【摘要】：[目的]研究納米二氧化硅(nano-SiO_2)對(duì)人肺腺癌A549細(xì)胞的毒性作用。[方法]將A549細(xì)胞隨機(jī)分成6組,即對(duì)照組和實(shí)驗(yàn)組(5、10、25、50、100 mg/L nano-SiO_2組)。實(shí)驗(yàn)組分別用不同濃度的nano-SiO(210~20 nm粒徑)刺激,MTT法檢測(cè)細(xì)胞活性,比色法測(cè)定乳酸脫氫酶(LDH)活性,透射電子顯微鏡觀察細(xì)胞微觀結(jié)構(gòu),流式細(xì)胞儀檢測(cè)nano-SiO_2對(duì)細(xì)胞周期以及細(xì)胞凋亡的影響。[結(jié)果]與對(duì)照組相比:隨著nano-SiO_2濃度升高,A549細(xì)胞存活率呈降低趨勢(shì);LDH活性增加(P0.01或P0.05),在濃度為0、5、10、25 mg/L時(shí)呈濃度依賴關(guān)系,但在nano-SiO_2濃度為50 mg/L時(shí),LDH活力降低,100 mg/L時(shí)增高。nano-SiO_2濃度的升高可導(dǎo)致細(xì)胞膜表面絨毛凸起減少,細(xì)胞器空泡樣變?cè)黾?次級(jí)溶酶體增多;細(xì)胞凋亡率增加,壞死率增加;細(xì)胞在G0/G1期阻滯,增殖受抑制。[結(jié)論]nano-SiO_2作用于A549細(xì)胞可被細(xì)胞攝取,使細(xì)胞器結(jié)構(gòu)功能受損,從而抑制細(xì)胞增殖、誘導(dǎo)細(xì)胞凋亡,引起細(xì)胞周期紊亂。
[Abstract]:[objective] to study the toxic effect of nano silica (nano-SiO_2) on human lung adenocarcinoma cell line A549. [methods] A549 cells were randomly divided into 6 groups, namely control group and experimental group (50 mg/L nano-SiO_2 group). The experimental group was stimulated with different concentrations of nano-SiO (210 ~ 20 nm diameter), the cell activity was detected by MTT method, the activity of lactate dehydrogenase (LDH) was measured by colorimetry, and the microstructure of cells was observed by transmission electron microscope (TEM). The effect of nano-SiO_2 on cell cycle and apoptosis was detected by flow cytometry. [results] compared with the control group, the survival rate of A549 cells decreased with the increase of nano-SiO_2 concentration. The activity of LDH increased (P0.01 or P0.05) in a concentration-dependent manner when the concentration of nano-SiO_2 was 1025 mg/L, but when the concentration of nano-SiO_2 was 50 mg/L, the activity of LDH decreased. At 100 mg/L, the increase of nano-SiO_2 concentration resulted in the decrease of villi bulge, the vacuolation of organelle and the increase of secondary lysosome. Apoptosis rate increased, necrosis rate increased, cell proliferation was inhibited in G0/G1 phase. [conclusion] the effect of nano-SiO_2 on A549 cells can be taken up by the cells and damage the organelle structure and function, thus inhibit cell proliferation, induce apoptosis and cause cell cycle disorder.
【作者單位】： 華北理工大學(xué)基礎(chǔ)醫(yī)學(xué)院;華北理工大學(xué)醫(yī)學(xué)實(shí)驗(yàn)研究中心;華北理工大學(xué)國(guó)家科技部老年醫(yī)學(xué)國(guó)際科技合作基地;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(編號(hào):81673119) 華北理工大學(xué)大學(xué)生創(chuàng)新項(xiàng)目(編號(hào):X2016213) 唐山市老年醫(yī)學(xué)科技創(chuàng)新團(tuán)隊(duì)(編號(hào):15130212C)
【分類號(hào)】：R114

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