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長(zhǎng)江武漢段地表水指示微生物與致病菌相關(guān)關(guān)系的研究與分析

發(fā)布時(shí)間:2018-06-24 15:17

  本文選題:長(zhǎng)江 + 地表水。 參考:《華中科技大學(xué)》2012年碩士論文


【摘要】:目的調(diào)查長(zhǎng)江武漢段地表水微生物污染現(xiàn)狀;研究地表水中菌落總數(shù)、總大腸菌群、耐熱大腸菌群、大腸埃希菌、腸球菌五種細(xì)菌學(xué)指標(biāo)之間的關(guān)系,分析其與致病菌沙門菌的相互關(guān)系,及其與溫度、pH之間的相關(guān)性,尋求最具有代表性的地表水糞便污染指示菌。方法與2011年9月-2012年5月對(duì)長(zhǎng)江武漢段地表水及同濟(jì)醫(yī)院醫(yī)院污水進(jìn)行連續(xù)采樣,采集長(zhǎng)江岸邊及江心兩固定點(diǎn)水樣和同濟(jì)醫(yī)院排污管水樣,以醫(yī)院污水作為對(duì)沙門氏菌檢測(cè)的陽(yáng)性對(duì)照組。對(duì)水中的菌落總數(shù)、總大腸菌群、耐熱大腸菌群、大腸埃希菌、腸球菌及沙門氏菌進(jìn)行定量或定性分析,并分析其相關(guān)性。結(jié)果參照《中華人民共和國(guó)地表水環(huán)境質(zhì)量標(biāo)準(zhǔn)》GB3838-2002分析,60份長(zhǎng)江水水樣中,耐熱大腸菌群的超標(biāo)率為55.0%,檢出有沙門氏菌的水樣有12份,檢出率為20.0%。結(jié)果分析顯示,,總大腸菌群、耐熱大腸菌群、大腸埃希菌密度與沙門氏菌的檢出率均呈正相關(guān),耐熱大腸菌群對(duì)腸道致病菌的提示作用最好。結(jié)論長(zhǎng)江武漢段地表水微生物污染嚴(yán)重,五種候選指示菌對(duì)致病菌污染水體均有一定的提示作用,但耐熱大腸菌群對(duì)腸道致病菌的提示作用最好,建議使用耐熱大腸菌群聯(lián)合腸球菌作為腸道致病菌污染地表水的指示菌。
[Abstract]:Objective to investigate the microbial contamination of surface water in the Wuhan section of the Yangtze River, and to study the relationship between the total colonies of the surface water, the total coliform group, the heat coliform group, the Escherichia coli and Enterococcus, and analyze the relationship between the bacteria and the pathogenic bacteria Salmonella, and the correlation between them and the temperature and pH, and seek the most representative The method of sampling the surface water of the Wuhan section of the Yangtze River and the hospital sewage of Tongji Hospital in the Yangtze River in September 2011 -2012, and collecting the water samples from the two fixed-point water samples of the Yangtze River bank and the river heart and Tongji Hospital Sewage pipe, taking the hospital sewage as the positive control group of Salmonella, and the total number of the colony in the water. The total coliform group, heat coliform group, Escherichia coli, Enterococcus and Salmonella were analyzed quantitatively and qualitatively, and their correlation was analyzed. According to the >GB3838-2002 analysis of the environmental quality standard >GB3838-2002 of the surface water of the Yangtze River, the exceeding standard rate of the heat coliform bacteria in the 60 water samples of the Yangtze river was 55%, and the water samples with Salmonella bacteria were detected. 12 samples, the detection rate of 20.0%. results showed that the total coliform group, the heat coliform bacteria group, the Escherichia coli density and the detection rate of Salmonella were positively correlated, the heat coliform group was the best indication for the intestinal pathogenic bacteria. Conclusion the surface water in the Wuhan section of the Yangtze River was polluted seriously, and the five candidate indicative bacteria contaminated the pathogenic bacteria. The body has a certain hint, but the heat coliform group has the best effect on the intestinal pathogenic bacteria. It is suggested that the heat coliform bacteria group combined with Enterococcus can be used as the indicator of the intestinal bacteria to pollute the surface water.
【學(xué)位授予單位】:華中科技大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2012
【分類號(hào)】:R123.1;X52

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本文編號(hào):2061956


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