【摘要】：葉黃素和玉米黃質(zhì)是人類眼睛中主要的類胡蘿卜素,它們對預防眼部疾病具有重要作用。本文通過研究不同比例的葉黃素和玉米黃質(zhì)在體內(nèi)和體外的抗氧化活性,探討其協(xié)同抗氧化活性,為復合類胡蘿卜素產(chǎn)品的開發(fā)提供理論依據(jù)。通過DPPH自由基清除體系、FRAP法和ORAC法三種方法探討了葉黃素和玉米黃質(zhì)之間的體外協(xié)同抗氧化活性。通過建立ICR小鼠體內(nèi)乙醇氧化損傷模型,考察葉黃素和玉米黃質(zhì)在體內(nèi)的協(xié)同抗氧化活性。結(jié)果表明:葉黃素與玉米黃質(zhì)以1∶2比例存在時體外協(xié)同抗氧化效果最佳,2∶1效果較差。葉黃素/玉米黃質(zhì)(1∶2)組在各個指標上顯示出更強的體內(nèi)抗氧化活性。
[Abstract]:Lutein and zeaxanthin are the main carotenoids in human eyes, which play an important role in the prevention of eye diseases. In this paper, the antioxidant activities of lutein and zeaxanthin in different proportions in vivo and in vitro were studied, and the synergistic antioxidant activities were discussed, which provided a theoretical basis for the development of compound carotenoid products. The synergistic antioxidant activity between lutein and zeaxanthin was studied by DPPH radical scavenging system and ORAC method in vitro. The synergistic antioxidant activity of lutein and zeaxanthin in ICR mice was studied by establishing a model of ethanol oxidative damage in vivo. The results showed that the synergistic antioxidant effect of lutein and zeaxanthin at 1:2 in vitro was poor. Lutein / zeaxanthin (1:2) group showed stronger antioxidant activity in each index.
【作者單位】： 大連海洋大學食品科學與工程學院;大連醫(yī)諾生物有限公司;
【基金】：大連市企業(yè)博士后研究人員資助項目
【分類號】：O629.4;R151

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本文編號：2154007