本文選題：菰米 + 膳食纖維��； 參考：《揚州大學》2012年碩士論文

【摘要】：目的： (1)提取菰米、玉米、蘋果、芹菜膳食纖維及菰米、玉米類黃酮,對其含量進行測定及比較,探討各類膳食纖維及類黃酮產(chǎn)生生物學效應的物質(zhì)基礎。 (2)觀察各種提取的膳食纖維、類黃酮對油酸誘導的HepG2(?)(?)酒精性脂肪肝細胞漿內(nèi)的甘油三酯(TG)、丙二醛(MDA)、超氧化物歧化酶(SOD)的含量的影響,探討不同種類的膳食纖維、類黃酮對非酒精性肝脂肪變性細胞作用的效果差異。 (3)觀察非酒精性肝脂肪變性細胞內(nèi)過氧化物酶體增殖體激活受體-α(PPAR-α)、肉堿脂酰轉(zhuǎn)移酶-1A(CPT-1A)在mRNA水平上的影響,探討菰米、玉米、芹菜、蘋果膳食纖維及菰米、玉米類黃酮對非酒精性肝脂肪變性細胞的作用機制。 方法： (1)用酶-化學法提取菰米、玉米中膳食纖維,用化學法提取蘋果、芹菜的膳食纖維,按國標法對其含量進行測定；用浸提回流法提取菰米、玉米的類黃酮,用比色法對其含量進行測定。 (2)用油酸誘導HepG2細胞建立體外非酒精性肝脂肪變性模型,通過MTT、油紅O染色及檢測細胞漿內(nèi)TG的含量,來確定油酸的濃度。 (3)油酸誘導HepG2細胞脂肪變性,加入菰米、玉米、芹菜、蘋果膳食纖維及菰米、玉米類黃酮,觀察對細胞漿內(nèi)TG、MDA、SOD含量的變化；同時測定細胞內(nèi)PPAR-a及CPT-1AmRNA的表達。 結果： (1)菰米膳食纖維總膳食纖維(TDF)的含量7.242g/100g,玉米TDF的含量9.13g/100g,均高于大米；蘋果TDF占預處理蘋果粉的含量為13.52%,芹菜TDF占預處理芹菜粉的含量為38.6%；菰米類黃酮的含量3.674g/100g,顯著高于玉米類黃酮的含量。 (2)0.5mM的油酸對細胞活性無顯著影響,且細胞內(nèi)TG顯著升高,細胞內(nèi)脂滴蓄積明顯,是誘導脂肪變性的最佳濃度。 (3)100μg/ml菰米、玉米膳食纖維能降低HepG2非酒精性肝脂肪變性細胞漿中TG、MDA含量,升高SOD含量,對非酒精性肝脂肪變性具有一定的改善作用,芹菜膳食纖維的作用次之,而蘋果膳食纖維的作用不明顯；100μg/ml菰米、玉米類黃酮也降低模型細胞漿內(nèi)的TG含量。 (4)與非酒精性肝脂肪變性細胞模型組相比,菰米、玉米、芹菜膳食纖維及菰米、玉米類黃酮處理的細胞內(nèi)PPAR-a及CPT-1A mRNA的表達顯著增高(P0.05),而蘋果膳食纖維PPAR-a及CPT-1A mRNA的表達稍有增高,但沒有統(tǒng)計學意義(P0.05)。 結論： (1)0.5mM的油酸與HepG2細胞孵育24小時,能誘導脂肪變性,形成非酒精性肝脂肪變性細胞模型。 (2)菰米、玉米膳食纖維及類黃酮對非酒精性肝脂肪變性細胞具有一定的改善作用,作用的機制可能是激活PPAR-a,促進CPT-1A的表達,增加脂肪酸分解,減少體脂含量,延緩脂肪肝形成。 (3)芹菜膳食纖維也能改善非酒精性肝脂肪變性,而蘋果膳食纖維的作用則較小。可能的原因是,膳食纖維的不同來源,所含的可溶性膳食纖維和不可溶性的膳食纖維的含量不同,膳食纖維相結合的植物化學物不同,而導致的作用有所差異。
[Abstract]:Objective: to extract Zizania latifolia, corn, apple, celery dietary fiber, Zizania rice and corn flavonoids, and compare them with each other. Study on the material basis of biological effects of dietary fiber and flavonoids. The effects of triglyceride, malondialdehyde (MDA) and superoxide dismutase (SOD) on the contents of triglyceride (TGG), malondialdehyde (MDA) and superoxide dismutase (SOD) in the cytoplasm of alcoholic fatty liver were studied. The effects of flavonoids on non-alcoholic hepatic steatosis cells were studied. The effects of peroxisome proliferator activator receptor-偽 -PPAR- 偽, carnitine acid-transferase -1AnCPT-1A) on the mRNA level in non-alcoholic hepatic steatosis cells were observed. To investigate the mechanism of effects of Zizania latifolia, maize, celery, apple dietary fiber, Zizania rice and zeatin flavonoids on non-alcoholic hepatic fatty degeneration cells. Methods: 1) extract the dietary fiber from Zizania latifolia, corn, apple and celery by enzymatic chemical method, and extract the flavonoids from Zizania rice and corn by extraction and reflux method, and determine the content of dietary fiber of apple and celery by chemical method, and extract the flavonoids of Zizania rice and corn by extraction and reflux method. HepG2 cells were induced by oleic acid to establish a model of non-alcoholic fatty degeneration of liver in vitro. The content of TG in the cytoplasm was detected by MTT, oil red O staining. To determine the concentration of oleic acid. 3) oleic acid induced fatty degeneration of HepG2 cells, adding Zizania latifolia, corn, celery, apple dietary fiber and Zizania rice, corn flavonoids to observe the changes of SOD content in cytoplasm. At the same time, the expression of PPAR-a and CPT 1 A mRNA were measured. Results: (1) the content of total dietary fiber (TDF) of Zizania latifolia was 7.242 g / 100 g, the content of TDF of maize was 9.13 g / 100 g, the content of TDF of apple was 13.52%, the content of TDF of celery was 38.6%, the content of TDF of apple was 13.52%, the content of TDF of celery was 38.6%. The content of flavonoids in Zizania latifolia was 3.674g / 100g, which was significantly higher than that of corn flavonoids. It is the best concentration to induce steatosis. The corn dietary fiber can decrease the content of TG-MDA and increase the content of SOD in the cytoplasm of HepG2 non-alcoholic hepatic steatosis, and it can improve the non-alcoholic liver steatosis. The effect of celery dietary fiber was the second, while the effect of apple dietary fiber was not obvious. Corn flavonoids also decreased the TG content in the cytoplasm of the model cells. The expression of PPAR-a and CPT-1A mRNA in maize, celery dietary fiber and Zizania rice, maize flavonoid treated cells was significantly increased, while the expression of PPAR-a and CPT-1A mRNA in apple dietary fiber was slightly increased, but there was no significant difference in the expression of PPAR-a and CPT-1A mRNA, but there was no statistically significant difference in the expression of PPAR-a and CPT-1A mRNA. Conclusion: 0. 5 mm oleic acid can induce fatty degeneration in HepG2 cells for 24 hours and form a non alcoholic hepatosteatosis cell model. Corn dietary fiber and flavonoids can improve non-alcoholic liver steatosis cells by activating PPAR-a, promoting CPT-1A expression, increasing fatty acid decomposition, and reducing body fat content, the mechanism of which may be the activation of PPAR-a, the increase of CPT-1A expression, the increase of fatty acid decomposition, and the decrease of body fat content. The celery dietary fiber can also improve the non-alcoholic hepatic steatosis, but the apple dietary fiber has little effect. The possible reason is that different sources of dietary fiber, the content of soluble dietary fiber and insoluble dietary fiber, and different phytochemicals combined with dietary fiber lead to different effects.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R151.3

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