【摘要】：[目的]建立一種快速有效地檢測(cè)水中微量戊二醛的酚試劑分光光度法。[方法]采用戊二醛與酚試劑(3-甲基-苯并噻唑腙,MBTH)反應(yīng)生成嗪,嗪在酸性溶液中能夠被高鐵離子氧化生成藍(lán)綠色化合物,利用產(chǎn)生的顏色與戊二醛含量成正比的原理,采用酚試劑分光光度法對(duì)水中微量戊二醛含量進(jìn)行測(cè)定,分析入射波長(zhǎng)、酚試劑濃度、顯色劑酸溶解條件、顯色溫度和顯色時(shí)間等因素對(duì)實(shí)驗(yàn)結(jié)果的影響并確定最佳條件。[結(jié)果]確定了604 nm處為最佳吸收波長(zhǎng);MBTH和硫酸高鐵銨的最佳用量分別為200 mg/L和1 800 mg/L;在30℃下放置15 min后MBTH與戊二醛能夠充分反應(yīng)生成嗪;其次研究了顯色劑酸溶解條件,其最佳鹽酸溶解用量為0.2 mol/L;最后研究得出最佳顯色溫度和顯色時(shí)間為40℃下靜置40 min。在最佳測(cè)試條件下,吸光度與戊二醛濃度在0.005~1.00 mg/L范圍內(nèi)呈現(xiàn)良好線(xiàn)性關(guān)系,檢測(cè)限為0.005 mg/L,相對(duì)標(biāo)準(zhǔn)偏差為0.34%,平均加標(biāo)回收率為98.13%。[結(jié)論]該方法檢測(cè)限低、靈敏度高、線(xiàn)性范圍寬,能夠快速有效地檢測(cè)水中微量戊二醛。
[Abstract]:[objective] to establish a rapid and effective spectrophotometric method for the determination of trace glutaraldehyde in water. [methods] the reaction of glutaraldehyde with phenol reagent (3-methyl-benzothiazolyl Hydrazone MBTH) was used to produce pyrazine, which could be oxidized by iron ion in acid solution to form blue-green compound. The color produced was proportional to the content of glutaraldehyde. The content of glutaraldehyde in water was determined by phenol reagent spectrophotometry. The influence of incident wavelength, concentration of phenol reagent, acid dissolution condition of chromogenic agent, color reaction temperature and color reaction time on the experimental results were analyzed and the optimum conditions were determined. [results] the optimum absorption wavelength at 604 nm was determined to be 200 mg/L and 1 800 mg/L;, respectively. MBTH and glutaraldehyde could fully react with glutaraldehyde at 30 鈩，

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