【摘要】：建立用于測定乳粉、綠豆糕、豬肉、熏魚肉、調(diào)味粉中白菖蒲油主要成分α-細(xì)辛醚和β-細(xì)辛醚含量的固相萃取-高效液相色譜分析方法。選擇乙腈提取樣品中的細(xì)辛醚,提取液經(jīng)硅膠固相萃取小柱凈化,采用配備熒光檢測器的高效液相色譜儀進(jìn)行分離測定,外標(biāo)法定量。乳粉、綠豆糕、豬肉、熏魚肉、調(diào)味粉中α-細(xì)辛醚和β-細(xì)辛醚的方法定量限均為0.01 mg/kg,在0.01~0.50 mg/kg的添加水平范圍內(nèi),α-細(xì)辛醚的平均回收率在78.2%~99.4%之間,β-細(xì)辛醚的平均回收率在80.8%~105.6%之間,相對標(biāo)準(zhǔn)偏差為1.6%~10.3%。該方法能夠滿足食品中細(xì)辛醚的痕量檢測要求。
[Abstract]:A solid phase extraction-HPLC method was developed for the determination of 偽 -asarone and 尾 -asarone in milk powder, mung bean cake, pork, smoked fish meat and seasoning powder. Acetonitrile was selected to extract asarone from the sample. The extract was purified by silica gel solid-phase extraction column and separated and determined by high performance liquid chromatography equipped with fluorescence detector. The quantitative limits of 偽 -asarone and 尾 -asarone in milk powder, mung bean cake, pork, smoked fish and seasoning powder were 0.01 mg/kg, and 0.01 mg/kg respectively. The average recovery of 偽 -asarone was between 78.2% and 99.4%, and the average recovery of 尾 -asarone was between 80.8% and 105.6%. The relative standard deviation was 1.6 / 10.3. The method can meet the requirements of trace determination of asarone in food.
【作者單位】： 衢州出入境檢驗(yàn)檢疫局;義烏出入境檢驗(yàn)檢疫局;
【基金】：國家出入境檢驗(yàn)檢疫行業(yè)標(biāo)準(zhǔn)制修訂項目(2009B527)
【分類號】：R155.5
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本文編號：2289529