【摘要】：本研究旨在探討甲醛導(dǎo)致機體神經(jīng)系統(tǒng)病變的具體機制。選用雄性Balb/c小鼠為研究對象,動態(tài)吸入甲醛方式染毒7天,每天8 h,甲醛濃度分別為0、0.5、3.0 mg/m3,同時設(shè)置一氧化氮合酶(nitric oxide synthase,NOS)拮抗劑(NG-monomethylL-arginine,L-NMMA)組,該組小鼠同時進行3.0 mg/m3甲醛染毒。染毒結(jié)束后,用試劑盒檢測小鼠大腦皮層、海馬和腦干中環(huán)磷酸腺苷(cyclic adenosine monophosphate,cAMP)、環(huán)磷酸鳥苷(cyclic guanine monophosphate,cGMP)、一氧化氮(nitric oxide,NO)含量和NOS活性的變化。結(jié)果顯示,與對照組相比,小鼠大腦皮層和腦干中cAMP含量在0.5 mg/m3染毒組顯著升高(均P0.05),但是在3.0 mg/m3染毒組顯著降低(P0.05),海馬中cAMP含量僅在3.0 mg/m3染毒組出現(xiàn)顯著降低(P0.05);與對照組相比,L-NMMA拮抗組小鼠cGMP和NO含量分別在海馬和大腦皮層中顯著上升(均P0.01),而cAMP含量和NOS活性在不同腦區(qū)中無顯著變化。與3.0 mg/m3染毒組相比,L-NMMA拮抗組不同腦區(qū)中cAMP含量均顯著上升(均P0.05),NOS活性顯著下降(P0.05或P0.01);大腦皮層和腦干中的cGMP含量以及腦干中的NO含量亦出現(xiàn)顯著性變化(P0.05或P0.01)。以上結(jié)果提示,甲醛暴露的神經(jīng)系統(tǒng)毒性作用與NO/cGMP信號轉(zhuǎn)導(dǎo)通路和cAMP信號通路存在一定的關(guān)系。
[Abstract]:The purpose of this study was to explore the mechanism of formaldehyde causing neuropathy. Male Balb/c mice were exposed to formaldehyde for 7 days and 8 hours a day. The concentration of formaldehyde was 0.5 mg/m3, and 3.0 mg/m3, respectively. The mice were treated with (nitric oxide synthase,NOS antagonist (NG-monomethylL-arginine,L-NMMA). The mice were exposed to 3.0 mg/m3 formaldehyde at the same time. After exposure, the changes of (cyclic adenosine monophosphate,cAMP, (cyclic guanine monophosphate,cGMP, nitric oxide,NO and NOS activity in cerebral cortex, hippocampus and brainstem of mice were detected by using the kit. The contents of cyclic guanosine monophosphate (cyclic guanine monophosphate,cGMP) and the content of cyclic adenosine monophosphate (nitric oxide,NO) in hippocampus and brain stem were measured. The results showed that, compared with the control group, The content of cAMP in cerebral cortex and brainstem of mice increased significantly at 0.5 mg/m3 (P0.05), but decreased significantly in 3.0 mg/m3 group (P0.05), and cAMP content in hippocampus decreased only in 3.0 mg/m3 group (P0.05). Compared with control group, L-NMMA antagonistic group was smaller than control group. The contents of cGMP and NO increased significantly in hippocampus and cerebral cortex respectively (P0.01), but the content of cAMP and the activity of NOS did not change in different brain regions. Compared with 3.0 mg/m3 group, cAMP content in different brain regions of L-NMMA antagonistic group increased significantly (P0.05 or P0.01), and cGMP content in cerebral cortex and brainstem and NO content in brainstem also showed significant changes (P0.05 or P0.01). These results suggest that the neurotoxicity of formaldehyde exposure is related to NO/cGMP signal transduction pathway and cAMP signaling pathway.
【作者單位】： 華中師范大學(xué)生命科學(xué)學(xué)院遺傳調(diào)控與整合生物學(xué)湖北省重點實驗室;
【基金】：supported by the Key Project of National Natural Science Foundation of China(No.51136002) the Open Project Program of Hubei Key Laboratory of Genetic Regulation and Integrative Biology,China(No.GRIB201501)
【分類號】：R114

【參考文獻】

相關(guān)期刊論文 前1條

1 陳立軍;張廷模;彭成;;大黃不同炮制品對大鼠一般狀況及血漿cAMP、cGMP含量的影響[J];成都中醫(yī)藥大學(xué)學(xué)報;2009年04期

【共引文獻】

相關(guān)期刊論文 前9條

1 秦承偉;張勇;趙彥明;高桃;張如意;張勵才;;中縫背核參與大鼠嗎啡依賴和戒斷的形成機制研究[J];中國臨床藥理學(xué)雜志;2011年10期

2 張黎;李娜;梁明輝;王春華;劉特;葉琳;;甲醛對小鼠認知與運動功能的影響及其機制[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2012年05期

3 石雪;鮑萍;蔣紅;楊敬宗;;甲醛的危害和法規(guī)要求[J];現(xiàn)代絲綢科學(xué)與技術(shù);2013年05期

4 閆丹丹;白劍英;梁瑞峰;王幼萍;雷佩玉;;甲醛對HepG2細胞脂肪代謝的影響[J];癌變.畸變.突變;2013年06期

5 顧誠;陳志剛;劉成寶;馬娟寧;;改性膨脹石墨基炭/炭復(fù)合材料對甲醛氣體的吸附性能[J];機械工程材料;2013年12期

6 余敏;陶玲;錢志余;朱威州;;基于彌散張量成像的腦結(jié)構(gòu)網(wǎng)絡(luò)參數(shù)研究[J];北京生物醫(yī)學(xué)工程;2014年05期

7 Xiao-Liang ZHANG;Wei PANG;Xin-Tian HU;Jia-Li LI;Yong-Gang YAO;Yong-Tang ZHENG;;Experimental primates and non-human primate(NHP) models of human diseases in China: current status and progress[J];動物學(xué)研究;2014年06期

8 袁勤;黎源;譚波;;基于似然同步方法的非線性腦電信號分析方法[J];電子科技大學(xué)學(xué)報;2015年02期

9 孫文淵;呂世明;譚艾娟;林艷紅;安苗;華夏;李博巖;焦亞琴;;丁香酚對鯉魚腦和脊髓中鈉鉀泵活性的抑制效果[J];貴州農(nóng)業(yè)科學(xué);2015年06期

【二級參考文獻】

相關(guān)期刊論文 前5條

1 武桂新,王楓,吳云娥,馮煒權(quán);運動與骨骼肌內(nèi)一氧化氮[J];北京體育大學(xué)學(xué)報;1999年03期

2 王振綱,劉淑英,盧琦華,李樾明,劉景生,孫燕,史濟招;中醫(yī)辨證與血漿環(huán)磷酸腺苷(cAMP)含量的關(guān)系[J];北京醫(yī)學(xué);1979年02期

3 熊正英,曲洪剛;一氧化氮與運動疲勞關(guān)系的研究進展[J];福建體育科技;2004年02期

4 于立群;甲醛的健康效應(yīng)[J];國外醫(yī)學(xué)(衛(wèi)生學(xué)分冊);2004年02期

5 趙軍,林文_",翁錫全;間歇性低氧對訓(xùn)練大鼠血清NO、NOS及自由基代謝的影響[J];廣州體育學(xué)院學(xué)報;2004年04期

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