【摘要】：人源諾如病毒(Human noroviruses, HuNoVs)是引起人類非細(xì)菌性腸胃炎的主要病原。果蔬(如生菜、草莓類)表面可吸附HuNoVs,是其重要傳播的載體之一。但是,鮮見果蔬中病毒受體、病毒傳播等與污染和傳播機(jī)制相關(guān)的信息。因此,探索果蔬中HuNoVs的分布規(guī)律,分析HuNoVs受體及其性質(zhì),是揭示果蔬傳播HuNoVs機(jī)制的突破口。在前期工作的基礎(chǔ)上,本研究以表面展示HuNoVs (GⅠ.1/GⅡ.4)衣殼蛋白P結(jié)構(gòu)域的大腸桿菌[INP-P(GⅠ.1/GⅡ.4)BL21]為HuNoVs替代物,以激光共聚焦熒光顯微鏡,分析了生菜表面HuNoVs受體的結(jié)合和分布情況;并以ELISA方法,對(duì)生菜葉提取物中病毒受體類型及性質(zhì)做了初步分析。結(jié)果如下:(1)以激光共聚焦熒光顯微鏡分析生菜葉面吸附INP-P(GⅠ.1/GⅡ.4)BL21的規(guī)律。結(jié)果發(fā)現(xiàn):老生菜葉的葉面、葉脈和氣孔處易吸附HuNoVs;相應(yīng)地,新葉吸附病毒能力較差。(2) ELISA對(duì)生菜葉提取液中病毒類受體進(jìn)行檢測,結(jié)果發(fā)現(xiàn):唾液和PGM處理可以特異性抑制INP-P(GⅠ.1/GⅡ.4)BL21與生菜葉提取物的結(jié)合;NaIO4可以顯著性抑制INP-P(GⅠ.1/GⅡ.4)BL21同生菜葉提取液的結(jié)合。生菜葉提取物煮沸后,可部分抑制與INP-P(GⅡ.4)BL21的結(jié)合。因此,HuNoVs與生菜葉提取物結(jié)合的介質(zhì)為類HBGAs糖蛋白,且結(jié)合的主要成分為多糖基團(tuán);GⅡ.4型HuNoV可特異性結(jié)合生菜葉提取物中的蛋白質(zhì),但GⅠ.1型HuNoV沒有該現(xiàn)象。(3) pH對(duì)HuNoVs同生菜的結(jié)合影響小。離子間力和疏水作用在HuNoVs與生菜互作過程中有重要作用。
[Abstract]:Human norovirus (Human noroviruses, HuNoVs) is the main pathogen of human non-bacterial gastroenteritis. The adsorbable HuNoVs, on the surface of fruits and vegetables (such as lettuce, strawberry) is one of the important carriers. However, virus receptors and virus transmission in fruits and vegetables are rarely known to be related to pollution and transmission mechanisms. Therefore, to explore the distribution of HuNoVs in fruits and vegetables, to analyze the HuNoVs receptor and its properties is a breakthrough to reveal the mechanism of spreading HuNoVs in fruits and vegetables. On the basis of previous work, we used INP-P (G 鈪，

本文編號(hào)：2270946