and Plcb3 decreased significantly in. HF MTN group. In. HF MTN group, the cognitive impairment, the activity of SOD and the level of MDA in hippocampus were significantly increased, and the expression of Prdx1,Prdx5,Creb and Itpr1 were significantly up-regulated (P0.05). Conclusion Myricetin can improve the learning and memory ability of mice by maintaining the redox state of hippocampus and regulating the long term enhancement of signal pathway.
【作者單位】： 江南大學(xué)無錫醫(yī)學(xué)院;江南大學(xué)食品科學(xué)與技術(shù)國家重點(diǎn)實(shí)驗(yàn)室;中糧集團(tuán)油脂部;
【基金】：中央高校基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金資助(No.JUSRP115A33)
【分類號】：R151

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