【摘要】：目的觀察L02細(xì)胞經(jīng)飲用水有機(jī)提取物染毒后所誘發(fā)的白介素6(interleukin 6,IL-6)和丙氨酸氨基轉(zhuǎn)移酶(alanine transaminase,ALT)及細(xì)胞凋亡的改變。方法采用固相萃取法提取飲用水中有機(jī)污染物。將取處于對數(shù)生長期的正常人肝(L02)細(xì)胞分別暴露于分別暴露于含終濃度0.312 5、0.625 0、1.250 0、2.500 0、5.000 0 L/ml有機(jī)提取物的培養(yǎng)液中暴露24、48、72 h,同時(shí)設(shè)溶劑對照(含0.1%DMSO培養(yǎng)液)和空白對照(培養(yǎng)液)。檢測細(xì)胞凋亡率及細(xì)胞培養(yǎng)液上清中IL-6的含量和ALT的活力。結(jié)果與溶劑對照組比較,各劑量飲用水有機(jī)提取物染毒不同時(shí)間L02細(xì)胞的凋亡率均較高,除0.312 5、0.625 0 L/ml染毒24 h和0.312 5 L/ml染毒48 h組外,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);各劑量飲用水有機(jī)提取物染毒不同時(shí)間L02細(xì)胞培養(yǎng)液上清中IL-6的含量均較高,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);各劑量飲用水有機(jī)提取物染毒不同時(shí)間L02細(xì)胞培養(yǎng)液上清中ALT的活力均較高,除0.312 5、0.625 0 L/ml染毒24 h組外,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。且隨著飲用水有機(jī)提取物染毒劑量的升高和染毒時(shí)間的延長,L02細(xì)胞的凋亡率及細(xì)胞培養(yǎng)液上清中IL-6含量和ALT活力均呈上升趨勢。結(jié)論在本實(shí)驗(yàn)條件下,飲用水有機(jī)污染物可劑量依賴性及時(shí)間依賴性地誘導(dǎo)L02細(xì)胞炎性因子IL-6的分泌,引發(fā)炎癥反應(yīng),誘導(dǎo)細(xì)胞凋亡,從而導(dǎo)致肝細(xì)胞損傷。
[Abstract]:Objective to observe the changes of interleukin 6 (interleukin 6), alanine aminotransferase (alanine transaminase,ALT) and apoptosis induced by organic extracts from drinking water in L02 cells. Methods solid phase extraction was used to extract organic pollutants from drinking water. The normal human liver (L02) cells at logarithmic growth stage were exposed to the medium containing the final concentration of 0.312 ~ 0.625 ~ 1.2500 ~ 2.500 ~ 5.000 L/ml for 24 ~ 4872 h, respectively. The cells were exposed to a solvent control (including 0.1%DMSO medium) and a blank control (culture medium) respectively. Apoptosis rate, IL-6 content and ALT activity in supernatant of cell culture medium were measured. Results compared with the solvent control group, the apoptosis rate of L02 cells was higher at different time after exposure to organic extracts from drinking water at different time points, except for the groups exposed for 24 h and 0.312 5 L/ml, respectively. The content of IL-6 in the supernatant of L02 cells was higher at different time after exposure to organic extracts from drinking water (P0.05). The activity of ALT in the supernatant of L02 cells exposed to different doses of drinking water organic extract at different time was higher than that of the control group (P 0.05), except for the treatment group of 0.312 ~ 0.625 L/ml for 24 h (P0.05). The apoptosis rate, IL-6 content and ALT activity in the supernatant of L02 cells were increased with the increase of drinking water organic extract dose and the prolongation of exposure time. Conclusion in this experiment, drinking water organic pollutants can induce IL-6 secretion of L02 cells in a dose-and time-dependent manner, induce inflammatory reaction, induce apoptosis and lead to hepatocyte damage.
【作者單位】： 貴州醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;
【基金】：貴州省科技廳基金(黔科合LH字[2014]7102) 貴陽市科學(xué)技術(shù)計(jì)劃項(xiàng)目(筑科合同[2013103]19號(hào))
【分類號(hào)】：R114

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