【摘要】：[目的]探討不同濃度鎘致人胚胎腎細(xì)胞(HEK293細(xì)胞)中微小RNA(miRNA)-21(miR-21)表達(dá)的改變。[方法]培養(yǎng)HEK293細(xì)胞,細(xì)胞經(jīng)0、30、60、120μmol/L CdCl_2染毒1、3、6、12 h后,MTT法測(cè)定細(xì)胞生長(zhǎng)情況,q RT-PCR檢測(cè)miR-21表達(dá)量。[結(jié)果]隨著CdCl_2濃度的增高,作用時(shí)間的延長(zhǎng),細(xì)胞的生長(zhǎng)受到抑制。在CdCl_2濃度為120μmol/L,染毒時(shí)間為12 h時(shí)抑制率達(dá)到最大,此時(shí)細(xì)胞生存率為8.57%。當(dāng)作用時(shí)間分別為1、3、6、12 h時(shí),不同濃度CdCl_2染毒的細(xì)胞中miR-21的表達(dá)量差別有統(tǒng)計(jì)學(xué)意義(均P0.01),且miR-21的表達(dá)量隨著CdCl_2染毒濃度的增加而增加(均P trend0.01);當(dāng)CdCl_2濃度分別為30、60、120μmol/L時(shí),作用不同時(shí)間,細(xì)胞中miR-21表達(dá)量差別也有統(tǒng)計(jì)學(xué)意義(均P0.05);在CdCl_2濃度為30μmol/L和120μmol/L時(shí),miR-21表達(dá)量隨CdCl_2作用時(shí)間的延長(zhǎng)而增高(P trend=0.030,P trend=0.011);在CdCl_2濃度為120μmol/L且染毒時(shí)間為12 h時(shí),miR-21表達(dá)量最大,為27.77。[結(jié)論]鎘染毒可導(dǎo)致HEK293細(xì)胞miR-21表達(dá)上調(diào)。
[Abstract]:[objective] to investigate the changes of RNA (miRNA)-21 (miR-21) expression in human embryonic kidney cells (HEK293 cells) induced by cadmium at different concentrations. [methods] HEK293 cells were cultured, and the cells were exposed to 0 030U 60120 渭 mol/L CdCl_2 for 12 h. The cell growth was measured by MTT and the expression of miR-21 was detected by Q RT-PCR. [results] with the increase of CdCl_2 concentration and the prolongation of action time, cell growth was inhibited. When the concentration of CdCl_2 was 120 渭 mol/L, for 12 h, the inhibition rate was the highest, and the cell survival rate was 8.57%. The expression of miR-21 in the cells exposed to different concentrations of CdCl_2 was significantly different (P0.01) when the time of treatment was 1 ~ 3 ~ 6 ~ 6 ~ (-1) h, respectively (P0.01), and the expression of miR-21 in the cells exposed to different concentrations of CdCl_2 was significantly different (P0.01). The expression of miR-21 increased with the increase of CdCl_2 concentration (all P trend0.01). When the concentration of CdCl_2 was 30 渭 mol/L and 60120 渭 mol/L, the expression of miR-21 in the cells was significantly different at different time (P0.05). When the concentration of CdCl_2 was 30 渭 mol/L and 120 渭 mol/L, the expression of miR-21 increased with the prolongation of CdCl_2 (P trend=0.030,P trend=0.011). When the concentration of CdCl_2 was 120 渭 mol/L and the exposure time was 12 h, the maximum expression of miR-21 was 27.77. [conclusion] cadmium exposure can induce up-regulation of miR-21 expression in HEK293 cells.
【作者單位】： 山西醫(yī)科大學(xué)公共衛(wèi)生學(xué)院流行病學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(編號(hào):81273040) 山西省回國(guó)留學(xué)人員科研資助項(xiàng)目(編號(hào):2015-055)
【分類號(hào)】：R114

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本文編號(hào)：2306162