【摘要】：本文詳細論述了以蜂蠟為原料制備二十八烷醇的工藝，并進行優(yōu)化；其次以SD大鼠為實驗模型,研究了二十八烷醇對于SD大鼠生長性能、血液生化指標、激素分泌量等的影響；最后通過分子生物學實驗，研究了二十八烷醇對于SD大鼠機體參與能量代謝過程基因的表達量的影響，深入研究了對于大鼠能量代謝的調控機制。 試驗一：實驗首先將精制蜂蠟用醇相皂化法處理，研究了皂化過程中所需要的皂化溫度、皂化時間、NaOH乙醇溶液濃度、固液比四個單一因素對于皂化提取過程的影響，確定了在單因素條件下的最佳條件，然后通過正交試驗設計確定最佳的皂化條件為皂化溫度95℃、皂化時間3.5h、NaOH乙醇溶液濃度為8%、固液比為1:8。然后經過分子蒸餾技術純化可以制得最高純度89.78％的二十八烷醇。 試驗二：以SD大鼠為模型，，在飼料中添加了不同濃度的二十八烷醇，研究了二十八烷醇對于SD大鼠生長性能、生理生化參數(shù)、激素分泌量的影響。實驗選用了48只健康的SD大鼠（雌雄各半），按照體重一致原則分為4組：實驗1組（生理鹽水空白對照組）、實驗2組（低二十八烷醇濃度組）、實驗3組（中二十八烷醇濃度組）、實驗4組（高二十八烷醇濃度組）。大鼠在飼養(yǎng)期間自由采食，飲用蒸餾水，試驗時間為30天。記錄采食量、體重等各項指標。實驗后期采集大鼠血液、肝臟、肌肉等組織樣品，熒光定量GLUT-4、StAR、GS、AMPK等多個基因的表達。試驗結果顯示： 1相對于生理鹽水空白對照組，飼喂高、中、低三個不同濃度的二十八烷醇乳化劑后顯著提高SD大鼠的體重、日增重和飼料轉化率，對于生理生化參數(shù)影響顯著；SD大鼠生長激素、胰高血糖素等分泌量均明顯增加。 2相對于生理鹽水空白對照組，飼喂高、中、低三個不同濃度的二十八烷醇乳化劑后SD大鼠體內總膽固醇、低密度脂蛋白顯著降低，高密度脂蛋白明顯增加；中濃度實驗組的調節(jié)作用最佳。 3相對于生理鹽水空白對照組，飼喂高、中、低不同濃度的二十八烷醇乳化劑后SD大鼠肝臟和肌肉組織中GLUT-4、STAR、GS、AMPK等多個基因均高于對照組，且中濃度實驗組表達量增加最明顯。
[Abstract]:In this paper, the preparation process of 28 alkanol from beeswax was discussed in detail and optimized. Secondly, the effects of 28 alkanol on growth performance, blood biochemical index and hormone secretion of SD rats were studied using SD rats as experimental model. Finally, the effects of 28 alkanol on the expression of genes involved in energy metabolism in SD rats were studied by molecular biological experiments, and the regulation mechanism of energy metabolism in rats was also studied. Experiment 1: the refined beeswax was first treated by alcohol phase saponification method. The effects of four single factors, such as saponification temperature, saponification time, concentration of NaOH ethanol solution and solid-liquid ratio, on the saponification extraction process were studied. The optimum conditions were determined under the single factor condition, and the optimum saponification conditions were determined by orthogonal design: saponification temperature 95 鈩

本文編號：2337213