【摘要】：黑大豆不僅具有較高的營養(yǎng)價值，且含有多種植物化學物�；ㄉ帐呛诖蠖沟闹饕锘钚晕镔|(zhì)�；ㄉ站哂锌寡趸�、抗炎、改善視力、改善脂代謝、保護心血管等諸多保健功效。目前尚缺少黑大豆種皮提取物對酒精所致化學性肝損傷干預作用的報道。 目的： 以小鼠急性酒精性肝損傷為模型，通過對小鼠肝臟還原性谷胱甘肽、肝臟丙二醛、甘油三酯含量測定、肝臟組織病理切片等分析，探討黑大豆種皮提取物對酒精性肝損傷的影響。 材料與方法： 雄性昆明小鼠，隨機分為正常對照組、酒精模型組、黑大豆種皮提取物低劑量組（30mg·kg-1）、黑大豆種皮提取物高劑量組（60mg·kg-1）。按照《保健食品檢驗與評價技術(shù)規(guī)范（2003版）》的要求，每日灌胃一次，第31天，除正常對照組外，一次性灌胃給予50%無水乙醇溶液，造成急性酒精性肝損傷，禁食不禁水12h后檢測各組小鼠的肝臟系數(shù)、肝臟甘油三酯（TG）、肝臟丙二醛（MDA）、腦組織MDA、肝臟還原型谷胱甘肽（GSH）、全血GSH的含量，觀察肝臟的病理組織學改變并進行肝組織脂肪病變評分。應用SPSS18.0統(tǒng)計軟件，進行單因素方差分析或秩和檢驗。 結(jié)果： 1．黑大豆種皮提取物高劑量組動物的肝臟組織GSH含量，明顯高于酒精肝損傷模型組（P＜0.05），且與正常對照差異不明顯。黑大豆皮提取物低劑量組動物的肝臟組織GSH含量與肝損傷模型對照組比較，差異不明顯。 2.黑大豆皮提取物低劑量組和高劑量組動物的肝臟組織MDA含量均低于酒精肝損傷模型組（P＜0.05），且與正常對照差異不明顯。 3．黑大豆種皮提取物低劑量組和高劑量組動物的肝臟脂肪病變程度，均明顯低于酒精肝損傷模型組（P＜0.05）。 結(jié)論： 黑大豆種皮提取物可以降低急性酒精性肝損傷動物的肝臟丙二醛（MDA）含量，防止肝組織還原型谷胱甘肽（GSH）的耗竭，改善急性酒精性肝損傷引起的肝臟病理組織學變化，，減輕肝臟脂肪變性的程度，有助于降低酒精性肝損傷。
[Abstract]:Black soybean not only has high nutritional value, but also contains many plant chemicals. Anthocyanin is the main bioactive substance of black soybean. Anthocyanins have many health effects such as anti-oxidation, anti-inflammation, improving vision, improving lipid metabolism, protecting cardiovascular system and so on. At present, there is no report on the effect of black soybean seed coat extract on alcohol-induced chemical liver injury. Objective: to determine the contents of reductive glutathione, malondialdehyde and triglyceride in the liver of mice with acute alcoholic liver injury. To investigate the effect of black soybean seed coat extract on alcoholic liver injury. Materials and methods: male Kunming mice were randomly divided into normal control group, alcohol model group, black soybean seed coat extract low dose group (30mg kg-1), black soybean seed coat extract high dose group (60mg kg-1). According to the Technical Specification for Health Food Inspection and Evaluation (2003 Edition), once a day, 31 days, 50% anhydrous ethanol solution was given once a day in addition to the normal control group, resulting in acute alcoholic liver injury. After fasting for 12 hours, the liver coefficient of each group was determined, and the content of GSH in the whole blood of MDA, liver, glutathione (GSH), MDA, liver, malondialdehyde (MDA) (MDA), brain tissue and liver triglyceride (TG),) were measured. The histopathological changes of liver were observed and hepatic fat lesion score was evaluated. Single factor ANOVA or rank sum test were performed with SPSS18.0 software. Results: 1. The content of GSH in liver of the high dose group of black soybean seed coat extract was significantly higher than that of the model group of alcoholic liver injury (P < 0. 05), and there was no significant difference between the high dose group and the normal control group (P < 0. 05). The content of GSH in liver tissue of the low dose group of black soybean peel extract was not significantly different from that of the model control group. 2. The content of MDA in liver tissue of low dose group and high dose group of black soybean peel extract was lower than that of alcohol induced liver injury group (P < 0. 05), and there was no significant difference compared with the normal control group (P < 0. 05). 3. The degree of hepatic fatty lesion in low dose and high dose groups of black soybean seed coat extract was significantly lower than that of alcohol induced liver injury model group (P < 0.05). Conclusion: black soybean seed coat extract can reduce malondialdehyde (MDA) content in liver and prevent the depletion of glutathione (GSH) in liver tissue. Improving the histopathological changes of liver caused by acute alcoholic liver injury and reducing the degree of hepatic steatosis are helpful to reduce alcoholic liver injury.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R151

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