發(fā)布時間：2018-06-19 21:59

  本文選題：順鉑 + 定量組織學(xué)分析��； 參考：《毒理學(xué)雜志》2015年03期

【摘要】：目的 探討順鉑(CP)染毒大鼠睪丸組織病理學(xué)和睪丸酶活力的變化。方法 選擇健康成年雄性Wistar大鼠,隨機分為CP 1.0、2.5和5.0 mg/kg組及對照組,連續(xù)腹腔注射染毒3 d。染毒結(jié)束后,制備PAS(Periodic AcidSchiff)染色病理切片,觀察睪丸組織病理變化并行定量組織學(xué)分析。分光光度法測定琥珀酸脫氫酶(SDH)、蘋果酸脫氫酶(MDH)、酸性磷酸酶(ACP)、堿性磷酸酶(AKP)、誘導(dǎo)型一氧化氮合酶(i NOS)和Na+-K+-ATPase活力。實時熒光定量PCR檢測i NOS和Na+-K+-ATPasemRNA表達水平。結(jié)果 CP染毒后大鼠睪丸組織病理學(xué)改變主要見于睪丸生精上皮精子發(fā)生周期第Ⅱ-Ⅲ期。定量組織學(xué)分析發(fā)現(xiàn),在生精上皮第Ⅱ-Ⅲ期CP 2.5和5.0 mg/kg組大鼠睪丸生精小管相對面積和直徑變小,管腔相對面積增加(P0.05)。CP各劑量組生精上皮占生精小管面積百分比和生精小管管腔直徑均變小(P0.05),CP5.0 mg/kg組睪丸間質(zhì)血管面積增加(P0.01)。與對照組比較,CP 5.0 mg/kg組精子數(shù)減少,精子活力降低(P0.05)。CP各染毒組SDH活力均低于對照組,而MDH和i NOS活力僅5.0 mg/kg組低于對照組(P0.05)。CP 5.0 mg/kg組ACP、AKP和Na+-K+-ATPase活力均明顯高于對照組(P0.05)。RT-PCR結(jié)果顯示,Na+-K+-ATPase和i NOS mRNA表達水平與酶活力改變一致。結(jié)論 順鉑可能參與改變睪丸細胞能量代謝相關(guān)酶活力而致睪丸細胞損傷。
[Abstract]:Objective to investigate the changes of testicular histopathology and testicular enzyme activity in rats exposed to cisplatin (CPD). Methods healthy adult male Wistar rats were randomly divided into 2. 5 and 5. 5 mg/kg groups of CP 1.0 and 5. 0 mg/kg and control group. The rats were injected intraperitoneally for 3 days. The pathological sections of PASA Periodic AcidSchiff staining were prepared and the pathological changes of testis were observed and the quantitative histological analysis was performed. The activities of succinate dehydrogenase (SDH), malate dehydrogenase (MDH), acid phosphatase (ACPP), alkaline phosphatase (ALP), inducible nitric oxide synthase (iNOS) and Na K ATPase were determined by spectrophotometry. The expression of iNOS and Na-K-ATPase mRNA were detected by real-time fluorescence quantitative PCR. Results the histopathological changes of testis were mainly observed in the spermatogenesis stage 鈪，

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