本文選題：細(xì)胞周期 + 細(xì)胞增殖��； 參考：《浙江大學(xué)》2013年碩士論文

【摘要】：背景：社會(huì)的現(xiàn)代化以及普遍化使得電力設(shè)備成為人們生活不可或缺的部分,卻由此帶來了人們?nèi)粘Ｉ詈凸ぷ鳝h(huán)境中極低頻電磁場水平的大幅增加,電力設(shè)施等產(chǎn)生的多為工頻磁場,引發(fā)人們對工頻電磁場生物效應(yīng)的關(guān)注和探索,正確客觀的評價(jià)工頻磁場的生物學(xué)效應(yīng)成為公共衛(wèi)生領(lǐng)域亟待解決的科學(xué)問題。流行病學(xué)研究、動(dòng)物學(xué)實(shí)驗(yàn)及細(xì)胞學(xué)實(shí)驗(yàn)表明極低頻磁場可能存在健康相關(guān)的生物學(xué)影響,相比于電磁場暴露的即時(shí)效應(yīng)研究,對于暴露后的時(shí)段里電磁場是否會(huì)對機(jī)體有后續(xù)的生物學(xué)影響卻少有涉及。細(xì)胞增殖是細(xì)胞生命過程中的重要進(jìn)程,也是生物學(xué)研究中重要的觀測指標(biāo),細(xì)胞周期的分布可以反映細(xì)胞對外界刺激的敏感性,本研究擬觀察工頻磁場暴露的后續(xù)時(shí)間內(nèi)細(xì)胞增殖和細(xì)胞周期的變化情況,以期初步評價(jià)不同暴露條件下工頻磁場的后續(xù)生物學(xué)效應(yīng),為該領(lǐng)域的研究提供參考。 目的：選用細(xì)胞增殖和細(xì)胞周期為效應(yīng)指標(biāo),初步評價(jià)工頻磁場暴露后的后續(xù)生物學(xué)效應(yīng)。 方法：MEF細(xì)胞接種后預(yù)培養(yǎng)約12h,暴露于50Hz的工頻磁場中,暴露條件分別為：暴露強(qiáng)度為0.5mT、2mT,暴露模式為powerline及sine,暴露時(shí)間為8h和24h。采用CCK-8試劑盒法和流式細(xì)胞術(shù)分別檢測暴露結(jié)束后的0h、12h、24h、36h、48h時(shí)的細(xì)胞增殖和細(xì)胞周期分布情況。各暴露條件均設(shè)有一一對照,每個(gè)實(shí)驗(yàn)重復(fù)至少3次。 結(jié)果：(1)CCK-8試劑盒檢測細(xì)胞增殖的實(shí)驗(yàn)結(jié)果：用SPSS16.0軟件對實(shí)驗(yàn)檢測結(jié)果進(jìn)行協(xié)方差分析,在本實(shí)驗(yàn)條件下,工頻磁場暴露后0-48h未對細(xì)胞增殖產(chǎn)生影響；(2)采用流式細(xì)胞術(shù)檢測MEF細(xì)胞周期的實(shí)驗(yàn)結(jié)果：50Hz、0.5mT工頻磁場powerline模式下輻照24h,發(fā)現(xiàn)在輻照結(jié)束后的第24h,暴露組細(xì)胞出現(xiàn)了細(xì)胞周期的G2/M期阻滯；而在sine模式下輻照8h和24h,卻發(fā)現(xiàn)在暴露結(jié)束后的第24h和第48h,暴露組與對照組相比分布在G0/G1期的細(xì)胞比例減少；50Hz、2mT工頻磁場powerline模式及sine模式暴露24h,發(fā)現(xiàn)在輻照結(jié)束后的第36h,暴露組細(xì)胞出現(xiàn)了細(xì)胞周期的S期阻滯。 結(jié)論：本實(shí)驗(yàn)應(yīng)用細(xì)胞周期和細(xì)胞增殖兩個(gè)效應(yīng)指標(biāo)評價(jià)工頻磁場的后續(xù)生物學(xué)作用,在本實(shí)驗(yàn)條件下未觀察到工頻磁場作用引起明顯后續(xù)生物學(xué)效應(yīng)。
[Abstract]:Background: with the modernization and generalization of society, electric power equipment has become an indispensable part of people's lives, but it has brought about a significant increase in the level of very low frequency electromagnetic fields in people's daily life and working environment. Most of the electric power facilities are power frequency magnetic field, which has aroused people's attention and exploration to the biological effect of power frequency electromagnetic field. The correct and objective evaluation of the biological effect of power frequency magnetic field has become a scientific problem to be solved urgently in the field of public health. Epidemiological, zoological and cytological studies have shown that very low frequency magnetic fields may have health-related biological effects, compared with the immediate effects of electromagnetic field exposure. There is little concern about whether the electromagnetic field will have subsequent biological effects on the body during the period after exposure. Cell proliferation is an important process in the course of cell life and an important observation index in biological research. The distribution of cell cycle can reflect the sensitivity of cells to external stimuli. The aim of this study was to observe the changes of cell proliferation and cell cycle during the follow-up time of power frequency magnetic field exposure, in order to evaluate the following biological effects of power frequency magnetic field under different exposure conditions, and to provide a reference for the study in this field. Aim: to evaluate the subsequent biological effects of power frequency magnetic field exposure using cell proliferation and cell cycle. Methods the cells were precultured for 12 hours after inoculation and exposed to 50 Hz power frequency magnetic field. The exposure conditions were as follows: the exposure intensity was 0.5mTX 2mT, and the exposure mode was powerline and Sine. the exposure time was 8h and 24h, respectively. CCK-8 kit and flow cytometry were used to detect cell proliferation and cell cycle distribution at 0 h ~ 12 h ~ 24 h ~ 36 h ~ 48 h after exposure. Each exposure condition was controlled one by one, and each experiment was repeated at least three times. Results the cell proliferation was detected by the CCK-8 Kit. SPSS 16.0 software was used to analyze the covariance of the results. Under the experimental conditions, 0-48 h after exposure to the power frequency magnetic field, the cell proliferation was not affected. (2) flow cytometry was used to detect the cell cycle of MEF cells. The results showed that the G _ 2 / M phase arrest of cell cycle appeared in the exposed group at 24 h after irradiation with 0.5 Mt power frequency magnetic field powerline mode. However, the proportion of cells distributed in G _ 0 / G _ 1 phase decreased in the exposed group at 24 h and 48 h after irradiation in sine mode for 8 h and 24 h, respectively. 50 Hz ~ 2mT power frequency magnetic field powerline mode and sine mode were exposed for 24 h. It was found that S phase arrest of cell cycle appeared in the exposed group at 36h after irradiation. Conclusion: the following biological effects of power frequency magnetic field were evaluated by cell cycle and cell proliferation in this experiment.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R131

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本文編號：2015853