【摘要】：目的觀察補體片段C3f對人胚肺成纖維細胞(MRC-5)合成α-平滑肌肌動蛋白(α-SMA)的影響。方法將低中高3個不同質(zhì)量濃度(15.6,62.5,250 ng·mL-1)人工合成的補體片段C3f(實驗組)加入培養(yǎng)基中,以刺激MRC-5細胞,以細胞免疫組化法檢測α-SMA在MRC-5細胞質(zhì)中的表達情況。同時,設立對照組:10 ng·mL~(-1)轉(zhuǎn)化生長因子-β_1(TGF-β_1)和空白組(濃度為0的C3f),進行對比研究。結(jié)果中劑量實驗組與對照組(TGF-β_1)刺激MRC-5細胞合成α-SMA量分別為7.46±0.66,7.54±0.51,差異無統(tǒng)計學意義(P0.05),說明2組的量相當,表明C3f具有刺激MRC-5合成α-SMA的功能。低中高3個不同質(zhì)量濃度的細胞內(nèi)α-SMA表達量分別為4.95±0.78,7.46±0.66和9.23±0.21,顯示C3f的濃度與α-SMA的表達量呈正相關(guān)(P0.05),表明C3f具有促進MRC-5表達α-SMA的功能。結(jié)論補體片段C3f能夠刺激MRC-5細胞內(nèi)α-SMA的合成增加。
[Abstract]:Objective to observe the effect of complement fragment C 3f on the synthesis of 偽 -smooth muscle actin (偽-SMA) in human embryonic lung fibroblasts (MRC-5). Methods three synthetic complement fragments C3f (experimental group) of low, medium and high concentration (15.6n 62.5250 ng mL-1) were added to the medium to stimulate MRC-5 cells, and the expression of 偽 SMA in the cytoplasm of MRC-5 was detected by immunohistochemical method. At the same time, 10 ng mL~ (-1) transforming growth factor- 尾 _ 1 (TGF- 尾 _ 1) and control group (C _ 3f with concentration of 0) were set up for comparative study. Results the amount of 偽-SMA stimulated by TGF- 尾 _ 1 in the middle dose group and the control group was 7.46 鹵0.666 鹵7.54 鹵0.51, respectively. There was no significant difference between the two groups (P0.05), indicating that C3f had the function of stimulating MRC-5 to synthesize 偽 -SMA. The expression levels of 偽-SMA were 4.95 鹵0.78 鹵7.46 鹵0.66 and 9.23 鹵0.21, respectively, indicating that C3f was positively correlated with the expression of 偽-SMA (P0.05), indicating that C3f could promote the expression of 偽-SMA by MRC-5. Conclusion C3f can stimulate the synthesis of 偽-SMA in MRC-5 cells.
【作者單位】： 天津市第二人民醫(yī)院檢驗科天津市肝病研究所;北京大學濱海醫(yī)院檢驗科;天津市東麗區(qū)東麗醫(yī)院檢驗科;武警后勤學院醫(yī)學院部隊流行病學與統(tǒng)計學教研室;天津市西青區(qū)西青醫(yī)院;
【基金】：國家自然科學基金面上基金資助項目(30771788) 天津市衛(wèi)生局科技基金資助項目(06KG10) 天津市應用基礎研究計劃面上基金資助項目(06YFJMJC09900)
【分類號】：R135.2
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本文編號：2293166