【摘要】：目的:探討表沒食子兒茶素沒食子酸酯(EGCG)和維生素E(Vit E)對鉛暴露仔鼠學(xué)習(xí)記憶能力的影響。方法:將健康SPF級昆明雌鼠隨機(jī)分為對照組(4只)和染毒組(20只)。染毒組飲2 g/L醋酸鉛溶液染毒21 d,對照組飲無鉛去離子水21 d。染毒結(jié)束后按雌雄比2∶1合籠飼養(yǎng)。分娩后第21天,將染毒組所產(chǎn)仔鼠隨機(jī)分為7組,即生理鹽水組、玉米油組、EGCG干預(yù)組(3.0 mg/kg)、VitE干預(yù)組(30 IU/kg)、聯(lián)合干預(yù)1組(EGCG 3.0 mg/kg+VitE 30 IU/kg)、聯(lián)合干預(yù)2組(EGCG 1.5 mg/kg+VitE 60 IU/kg)、聯(lián)合干預(yù)3組(EGCG 6.0 mg/kg+VitE 15IU/kg),每組10只;隨機(jī)選取10只對照組雌鼠所產(chǎn)仔鼠作為正常對照組。正常對照組仔鼠飲無鉛去離子水,其余7組仔鼠灌胃相應(yīng)試劑,共干預(yù)28 d。灌胃結(jié)束后,用Morris水迷宮測定小鼠學(xué)習(xí)記憶能力,用極譜儀測定小鼠血鉛含量,用氧化酶試劑盒測定海馬組織中SOD活性和MDA含量。結(jié)果:與正常對照組相比,生理鹽水組和玉米油組逃避潛伏期延長,穿越平臺次數(shù)減少,血鉛含量升高,海馬組織中SOD活性降低,MDA含量增加(P0.05)。與生理鹽水組和玉米油組相比,各干預(yù)組逃避潛伏期縮短,穿越平臺次數(shù)增多,血鉛含量不同程度降低,聯(lián)合干預(yù)1、3組SOD活性升高,MDA含量降低(P0.05)。結(jié)論:EGCG和VitE可能通過提高海馬組織中SOD活性,改善仔鼠的學(xué)習(xí)記憶能力,對鉛暴露仔鼠神經(jīng)損傷有一定的保護(hù)作用。
[Abstract]:Aim: to investigate the effects of epigallocatechin gallate (EGCG) and vitamin E (Vit E) on learning and memory in lead exposed rats. Methods: healthy SPF female mice were randomly divided into control group (n = 4) and exposure group (n = 20). The exposure group drank 2 g / L lead acetate solution for 21 days, while the control group drank lead-free deionized water for 21 days. After exposure to the end of the male and female according to 2:1 cage feeding. On the 21st day after delivery, the rats in the exposed group were randomly divided into 7 groups, that is, the saline group. Corn oil group, EGCG intervention group (30 IU/kg), combined intervention group 1 (EGCG 3.0 mg/kg VitE 30 IU/kg), combined intervention group 2 (EGCG 1.5 mg/kg VitE 60 IU/kg), combined intervention group (EGCG 6.0 mg/kg VitE 15IU/kg), 10 rats in each group; Ten female rats of control group were randomly selected as normal control group. The rats in the control group drank lead-free deionized water, and the other 7 rats were treated with corresponding reagents for 28 days. The learning and memory ability of mice was measured by Morris water maze, the blood lead content was measured by polarography, the activity of SOD and the content of MDA in hippocampal tissue were measured by oxidase kit. Results: compared with the normal control group, the escape latency of normal saline group and corn oil group was prolonged, the times of crossing the plateau decreased, the blood lead content increased, the activity of SOD and the content of MDA in hippocampus decreased (P0.05). Compared with normal saline group and corn oil group, the escape latency of each intervention group was shorter, the number of crossing platform increased, the blood lead content decreased in varying degrees, the activity of SOD increased and the content of MDA decreased in the combined intervention group 1: 3 (P0.05). Conclusion: EGCG and VitE may enhance the activity of SOD in hippocampus and improve the ability of learning and memory of newborn rats, which may have a protective effect on the nerve injury of rats exposed to lead.
【作者單位】： 鄭州大學(xué)公共衛(wèi)生學(xué)院營養(yǎng)與食品衛(wèi)生學(xué)教研室;新鄉(xiāng)醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金資助項目81172716
【分類號】：R114

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