【摘要】：目的:研究納米銀對人肺癌(A549)和人肝癌(HepG2)細(xì)胞系增殖和凋亡的影響,并探討納米銀對兩種細(xì)胞系毒效應(yīng)的差異及其機(jī)制。方法:用20、40、80、160、320、640μg/mL的納米銀分別染毒A549和HepG2細(xì)胞,染毒時長均為24和48 h,以細(xì)胞培養(yǎng)液為對照,采用MTT法檢測各組細(xì)胞的存活率;谷胱甘肽(GSH)和超氧化物歧化酶(SOD)試劑盒檢測細(xì)胞內(nèi)GSH含量和SOD活力。除上述各染毒組,兩種細(xì)胞均另設(shè)置N-乙酰半胱氨酸(NAC)預(yù)處理后160μg/mL納米銀染毒組,采用流式細(xì)胞術(shù)檢測各組細(xì)胞凋亡率。結(jié)果:與對照組比較,染毒24和48 h后,≥40μg/mL劑量組A549細(xì)胞和所有劑量組HepG2細(xì)胞存活率均降低(P0.05或P0.01);與相同染毒條件下的A549細(xì)胞比較,40~640μg/mL劑量組HepG2細(xì)胞的存活率較高(P0.05或(P0.01)。SOD活力和GSH含量檢測發(fā)現(xiàn),納米銀染毒A549細(xì)胞24 h后,40 pg/mL劑量組SOD活力與對照組比較顯著降低(P0.05),而GSH含量上升(P0.05);染毒48 h后,SOD活力和GSH含量在40~160μg/mL劑量組下降,其中80μg/mL劑量組GSH含量與對照組間的差異顯著(P0.05)。納米銀染毒HepG2細(xì)胞24、48 h后,SOD活力和GSH含量都表現(xiàn)為上升,其中40、80μg/mL組SOD活力和20μg/mL組GSH含量與對照組間的差異顯著(P0.05)。細(xì)胞凋亡率檢測發(fā)現(xiàn),染毒24 h時,各劑量組A549細(xì)胞凋亡率與對照組比較差異無統(tǒng)計學(xué)意義(P0.05),而各劑量組HepG2細(xì)胞凋亡率均顯著增加(P0.05或P0.01);染毒48 h時,≥40μg/mL劑量組A549細(xì)胞和160μg/mL劑量組HepG2細(xì)胞凋亡率均高于對照組(P0.05或P0.01)。使用NAC預(yù)處理細(xì)胞后,160μg/mL劑量組兩種細(xì)胞系凋亡率均顯著下降(P0.01)。結(jié)論:納米銀可以引起A549和HepG2細(xì)胞表現(xiàn)不同程度的增殖抑制和凋亡,而細(xì)胞內(nèi)不同的SOD和GSH改變可能是納米銀引起兩種細(xì)胞系不同毒作用的原因之一。
[Abstract]:Aim: to study the effects of silver nanoparticles on proliferation and apoptosis of human lung cancer (A549) and human hepatocellular carcinoma (HepG2) cell lines, and to explore the difference and mechanism of the toxic effects of silver nanoparticles on the two cell lines. Methods: A549 and HepG2 cells were inoculated with silver nanoparticles (20: 40, 80160, 320640 渭 g/mL) for 24 and 48 hours, respectively. The survival rate of A549 and HepG2 cells was detected by MTT method, and the cell culture medium was used as control. Glutathione (GSH) and superoxide dismutase (SOD) kit were used to detect GSH content and SOD activity. All the cells were treated with N-acetylcysteine (NAC) pretreated with 160 渭 g/mL silver nanoparticles. Flow cytometry was used to detect the apoptosis rate in each group. Results: compared with the control group, the survival rate of A549 cells and HepG2 cells in the group of 鈮，

本文編號：2310350