【摘要】：腸道屏障作為機體防御外部致病原的第一道防線具有重要的作用,斷奶幼齡動物由于受心理、環(huán)境、營養(yǎng)等因素的影響,斷奶應(yīng)激會改變消化道結(jié)構(gòu)和內(nèi)部微生態(tài)環(huán)境,進而破壞腸道屏障功能,增加腸道通透性,引起炎癥性腹瀉,影響斷奶幼齡動物正常的生長發(fā)育,甚至危及生命。本實驗首先研究低聚乳果糖不同劑量對斷奶大鼠在不同時間段內(nèi)的生長性能的影響,隨后以脂多糖刺激斷奶大鼠模擬斷奶幼齡動物生病前的機體環(huán)境,研究斷奶大鼠在低聚乳果糖預(yù)前干預(yù)的情況下的血清中生化指標、大便理化性質(zhì)、腸道內(nèi)部微環(huán)境和形態(tài)、腸道通透性、腸道黏膜中細胞因子水平及上皮細胞緊密連接蛋白表達的變化。以期揭示不同劑量低聚乳果糖調(diào)控斷奶動物腸道緊密連接及屏障功能的機制。同時可為研究低聚乳果糖應(yīng)用于嬰兒食品配方提供科學(xué)的參考依據(jù)。 實驗結(jié)果表明,在第2周的時間內(nèi),低聚乳果糖才對斷奶大鼠生長性能發(fā)揮了有益作用,表現(xiàn)為體質(zhì)量有增加的趨勢(p0.05),料重比顯著下降(p0.05)。脂多糖應(yīng)激后,相對于陽性斷奶大鼠,低聚乳果糖能顯著增加血清中IgM水平(p0.05),顯著降低血清中ALB(p0.05)、BUN(p0.05)、CK(p0.05)水平；顯著降低盲腸食糜中NH3-N水平(p0.05),增加食糜中乙酸和丙酸及大便中丁酸的含量(p0.05)；明顯改善大便形狀評分(p0.01),顯著減少大便次數(shù)(p0.05)；顯著增加空腸和回腸絨毛高度／隱窩深度比值(p0.05)、結(jié)腸黏液層厚度和上皮細胞間杯狀細胞數(shù)(p0.05)；顯著減少腸道通透性(p0.05),抑制血清中DAO活性,調(diào)節(jié)促炎性和抑炎性細胞因子的平衡,顯著增加斷奶大鼠結(jié)腸黏膜Occludin蛋白表達(p0.05)。 綜上所述,低聚乳果糖對斷奶大鼠生長性能有促進作用。通過抑制腸道黏膜萎縮,改善腸道微環(huán)境,增加腸道緊密連接蛋白的表達,降低腸道通透性,保護斷奶大鼠腸道屏障功能。
[Abstract]:Intestinal barrier plays an important role as the first line of defense against external pathogenic agents. Weaning young animals are affected by psychological, environmental and nutritional factors, and weaning stress will change the structure of digestive tract and internal microecological environment. Furthermore, the function of intestinal barrier is destroyed, intestinal permeability is increased, inflammatory diarrhea is caused, normal growth and development of weaning young animals are affected, and even life is endangered. The effects of different doses of oligosaccharide on the growth performance of weaned rats at different time periods were studied, and then lipopolysaccharide was used to stimulate weaning rats to simulate the organism environment before weaning young animals became ill. To study the serum biochemical indexes, stool physicochemical properties, intestinal microenvironment and morphology, and intestinal permeability of weaned rats after pre-intervention with oligosaccharide. Changes of cytokine levels and tight junction protein expression in intestinal mucosa. The aim of this study was to reveal the mechanism of regulation of tight intestinal junction and barrier function in weaned animals with different doses of oligosaccharide. At the same time, it can provide scientific reference for studying the application of oligosaccharide in infant food formulation. The results showed that during the second week, fructose-oligosaccharide had a beneficial effect on the growth performance of weaned rats, showing an increasing trend of body mass (p0.05) and a significant decrease in the ratio of feed to weight (p0.05). After lipopolysaccharide stress, fructose oligosaccharides significantly increased the level of IgM in serum and decreased the level of bun (p0.05) and CK (p0.05) in serum compared with positive weaned rats. The content of acetic acid and propionic acid and butyric acid in stool were significantly decreased (p0. 05), the scores of stool shape were improved (p0. 01), the number of defecation was significantly decreased (p0. 05), the content of acetic acid and propionic acid in chylum was increased significantly (p0. 05), and the score of stool shape was significantly improved (p0. 01). The ratio of height of jejunum and ileum villi to crypt depth (p0.05), the thickness of mucus layer of colon and the number of goblet cells between epithelial cells (p0.05) were significantly increased, the intestinal permeability was significantly decreased (p0.05), the activity of DAO in serum was inhibited, and the balance of pro-inflammatory and anti-inflammatory cytokines was regulated. The expression of Occludin protein in colon mucosa of weaned rats was significantly increased (p0.05). In conclusion, fructooligosaccharide can promote the growth performance of weaning rats. Through inhibiting intestinal mucosal atrophy, improving intestinal microenvironment, increasing the expression of intestinal tight junction protein, reducing intestinal permeability and protecting intestinal barrier function of weaning rats.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R151

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