【摘要】：目的分析不同受照劑量和不同受照時(shí)間對(duì)人周圍血淋巴細(xì)胞生長(zhǎng)抑制和DNA損傷基因45(Gadd45)mRNA相對(duì)表達(dá)水平的影響,探討Gadd45基因作為生物劑量計(jì)的可能性。方法 1劑量-效應(yīng)關(guān)系研究。以0.00、0.10、0.25、0.50、1.00、2.00、3.00和5.00 Gy劑量X射線離體照射健康人周圍血樣,分離淋巴細(xì)胞進(jìn)行檢測(cè);2時(shí)間-效應(yīng)關(guān)系研究。以2.00 Gy劑量X射線離體照射健康人周圍血樣,分離淋巴細(xì)胞后分別于培養(yǎng)0、3、6、12、24和48 h時(shí)收獲細(xì)胞。采用實(shí)時(shí)熒光定量聚合酶鏈反應(yīng)法檢測(cè)Gadd45 mRNA相對(duì)表達(dá)水平。結(jié)果 1 0.00~0.50 Gy劑量范圍內(nèi),Gadd45 mRNA相對(duì)表達(dá)水平隨照射劑量的增加而增加,回歸方程為y=1.056 9+7.132 2 x[決定系數(shù)(R2)=0.992,P0.01]。0.50 Gy劑量組Gadd45 mRNA相對(duì)表達(dá)水平達(dá)到峰值,為0.00 Gy劑量組的4.53倍;0.50~5.00 Gy劑量范圍內(nèi),Gadd45 mRNA相對(duì)表達(dá)水平呈波動(dòng)性下降。22.00 Gy劑量照射后3~12 h,Gadd45 mRNA相對(duì)表達(dá)水平隨照射時(shí)間的增加而增加,回歸方程為y=-6.366 0+4.965 0 x(R2=0.932,P0.05)。照射后12 h時(shí)間組Gadd45 mRNA相對(duì)表達(dá)水平達(dá)到峰值,為0 h時(shí)間組的57.68倍;Gadd45 mRNA相對(duì)表達(dá)水平在照射后24~48 h大幅度下降,照射后48 h時(shí)間組為0 h時(shí)間組的2.08倍。結(jié)論 X射線照射可致Gadd45 mRNA表達(dá)上調(diào),在一定劑量和時(shí)間范圍內(nèi)具有良好的劑量-效應(yīng)和時(shí)間-效應(yīng)關(guān)系;但Gadd45基因作為新型生物劑量計(jì)仍需進(jìn)一步研究驗(yàn)證。
[Abstract]:Objective to analyze the effects of different doses and time of irradiation on the growth inhibition of human peripheral blood lymphocytes and the relative expression of DNA damage gene 45 (Gadd45) mRNA, and to explore the possibility of Gadd45 gene as a biological dosimeter. Methods 1 the dose-effect relationship was studied. The peripheral blood samples of healthy people were irradiated in vitro with the doses of 0.000.10 ~ 0.25 ~ 0.50 ~ 1.00 ~ 1.00 ~ 2.00 ~ 3.00 and 5.00 Gy respectively, and the relationship between time and effect was studied. Peripheral blood samples of healthy people were irradiated with 2. 00 Gy dose of X ray in vitro. Lymphocytes were isolated and cultured for 24 h and 48 h, respectively. The relative expression of Gadd45 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction. Results within the dose range of 0.50 Gy, the relative expression of Gadd45 mRNA increased with the increase of irradiation dose. The regression equation was 1.056 9 7.132 2 x [determination coefficient (R2) = 0.992 2]. P0.01. The relative expression of Gadd45 mRNA in 0.50 Gy group reached the peak value, 4.53 times of that in 0.00 Gy group. Within the dose range of 0.50 Gy 5.00 Gy, the relative expression level of Gadd45 mRNA decreased, and the relative expression level of Gadd45 mRNA increased with the increase of irradiation time at 3h 12 h after 22.00 Gy dose irradiation. The regression equation was Yi-6.366 0 4.965 x (R _ 2N _ (0.932) P _ (0.05). The relative expression level of Gadd45 mRNA reached the peak at 12 h after irradiation, which was 57.68 times of that in 0 h group, and the relative expression level of Gadd45 mRNA decreased significantly at 24 h after irradiation 48 h after irradiation, and 2.08 times as much as 0 h at 48 h after irradiation. Conclusion the expression of Gadd45 mRNA can be upregulated by X-ray irradiation, which has a good dose-effect and time-effect relationship within a certain dose and time range, but the Gadd45 gene as a new biological dosimeter needs further study and verification.
【作者單位】： 廣東省職業(yè)病防治院廣東省職業(yè)病防治重點(diǎn)實(shí)驗(yàn)室;
【基金】：廣東省職業(yè)病防治重點(diǎn)實(shí)驗(yàn)室(2012A061400007) 廣東省職業(yè)病防治院課題(Z201004)
【分類號(hào)】：R144

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 潘艷;李玉文;封江彬;陸雪;劉青杰;蘇旭;;~(60)Coγ射線照射淋巴細(xì)胞誘導(dǎo)PIG3和GADD45基因mRNA變化[J];輻射研究與輻射工藝學(xué)報(bào);2008年05期

2 劉建功;劉紅艷;黨旭紅;張忠新;張慧芳;段志凱;左雅慧;;~(12)C離子照射對(duì)MDM2和GADD45A基因表達(dá)的影響[J];輻射防護(hù)通訊;2014年02期

【共引文獻(xiàn)】

相關(guān)期刊論文 前3條

1 李建國(guó);聞建華;左雅慧;秦秀軍;張偉;王小莉;;0.2 Gy γ射線照射人外周血淋巴細(xì)胞不同時(shí)間點(diǎn)差異基因表達(dá)譜研究[J];輻射防護(hù);2010年04期

2 張德欽;劉青杰;;p53通路相關(guān)基因表達(dá)作為輻射生物劑量計(jì)的研究現(xiàn)狀[J];輻射研究與輻射工藝學(xué)報(bào);2011年02期

3 黨旭紅;左雅慧;段志凱;郭萬(wàn)龍;劉建功;;~(60)Coγ射線誘發(fā)人外周血GADD45A基因表達(dá)的變化研究[J];中國(guó)輻射衛(wèi)生;2011年03期

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 黨旭紅;左雅慧;段志凱;郭萬(wàn)龍;劉建功;;~(60)Coγ射線誘發(fā)人外周血GADD45A基因表達(dá)的變化研究[J];中國(guó)輻射衛(wèi)生;2011年03期

2 段志凱;劉建功;郭萬(wàn)龍;張淑賢;;不同劑量γ射線誘發(fā)MDM2基因表達(dá)初步研究[J];中國(guó)職業(yè)醫(yī)學(xué);2011年01期

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 鄧志誠(chéng);;第七屆ICRR關(guān)于輻射遺傳效應(yīng)和生物劑量計(jì)的研究概況[J];輻射防護(hù)通訊;1983年06期

相關(guān)會(huì)議論文 前1條

1 江波;王曉光;姜恩海;;早熟染色體凝集(PCC)技術(shù)測(cè)量輻射誘導(dǎo)的間期染色體損傷及作為生物劑量計(jì)的研究[A];全國(guó)醫(yī)用輻射防護(hù)與安全學(xué)術(shù)研討會(huì)論文匯編[C];2004年

相關(guān)博士學(xué)位論文 前1條

1 張曉紅;基于表面等離激元共振技術(shù)的生物劑量計(jì)研究[D];南京航空航天大學(xué);2013年

相關(guān)碩士學(xué)位論文 前3條

1 蔣亞齊;基于輻射誘發(fā)染色體和DNA損傷的生物劑量計(jì)可行性研究[D];蘇州大學(xué);2007年

2 樂(lè)晨;血液成分與輻射劑量關(guān)系的初步研究[D];第二軍醫(yī)大學(xué);2007年

3 信亞周;CA誘導(dǎo)早熟凝集染色體作為生物劑量計(jì)的研究[D];濟(jì)南大學(xué);2013年

，

本文編號(hào)：2355904