【摘要】：目的：觀察鉛暴露對大鼠心血管細胞的增殖、凋亡的影響，探討鉛對心血管細胞增殖和凋亡影響的分子機制。 方法：選取健康，3-4周齡的SD大鼠52只，體重在99-114g。使用隨機數(shù)字法將SD大鼠分為正常對照組和實驗組，實驗組飲用1％的乙酸鉛水，正常對照組只飲用自來水，12天后處死正常對照組和實驗組各13只，取心臟和血管組織備用。40天后處死正常對照組和實驗組各13只，取心臟和血管組織備用。提取組織細胞總蛋白和核蛋白，使用Westernblot法檢測鉛暴露對心血管細胞增殖、凋亡和組蛋白乙酰化的變化情況。使用用凝膠圖象分析系統(tǒng)進行X光片掃描，法國Vilber Lourmat公司的Bio ID數(shù)碼成像分析軟件對Western印跡結(jié)果進行定量分析。數(shù)據(jù)使用SPSS13.0統(tǒng)計軟件進行數(shù)據(jù)分析，應(yīng)用t檢驗分析。 結(jié)果： 1鉛暴露不同時間大鼠心臟組織細胞增殖核抗原PCNA、凋亡相關(guān)基因Bcl-2和Bax以及組蛋白H3乙�；谋磉_變化 Western blot和計算機western blot圖像半定量分析結(jié)果顯示，與正常對照組比較，鉛暴露12天和40天的大鼠心臟組織細胞中增殖蛋白PCNA的表達明顯增加，差異具有統(tǒng)計學(xué)意義。鉛暴露12天后SD大鼠心臟組織細胞中的Bcl-2蛋白表達量明顯增加，Bax蛋白表達增加；鉛暴露喂養(yǎng)40天后的SD大鼠心臟組織細胞中Bcl-2蛋白表達量明顯減少，Bax蛋白表達增加，差異均具有統(tǒng)計學(xué)意義。鉛暴露12天和40天的大鼠心臟組織細胞中組蛋白H3乙�；谋磉_明顯增加，具有統(tǒng)計學(xué)差異。 2鉛暴露不同時間的大鼠主動脈血管細胞中增殖核抗原PCNA、凋亡相關(guān)基因Bcl-2和Bax以及組蛋白H3乙�；谋磉_變化 Western blot和計算機western blot圖像半定量分析結(jié)果顯示，與正常對照組比較，鉛暴露12天和40天的后大鼠主動脈血管細胞中的PCNA蛋白表達量明顯減少，差異具有明顯的統(tǒng)計學(xué)意義。鉛暴露12天的SD大鼠主動脈血管細胞中Bcl-2蛋白表達量明顯減少，Bax蛋白表達量減少；鉛暴露40天的SD大鼠主動脈血管細胞中Bcl-2蛋白表達量減少，Bax蛋白的表達量增加，差異均具有統(tǒng)計學(xué)意義。鉛暴露12天和40天后的大鼠主動脈血管細胞中乙�；慕M蛋白H3表達量明顯增加，具有統(tǒng)計學(xué)差異。 結(jié)論： 1鉛暴露使心臟組織細胞出現(xiàn)增殖、凋亡和組蛋白H3乙酰化水平的升高，，提示鉛暴露過程中組蛋白H3乙酰化在心臟組織細胞的增殖和凋亡中起著重要作用。 2鉛暴露抑制了血管組織細胞的增殖，促進了血管組織細胞的凋亡和組蛋白H3的乙�；f明鉛暴露的過程中組蛋白H3乙�；谘芙M織細胞的增殖和凋亡過程中起著重要作用。
[Abstract]:Aim: to observe the effects of lead exposure on the proliferation and apoptosis of cardiovascular cells in rats and to explore the molecular mechanism of the effects of lead on the proliferation and apoptosis of cardiovascular cells. Methods: 52 healthy 3-4-week old SD rats with body weight of 99-114 g were selected. SD rats were randomly divided into control group and experimental group. The experimental group drank 1% lead acetate water, and the normal control group only drank tap water. After 12 days, 13 normal control group and 13 experimental group were killed. After 40 days, the normal control group and the experimental group were killed. Total protein and nuclear protein were extracted and the changes of proliferation apoptosis and histone acetylation of cardiovascular cells exposed to lead were detected by Westernblot method. The gel image analysis system was used to scan X-ray film and the Bio ID digital imaging analysis software of Vilber Lourmat Company of France was used to quantitatively analyze the results of Western blotting. The data are analyzed by SPSS13.0 statistical software and t-test. Results: 1 the changes of expression of apoptosis-related genes Bcl-2 and Bax and histone H3 acetylation of proliferating nuclear antigen (PCNA,) in rat heart tissue at different time after exposure to lead were detected by semi-quantitative analysis of Western blot and western blot images. Compared with the control group, the expression of proliferating protein PCNA in cardiac tissue cells of rats exposed to lead for 12 days and 40 days was significantly increased, and the difference was statistically significant. After 12 days of lead exposure, the expression of Bcl-2 protein and Bax protein in cardiac tissue cells of SD rats were significantly increased. After 40 days of lead exposure, the expression of Bcl-2 protein decreased significantly and the expression of Bax protein increased in the heart of SD rats. The difference was statistically significant. The expression of histone H 3 acetylated in cardiac tissue cells of rats exposed to lead for 12 days and 40 days was significantly increased with statistical difference. 2 the changes of expression of proliferating nuclear antigen PCNA, apoptosis-related genes Bcl-2 and Bax and histone H3 acetylation in rat aortic vascular cells exposed to lead at different time. The results of semi-quantitative analysis of Western blot and computer western blot images showed that: 1. Compared with the control group, the expression of PCNA protein in aortic vascular cells of rats exposed to lead for 12 days and 40 days was significantly decreased, and the difference was statistically significant. The expression of Bcl-2 protein and Bax protein in aortic vascular cells of SD rats exposed to lead for 12 days decreased significantly. After 40 days of lead exposure, the expression of Bcl-2 protein decreased and the expression of Bax protein increased in aortic vascular cells of SD rats. The expression of acetylated histone H3 in aortic vascular cells of rats exposed to lead for 12 days and 40 days was significantly increased with statistical difference. Conclusion: 1 lead exposure induced proliferation, apoptosis and histone H _ 3 acetylation, suggesting that histone H _ 3 acetylation plays an important role in the proliferation and apoptosis of cardiac tissue cells during lead exposure. 2 lead exposure inhibited the proliferation of vascular tissue cells, promoted the apoptosis of vascular tissue cells and the acetylation of histone H3. These results suggest that histone H 3 acetylation plays an important role in the proliferation and apoptosis of vascular tissue cells during lead exposure.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R114

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