本文選題：AR + CD36�。� 參考：《重慶醫(yī)科大學》2017年碩士論文

【摘要】：目的蘋果渣是蘋果加工成果汁、蘋果酒或葡萄酒的副產(chǎn)品。迷迭香是一種草藥,通常在食品加工中用作香料和調味劑。有證據(jù)表明,蘋果渣和迷迭香都具有豐富的生物活性分子與眾多的代謝作用。為了提供更多關于使用蘋果渣和迷迭香作為功能性食品用于改善代謝相關疾病的信息,本研究探討了蘋果渣和迷迭香提取物(a mixture of apple pomace and rosemary extract,AR)改善胰島素敏感性的作用及機制。方法33只SD雄性大鼠隨機分成正常對照組(n=6)與果糖組(n=27)。果糖組給予10%果糖水溶液。于實驗第13周,果糖組隨機分為果糖對照組,AR低、高劑量(100 mg·kg-1、500 mg·kg-1)組,每組9只,繼續(xù)果糖水溶液喂養(yǎng)。實驗周期為18周,第18周末禁食14小時后,稱體質量,大鼠脫頸處死,用ELISA法測定血漿脂質及葡萄糖代謝指標,Real-time PCR檢測相關基因表達,Western blot和免疫熒光檢測相關蛋白表達水平。結果AR(500mg/kg)治療5周抑制了長期果糖誘導所致的大鼠空腹血漿胰島素濃度的升高,降低了胰島素抵抗指數(shù)和脂肪組織胰島素抵抗指數(shù)。從總體而言,AR抑制了口服葡萄糖耐量試驗(oral glucose tolerance test,OGTT)期間果糖誘導血漿游離脂肪酸(non-esterified fatty acid,NEFA)清除率的增加,并且降低了腓腸肌中甘油三酯(triglyceride,TG)的濃度和油紅-O染色面積。此外,AR逆轉了果糖誘導肌膜上脂肪酸轉運酶/CD36(fatty acid translocase/CD36,CD36)的過表達,并且上調了肌膜上葡萄糖轉運蛋白-4(glucose transporter-4,GLUT-4)的表達,已知CD36促進脂肪酸的攝取,而GLUT-4是胰島素響應的葡萄糖載體蛋白,它們與胰島素抵抗發(fā)生密切相關。結論AR通過調控肌膜上的CD36和GLUT-4來改善果糖誘導大鼠的胰島素抵抗。
[Abstract]:Objective Apple pomace is a by-product of apple juice, cider or wine. Rosemary is an herb that is often used as a spice and seasoning in food processing. There is evidence that apple pomace and rosemary are rich in bioactive molecules and many metabolic functions. In order to provide more information on the use of apple pomace and rosemary as functional foods to improve metabolic related diseases, this study investigated the effects and mechanisms of apple pomace and rosemary extract (a mixture of apple pomace and rosemary extract AR on improving insulin sensitivity. Methods 33 Sprague-Dawley male rats were randomly divided into normal control group (n = 6) and fructose group (n = 27). Fructose group was given 10% fructose aqueous solution. At the 13th week of the experiment, fructose group was randomly divided into fructose control group with low AR and high dose (100mg kg-1500 mg kg-1) with 9 rats in each group, fed with fructose aqueous solution. The experimental period was 18 weeks. After fasting for 14 hours at the 18th week, the rats were killed by body weight. The plasma lipid and glucose metabolism index were detected by Elisa. Western blot and immunofluorescence were used to detect the expression of related genes. Results AR (500mg/kg) inhibited the increase of fasting plasma insulin concentration induced by fructose for 5 weeks, and decreased insulin resistance index and adipose tissue insulin resistance index. In general, AR inhibited the increase of free fatty acid (non-esterified fatty acididine) clearance in plasma during oral glucose tolerance test (oral glucose tolerance test), and decreased the concentration of triglyceride (TG) and the area of oil red -O staining in gastrocnemius muscle. In addition, AR reversed the overexpression of fructose induced fatty acid transporter / CD36 (fatty acid translocase / CD36 / CD36 / CD36, and up-regulated the expression of glucose transporter-4 (GLUT-4). It is known that CD36 promotes fatty acid uptake, while GLUT-4 is a glucose carrier protein responsive to insulin. They are closely associated with insulin resistance. Conclusion AR can improve insulin resistance induced by fructose by regulating CD36 and GLUT-4 on myocytes.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TS218;R151

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本文編號：2061939